Research Project
Grant-in-Aid for Research Activity Start-up
The skin of patients with atopic dermatitis (AD) presents as a disbalance of the microbiome with a strong colonization by Staphylococcus aureus. However, the effect of colonized S. aureus on the skin immune system has not been fully elucidated. We explored whether S. aureus TF3378 isolated from AD skin was able to skew T cell responses via Langerhans cells (LC) as compared to a standard strain of S. aureus NCTC8325. Monocyte-derived LC (MoLC) from healthy controls and patients with AD were stimulated with S. aureus NCTC8325 or TF3378. S. aureus-stimulated MoLC were co-cultured with autologous CD4pos T cells and then T cell responses were analyzed. MoLC stimulated by S. aureus TF3378 induced significantly high and rapid proliferation of T cells with less Th1 cytokine (IFN-γ) production. The mRNA from T cells after co-culture showed imbalanced Th1/Th2 expression. These data illustrate S. aureus TF3378 on AD skin can skew T cell responses via LC toward imbalanced Th1 / Th2 skin immunity.
All 2017 2016 2015
All Presentation (3 results)
