Analysis of gene dynamics due to regulation of gene expression in embryonic inner ear

• Project/Area Number: 15H06511
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Kumamoto University
• Principal Investigator: Miwa Toru 熊本大学, 医学部附属病院, 非常勤診療医師 (70535591)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 内耳基礎 / 内耳発生 / 聴覚機能 / 蝸牛血管条 / 内耳特異的遺伝子ノックダウンマウス / 蝸牛電位 / 遺伝子導入 / 耳胞 / Dach1遺伝子 / 内耳形態 / 胎生期内耳

Outline of Final Research Achievements

We performed transuterine Dach1 targeted shRNA(shRNA-Dach1) gene transfer into the otocyst of wild-type mice to generate the inner ear specific Dach1 knockdown mice. Reporter gene (eGFP) expressed in the treated ear at embryonic day 15 (E15) and E18. No expression of Dach1 mRNA observed in the treated inner ear at E15 and E18. Pax6 and Igf1 mRNA expression reduced in the treated side at E15.
At postnatal day 30 (P30), stria vascularis was thinned in the shRNA-Dach1 knockdown mice compared to wild-type mice.