| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
I analyzed the functions of five homologs (SlPlsC1-SlPlsC5) of 1-acyl-sn-glycerol-3-phosphate acyltransferase (PlsC) from the psychrotrophic bacterium Shewanella livingstonensis Ac10. 1) I established the methods of purification and enzymatic assay for SlPlsC1 and revealed its enzymatic characteristics in vitro. 2) It was indicated that SlPlsC3 is involved in the biosynthesis of a yellow pigment of which the chemical structure is as yet unidentified, suggesting a novel function of PlsC-family enzymes. In addition, 3) aiming at the crystal structure analysis of PlsC, I purified a thermostable PlsC from the thermophilic bacterium Thermus thermophilus and revealed its enzymatic characteristics.
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