| Project/Area Number |
15H06473
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | The University of Tokyo (2016)
Kyushu University (2015) |
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Principal Investigator |
Matsui Teppei 東京大学, 大学院医学系研究科(医学部), 助教 (10725948)
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| Research Collaborator |
MURAKAMI Tomonari
HASHIMOTO Takayuki
UEMURA Masato
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 視覚 / 二光子 / カルシウムイメージング / 二光子イメージング / 神経科学 |
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| Outline of Final Research Achievements |
There were two technical challenges that need to be overcome. First goal was to establish a method to express GCaMP in marmoset cortical neurons at a level sufficient for in vivo imaging. We accomplished this goal by finding an appropriate promoter for driving GCaMP expression in marmoset neurons. Second goal was to have columner level functional mapping over marmoset visual cortex. We confirmed that traditional intrinsic signal optical imaging can meet this goal. We further established a wide-field calcium imaging technique that far exceed the intrinsic signal imaging in both temporal resolution and signal to noise ratio. Combination of these two techniques will enable functional mapping of top down signal in the marmoset visual cortex.
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