Virus-mediated suppression of plant innate immunity

• Project/Area Number: 15H06420
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant protection science
• Research Institution: Okayama University
• Principal Investigator: Hyodo Kiwamu 岡山大学, 資源植物科学研究所, 助教 (80757881)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物ウイルス / 植物免疫 / 活性酸素種 / カルシウム依存性タンパク質キナーゼ / ウイルス誘導性免疫不全 / ROSバースト / RBOHタンパク質 / 宿主因子 / RCNMV / ３量体Gタンパク質 / ゲノムRNA複製

Outline of Final Research Achievements

Plants have a multitier surveillance system that recognizes invading microbial pathogens using plasma membrane-localized and intracellular immune receptors. An RBOH (respiratory burst oxidase homolog) protein plays a key role in plant innate immunity. In this study, we found that a plant virus hijacks a host ROS generating enzyme RBOH for its multiplication. We also found that a plant virus infection can interfere with host immune responses.

Research Products

• [Journal Article] Harnessing host ROS-generating machinery for the robust genome replication of a plant RNA virus. (2017)
  • Author(s): Hyodo K, Hashimoto K, Kuchitsu K, Suzuki N, Okuno T
  • Journal Title: Proceedings of the National Academy of Science, U.S.A. Volume: 114 Issue: 7 Pages: 1282-1290
  • DOI: 10.1073/pnas.1610212114
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Pathogenesis mediated by proviral host factors involved in translation and replication of plant positive-strand RNA viruses. (2016)
  • Author(s): Hyodo K and Okuno T.
  • Journal Title: Current Opinion in Virology Volume: 17 Pages: 11-18
  • DOI: 10.1016/j.coviro.2015.11.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 毒薬変じて薬となる？～植物を助けるウイルス～ (2015)
  • Author(s): 兵頭究
  • Journal Title: 生物工学 Volume: 93 Pages: 4-4
• [Presentation] イネの傷害誘導性ペプチドOsPep3は植食性昆虫エリシター応答を増強する (2017)
  • Author(s): 新屋友規・北條優子・兵頭究・晝間敬・西條雄介・Galis Ivan
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学群元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] A role of reactive oxygen species during the replication of a plant RNA virus (2017)
  • Author(s): Hyodo K.
  • Organizer: International Mini-symposium Series II on Fungal and Plant Viruses
  • Place of Presentation: IPSR, Okayama University （Kurashiki, Okayama）
  • Year and Date: 2017-03-08
  • Int'l Joint Research / Invited
• [Presentation] ウイルス感染による植物免疫への干渉作用 (2016)
  • Author(s): 兵頭究
  • Organizer: 菌類／植物ウイルス研究ミニシンポジウム
  • Place of Presentation: 岡山大学資源植物科学研究所（岡山県倉敷市）
  • Year and Date: 2016-09-21
  • Invited
• [Presentation] A role of host second messengers in dianthovirus replication (2016)
  • Author(s): Hyodo K.
  • Organizer: International Mini-Workshop on Plant Virus Research
  • Place of Presentation: 岡山大学資源植物科学研究所 （倉敷市）
  • Year and Date: 2016-03-25
  • Int'l Joint Research / Invited
• [Presentation] 活性酸素種は植物RNAウイルス増殖を正に制御する (2016)
  • Author(s): 兵頭究 鈴木信弘 奥野哲郎
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター （岡山市）
  • Year and Date: 2016-03-21
• [Presentation] 植物ーウイルス間相互作用における活性酸素種の役割 (2016)
  • Author(s): 兵頭究
  • Organizer: 第８回植物ストレス科学研究シンポジウム
  • Place of Presentation: 倉敷芸文館 アイシアター （倉敷市）
  • Year and Date: 2016-03-07
  • Invited
• [Remarks] 岡山大学資源植物科学研究所 植物・微生物相互作用グループ
  • URL: http://www.rib.okayama-u.ac.jp/pmi/publication.html