| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Hematopoietic stem cells (HSCs) maintain the turnover of mature blood cells in bone marrow (BM). The cell-cycle status of HSCs are known to fluctuate according to physiological circumstances. However, precise mechanisms regulating HSCs status remain elusive.We performed dynamic live imaging of BM to analyze homeostatic or activated HSCs using multiphoton microscopy. The mean velocity and displacement of HSCs transplanted into 5-fluorouracil (5-FU) treated mice were significantly decreased compared to those transplanted into control mice. Moreover, most of transplanted HSCs in 5-FU treated recipients were adjacent to endothelial cells. Gene expression analyses revealed that expression levels of cell-cycle related genes in transplanted cells were different between these 2 recipient groups. These results suggested that microenvironments after BM injury supply proliferative signals to HSCs and influence cell-cycle status of them.
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