• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of upstream signaling pathway responsible for primordial germ cell specification

Research Project

Project/Area Number 15H06447
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionThe University of Tokushima

Principal Investigator

OKAMURA Eiichi  徳島大学, 大学院医歯薬学研究部(医学系), 助教 (30755913)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsEsrrb / Bmp4 / 始原生殖細胞 / 栄養膜幹細胞 / 胚体外外胚葉 / CRISPR/Cas9
Outline of Final Research Achievements

It is well known that Bmp4 signaling from extraembryonic ectoderm (ExE) is required for primordial germ cell (PGC) specification in the embryo proper. However, the mechanisms to regulate Bmp4 gene expression in ExE has not been well characterized. In this study, I demonstrated that an orphan nuclear receptor Esrrb (estrogen related receptor beta) directly regulates Bmp4 gene expression in the ExE. Deletion of Esrrb-bound Bmp4 enhancer region resulted in decreasing of Bmp4 secretion from ExE and reduction of PGC number in mid-gestation embryos. Thus, it was clearly demonstrated that Esrrb is one of the critical upstream factors regulating PGC specification through regulation of Bmp4 signaling.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report

Research Products

(2 results)

All 2015 Other

All Int'l Joint Research (1 results) Presentation (1 results)

  • [Int'l Joint Research] The Hospital for Sick Children(Canada)

    • Related Report
      2016 Annual Research Report
  • [Presentation] 転写因子EsrrbとCdx2による栄養膜幹(TS)細胞特異的遺伝子発現制御2015

    • Author(s)
      岡村永一, Lingyu Li, Jodi Garner, Oliver Tam, Janet Rossant
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際展示場(兵庫県・神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-08-26   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi