Elucidation of the effect of mitochondrial complex I on epithelial mesenchymal transition in breast cancer

• Project/Area Number: 15H06338
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: Matsumoto Yoshiaki 京都大学, 医学研究科, 特定助教 (80760769)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: がん幹細胞性 / 足場非依存性 / 乳癌 / 癌 / EMT / 代謝

Outline of Final Research Achievements

We hypothesized that metabolic function of breast cancer is modified when its mitochondrial complex gene changes, resulting in acquisition of cancer stemness (especially anchorage independent growth ability). We examined anchorage independent growth ability of breast cancer cell line with modified metabolic function. Changes in the metabolism of breast cancer cell line accompanying epithelial mesenchymal transition are under analysis.
We newly identified a gene which is thought to be involved in anchorage independent growth and anoikis resistance. The gene exists downstream of a certain gene related to cell migration ability. We revealed that this gene is involved in cancer stemness via CD44 variant.

Research Products

• [Journal Article] Visualization of tumor-related blood vessels in human breast by photoacoustic imaging system with a hemispherical detector array (2017)
  • Author(s): Masakazu Toi, Yasufumi Asao, Yoshiaki Matsumoto et al
  • Journal Title: Scientific Reports Volume: 7 Issue: 1
  • DOI: 10.1038/srep41970
  • NAID: 120005980817
  • Peer Reviewed / Open Access
• [Journal Article] 光超音波技術の乳癌診断への応用 (2016)
  • Author(s): 松本純明, 浅尾恭史, 金尾昌太郎, 片岡正子, 椎名毅, 戸井雅和
  • Journal Title: 乳癌の臨床 Volume: 31 Pages: 7-17
• [Presentation] Abstract P4-01-10: Development of photoacoustic vascular imaging system for breast cancer (2016)
  • Author(s): Masakazu Toi, Yoshiaki Matsumoto et al
  • Organizer: San Antonio Breast Cancer Symposium 2016
  • Place of Presentation: San Antonio
  • Year and Date: 2016-12-06
  • Int'l Joint Research
• [Presentation] プレジデンシャルシンポジウム2 若い力が拓く明日の乳がん医療 (2016)
  • Author(s): 松本純明
  • Organizer: 第24回日本乳癌学会学術総会
  • Place of Presentation: 東京ビッグサイト
  • Year and Date: 2016-06-16
  • Invited
• [Presentation] 乳癌治療標的を目指したがん代謝研究 (2015)
  • Author(s): 松本 純明
  • Organizer: 第13回日本乳癌学会 近畿地方会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2015-11-28
  • Invited