Search for diagnosis and therapy of oral cancer targeting TRP channles

• Project/Area Number: 15H06497
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Saga University
• Principal Investigator: Reona Aijima 佐賀大学, 医学部, 助教 (30756143)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 口腔癌 / 温度感受性 / TRPチャネル

Outline of Final Research Achievements

Intracellular calcium plays an important role in cancer progression and metastasis. In this study, we investigated the role of transient receptor potential (TRP) channels, Ca2+-permeable nonselective cation channels, in oral squamous cell carcinoma (OSCC).HSC4, well-differentiated OSCC cell line, highly expressed functional TRPV4 channel. Knockdown of TRPV4 showed the change of localization of the adherens junction molecules. Furthermore, TRPV4 silencing induced enhancement of migration in HSC4. Additionally, TRPV4 activation led cell death presumably via JNK/p38 pathway.
These results suggest that TRPV4 wound be a novel therapeutic target for oral cancer therapy.

Research Products

• [Journal Article] Inactivation effect of DBD on oral cancer cells inside culture medium film (2016)
  • Author(s): Ono R, Ohtsubo T, Hayashi N, Aijima R, Yamashita Y, Goto M
  • Journal Title: J. Photopolym. Sci. Tec. Volume: 29 Pages: 443-445
  • Peer Reviewed
• [Journal Article] The thermosensitive TRPV3 channel contributes to rapid wound healing in oral epithelia (2015)
  • Author(s): Aijima R, Wang B, Takao T, Mihara H, Kashio M, Ohsaki Y, Zhang JQ, Mizuno A, Suzuki M, Yamashita Y, Masuko S, Goto M, Tominaga M, Kido MA
  • Journal Title: The FASEB Journal Volume: 29 Issue: 1 Pages: 182-192
  • DOI: 10.1096/fj.14-251314
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transplantation of mesenchymal stem cells ameliorates secondary osteoporosis through interleukin-17-impaired functions of recipient bone marrow mesenchymal stem cells in MRL/lpr mice. (2015)
  • Author(s): Ma L, Aijima R, Hoshino Y, Yamaza H, Tomoda E, Tanaka Y, Sonoda S, Song G, Zhao W, Nonaka K, Shi S, Yamaza T
  • Journal Title: Stem Cell Res. Ther. Volume: 6(1) Issue: 1 Pages: 182-182
  • DOI: 10.1186/s13287-015-0091-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In vivo hepatogenic capacity and therapeutic potential of stem cells from human exfoliated deciduous teeth in liver fibrosis in mice. (2015)
  • Author(s): Yamaza T, Alatas FS, Yuniartha R, Yamaza H, Fujiyoshi JK, Yanagi Y, Yoshimaru K, Hayashida M, Matsuura T, Aijima R, Ihara K, Ohga S, Shi S, Nonaka K, Taguchi T.
  • Journal Title: Stem Cell Res Ther Volume: 6(1) Issue: 1 Pages: 171-171
  • DOI: 10.1186/s13287-015-0154-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Milk basic protein supplementation enhances fracture healing in mice (2015)
  • Author(s): Yoneme H, Hatakeyama J, Danjo A, Oida H, Yoshinari M, Aijima R, Murata N, Watanabe T, Oki Y, Kido MA
  • Journal Title: Nutrition Volume: 31 Issue: 2 Pages: 399-405
  • DOI: 10.1016/j.nut.2014.08.008
  • Peer Reviewed / Open Access
• [Presentation] ヒト口腔扁平上皮癌における温度感受性TRPチャネルの役割り (2017)
  • Author(s): 合島怜央奈， 山下佳雄，森 啓輔，靏岡祥子，檀上 敦，清島 保，城戸瑞穂
  • Organizer: 第35回日本口腔腫瘍学会総会・学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2017-01-26
• [Presentation] 新規KATP チャネル開口薬ZD0947 の平滑筋型KATP チャネルに対する効果 (2016)
  • Author(s): 森啓輔，合島怜央奈，檀上敦，山下佳雄
  • Organizer: 第58回歯科基礎医学会学術大会・総会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-08-24
• [Presentation] ヒト口腔癌細胞株におけるTRP チャネルの機能解析 (2016)
  • Author(s): 合島怜央奈，吉本怜子，曹愛琳，森啓輔，張旌旗，大崎康吉，檀上敦，山下佳雄，清島保，城戸瑞穂
  • Organizer: 第58回歯科基礎医学会学術大会・総会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-08-24
• [Presentation] TRPV4チャネルを介した温度変化による口腔上皮細胞調節 (2016)
  • Author(s): 吉本怜子，合島怜央奈，大崎康吉，曹愛琳，張旌旗，清島保，城戸瑞穂
  • Organizer: 第58回歯科基礎医学会学術大会・総会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-08-24
• [Presentation] プラズマ照射によるヒト口腔癌細胞株に対する抗腫瘍効果の検討 (2016)
  • Author(s): 合島怜央奈， 山下佳雄，靏岡祥子，下平大治，檀上 敦，後藤昌昭
  • Organizer: 第70回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-04-15
• [Presentation] 口腔創治癒過程における上皮間葉転換関連分子の発現変化 (2015)
  • Author(s): 合島怜央奈, 木附智子, 吉本怜子, 大崎康吉, 張 旌旗, 檀上 敦, 山下佳雄, 村田 祐造, 曹 愛琳, 城戸瑞穂
  • Organizer: 第9回三叉神経領域の感覚-運動統合機構研究会
  • Place of Presentation: 福岡市
  • Year and Date: 2015-11-28
• [Presentation] 温度感受性 TRPチャネルを介した口腔粘膜の創傷治癒制御 (2015)
  • Author(s): 合島怜央奈, 王 冰, 高尾知佳, 三原 弘, 加塩麻紀子, 大崎康吉, 張 旌旗, 木附智子, 村田 祐造, 富永真琴, 城戸瑞穂
  • Organizer: 生理学研究所研究会「生体ホメオスタシスのgatewayとしての上皮膜輸送マイクロホメオスタシス機構」
  • Place of Presentation: 岡崎市
  • Year and Date: 2015-09-15
  • Invited
• [Presentation] マウス口蓋創傷モデルの治癒過程における上皮間葉転換関連分子の発現解析 (2015)
  • Author(s): 合島怜央奈, 木附智子, 吉本怜子, 大崎康吉, 張 旌旗, 檀上 敦, 山下佳雄, 城戸瑞穂
  • Organizer: 第57回歯科基礎医学会学術大会・総会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-11