| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To elucidate the role of TFG in insulin secretion, we generated pancreatic β-cell specific TFG knockout mice (βTFG KO) and analyzed the phenotype. βTFG KO displayed marked hyperglycemia with impaired insulin secretion on glucose tolerance test. Impairment of both β-cell mass and function was suggested by smaller insulin-positive area in the immunohistochemical analysis of the pancreas and reduced insulin secretion on glucose stimulation in isolated islets. Electron microscopic observation and microarray gene expression analysis revealed smaller insulin crystal diameters, mild ER stress and downregulation of Nrf2 and its downstream genes as possible underlying mechanisms for the impaired β-cell mass and function in βTFG KO.
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