• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Streptococcus pyogenes endopeptidase O contributes to evasion from complement-mediated bacteriolysis via human complement factor C1q

Research Project

Project/Area Number 15H06380
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionOsaka University

Principal Investigator

Ogawa Mariko  大阪大学, 歯学研究科, 特任研究員 (20754732)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords化膿レンサ球菌 / 補体 / エンドペプチダーゼ / 古典経路 / C1q / 古典系路
Outline of Final Research Achievements

In this study, we identified an endopeptidase of Streptococcus pyogenes, PepO, as an interacting molecule with complement C1q, and investigated its effects on complement immunity and pathogenesis.
ELISA results revealed that PepO bound to C1q in a concentration-dependent manner under physiological conditions. Then, the effects of PepO on bacterial evasion from complement immunity was analyzed in various pH condition. Under low pH conditions, PepO inhibited the binding of IgG to C1q. In addition, pepO deletion rendered S. pyogenes more susceptible to the bacteriocidal activity of human serum. The wild-type strain (WT)-infected tissues exhibited greater severity and lower complement activity as compared to those infected by ΔpepO in a mouse skin infection model.
Our results suggest that interaction of S. pyogenes PepO with C1q interferes with the complement pathway, which enables S. pyogenes to evade complement-mediated bacteriolysis under acidic conditions.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (8 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] treptococcus pyogenes Endopeptidase O Contributes to Evasion from Complement-mediated Bacteriolysis via Binding to Human Complement Factor C1q2017

    • Author(s)
      Honda-Ogawa M., Sumitomo T., Mori Y., Hamd DT, Ogawa T., Yamaguchi M., Nakata M., Kawabata S.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 292 Issue: 10 Pages: 4244-4254

    • DOI

      10.1074/jbc.m116.749275

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 化膿レンサ球菌のendopeptidase Oと補体C1qの相互作用が病原性に及ぼす影響2017

    • Author(s)
      本多 真理子,住友 倫子,山口 雅也,中田 匡宣,川端 重忠
    • Organizer
      第90回 日本細菌学会総会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] C1qとの相互作用を介する化膿レンサ球菌の補体免疫回避機構2016

    • Author(s)
      小川 真理子,住友 倫子,毛利 泰士,山口 雅也,中田 匡宣,川端 重忠
    • Organizer
      第58回 歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Streptococcus pyogenesが産生するendopeptidase OとC1qの結合による補体免疫回避機構2016

    • Author(s)
      本多 真理子,住友 倫子,毛利 泰士,Dalia Hamd, 山口 雅也,中田 匡宣, 川端 重忠
    • Organizer
      第48回レンサ球菌研究会
    • Place of Presentation
      長崎大学(長崎県長崎市)
    • Year and Date
      2016-07-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Involvement of Group A streptococcal endopeptidase O in evasion from complement-mediated bacteriolysis via binding to complement C1q.2016

    • Author(s)
      M. Honda, T. Sumitomo, Y. Mori, M. Yamaguchi, M. Nakata, S. Kawabata.
    • Organizer
      The 13th Korea - Japan International Symposium on Microbiology (X-KJISM)
    • Place of Presentation
      慶州(韓国)
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of Group A streptococcal endopeptidase O in evasion from complement-mediated bacteriolysis via binding to complement C1q2016

    • Author(s)
      M. Honda, T. Sumitomo, Y. Mori, M. Yamaguchi, M. Nakata, S. Kawabata
    • Organizer
      第13回 韓日微生物学シンポジウム
    • Place of Presentation
      慶州市,慶北,韓国,K-Hotel Gyeongju
    • Year and Date
      2016-05-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A群レンサ球菌のendopeitidase Oによる補体C1qを介した補体免疫回避機構2016

    • Author(s)
      本多 真理子,住友 倫子,毛利 泰士,山口 雅也,中田 匡宣,川端 重忠
    • Organizer
      第89回 日本細菌学会総会
    • Place of Presentation
      大阪市,大阪府,日本,大阪国際交流センター
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 補体C1qを介した化膿レンサ球菌の補体免疫回避機構の解明2015

    • Author(s)
      小川 真理子
    • Organizer
      第3回 四大学・口腔微生物研究会
    • Place of Presentation
      新潟市,新潟県,日本,ホテルメッツ新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-08-26   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi