Pathogenic analysis of dipeptidyl-peptidase from Porphyromonas endodontalis

• Project/Area Number: 15H06505
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Nagasaki University
• Principal Investigator: NISHIMATA Haruka 長崎大学, 病院(歯学系), 医員 (10755755)
• Co-Investigator(Renkei-kenkyūsha): NEMOTO Takayuki K. 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (90164665)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯科 / 歯周病原細菌 / ポルフィロモナス属 / ジペプチジルペプチダーゼ / Porphyromonas属 / P. endodontalis

Outline of Final Research Achievements

The dental caries in infants progresses rapidly and pulpally, after it penetrates the protective enamel down to the dentin, in comparison with the same in adults. A periapical abscess occurs as the common clinical feature, when inflammation spreads to the pulp and the apical area of a deciduous tooth or an immature permanent tooth, secondary to the tooth decay. Porphyromonas endodontalis is the bacterium isolated from endodontic infections such as the root apex and the infected root canal involved with strong clinical manifestations.
Our study revealed that P. endodontalis and Porphyromonas gingivalis have dipeptidyl-peptidase (DPP)which decomposes extracellular oligopeptide into dipeptide as energy source. We found that each bacterium of these showed different activities of DPP.

Research Products

• [Presentation] PRG Barrier Coat Inhibits Biofilm Formation by Streptococcus mutans (2016)
  • Author(s): Haruka Nishimata, Yoko Kamasaki, Tomonori Hoshino, Taku Fujiwara
  • Organizer: 10th Biennial Conference of the Pediatric Dentistry Association of Asia
  • Place of Presentation: Tokyo Dome Hotel (東京都文京区）
  • Year and Date: 2016-05-27
  • Int'l Joint Research
• [Presentation] A Case with Disturbances in Tooth Formation caused by Therapy for Rhabdomyosarcoma (2016)
  • Author(s): Miyuki Nishiguchi, Keigo Imamura, Haruka Nishimata, Yoko Kamasaki, Kyoko Satoh, Yoshio Kondo, Naomi Tanoue, Taku Fujiwara
  • Organizer: 10th Biennial Conference of the Pediatric Dentistry Association of Asia
  • Place of Presentation: Tokyo Dome Hotel (東京都文京区）
  • Year and Date: 2016-05-27
  • Int'l Joint Research
• [Presentation] Construction of New Risk Predictive Model based on Machine Learning Method (2016)
  • Author(s): Yoshio Kondo, Yoichi Motomura, Keisuke Murayama, Haruka Nishimata, Kayo Nishida, Keiko Imamura, Kyoko Satoh, Kiyoshi Hidaka, Yoko Kamasaki, Miyuki Nishiguchi, Yomonori Hoshino, Kan Saito, Taku Fujiwara
  • Organizer: 10th Biennial Conference of the Pediatric Dentistry Association of Asia
  • Place of Presentation: Tokyo Dome Hotel (東京都文京区）
  • Year and Date: 2016-05-27
  • Int'l Joint Research
• [Presentation] PRG barrier coat inhibits biofilm formation by Streptococcus mutans (2016)
  • Author(s): Haruka Nishimata, Yoko Kamasaki, Tomonori Hoshino, and Taku Fujiwara
  • Organizer: 10th Conference of Pediatric Dentistry Association of Asia
  • Place of Presentation: 東京ドームホテル（東京都）
  • Year and Date: 2016-05-26
  • Int'l Joint Research
• [Presentation] 銀および同微粒子のStreptococcus mutansに対する抗菌効果の検討 (2016)
  • Author(s): 松崎拓海，西俣はるか，田上直美，藤原卓
  • Organizer: 第34回日本小児歯科学会九州地方会大会および総会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
• [Presentation] 骨形成不全症に伴う象牙質形成不全症乳歯の形態観察 (2016)
  • Author(s): 今村圭吾，釜﨑陽子，西俣はるか，近藤好夫，藤原卓
  • Organizer: 第34回日本小児歯科学会九州地方会および総会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）