| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Rice requires high silicon (Si) for its high and sustainable yield. Two Si transporters Lsi1 and Lsi2 are involved in this high accumulation of Si in rice. Our previous studies showed that the mRNA expression levels of these transporter genes were down-regulated by Si. In this project I investigated the mechanism underlying regulation of Lsi1 and Lsi2 expression. There was a negative correlation between the expression level of Lsi1 and Lsi2 and shoot Si accumulation. Together with the results of split-root experiments, the Si-induced down-regulation of Si transporter genes is controlled by shoot Si accumulation, not root Si. Analysis with transgenic rice carrying different lengths of Lsi1 promoter regions fused with GFP as a reporter gene revealed that the region responsible for Si response of Lsi1 expression is present between -327 to -292 in the promoter.
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