| Project/Area Number |
15H06318
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Kubota Ryou 京都大学, 工学研究科, 助教 (00753146)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 金属錯体 / 化学遺伝学 / GPCR / リガンド作動性イオンチャネル / 遺伝子工学 / 膜タンパク質受容体 / 部位特異的変異導入 / Gタンパク質共役受容体 / グルタミン酸受容体 / アドレナリン受容体 / 超分子化学 / 蛋白質 / シグナル伝達 / 分子認識 / 細胞・組織 / 配位結合 / ケミカルバイオロジー |
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| Outline of Final Research Achievements |
Membrane-bound receptors in living cells mediate signal transductions by receiving extracellular signals. Despite their biological importance, it is still quite difficult to unveil their functions due to their similar and complicated three-dimensional structures and activation mechanisms. Here we have developed novel chemogenetic approaches, called On-cell Coordination Chemistry (OcCC) in order to artificially control the functions of membrane-bound receptors. We successfully demonstrated that OcCC enabled to control the activities of two types of neurotransmitter receptors, ionchannel type and GPCR type glutamate receptors, in live neurons. Also, we achieved the artificial activation of class A GPCRs by coordination anchor method.
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