Research on brown adipocyte for controlling of obesity

• Project/Area Number: 15H06433
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Oue Kana 広島大学, 病院(歯), 歯科診療医 (60760329)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 褐色脂肪細胞 / エネルギー代謝 / UCP1 / 肥満

Outline of Final Research Achievements

Phospholipase C-related catalytically inactive protein (PRIP) was first identified as an inositol 1,4,5-trisphosphate-binding protein. In this study, I examined the mechanism of PRIP-mediated energy consumption by analysing PRIP knockout (Prip-KO) mice. Prip-KO mice showed higher ability to consume fatty acids as an energy source and higher activity in non-shivering thermogenesis in brown adipocytes than wild-type mice. Energy consumption in Prip-KO beige adipocytes, which are differentiated from subcutaneous white adipocytes in response to cold exposure, was also upregulated. Collectively, these results indicate that PRIP is a new modulator for regulating the systemic energy expenditure. Hence, this increased understanding of non-shivering thermogenic mechanisms via PRIP offers important insights for the treatment and control of obesity.

Research Products

• [Journal Article] Phospholipase C-related catalytically inactive protein can regulate obesity, a state of peripheral inflammation. (2017)
  • Author(s): Yamawaki Y, Oue K, Shirawachi S, Asano S, Harada K, Kanematsu T.
  • Journal Title: Japanese Dental Science Review Volume: 53（1） Issue: 1 Pages: 18-24
  • DOI: 10.1016/j.jdsr.2016.06.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phospholipase C-related catalytically inactive protein-knockout mice display increased induction of uncoupling protein 1 in adipose tissues following chronic cold exposure. (2017)
  • Author(s): Kana Oue, Yosuke Yamawaki, Satoshi Asano, Mizokami Akiko, Masato Hirata, Masahiro Irifune, Takashi Kanematsu.
  • Journal Title: Journal of Oral Biosciences Volume: 印刷中 Issue: 2 Pages: 108-112
  • DOI: 10.1016/j.job.2017.04.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phospholipase C-related catalytically inactive protein is a new modulator of thermogenesis promoted by β-adrenergic receptors in brown adipocytes (2015)
  • Author(s): Kana Oue, Jun Zhang, Kae Harada-Hada, Satoshi Asano, Yosuke Yamawaki, Masaki Hayashiuchi, Hisako Furusho, Takashi Takata, Masahiro Irifune, Masato Hirata, Takashi Kanematsu
  • Journal Title: Journal of Biological Chemistry Volume: 291 Issue: 8 Pages: 4185-96
  • DOI: 10.1074/jbc.m115.705723
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] New molecular basis in the regulation of lipolysis via dephosphorylation (2015)
  • Author(s): 大植香菜， 原田佳枝， 兼松隆
  • Journal Title: Folia Pharmacologica Japonica Volume: 146 Issue: 2 Pages: 93-97
  • DOI: 10.1254/fpj.146.93
  • NAID: 130005092136
  • ISSN: 0015-5691, 1347-8397
  • Peer Reviewed / Open Access
• [Presentation] 寒冷刺激で見られるPRIP遺伝子欠損マウスのUCP1発現の亢進 (2017)
  • Author(s): 大植 香菜、入舩 正浩、兼松 隆
  • Organizer: 第131回日本薬理学会近畿部会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2017-06-30