| Project/Area Number |
15H06600
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-08-28 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | ドコサヘキサエン酸 / リン脂質 / 後期エンドソーム / BMP / 脂質 / DHA / DPAn-6 |
|---|
| Outline of Final Research Achievements |
BMP is an enigmatic phospholipid that is specifically localized in intralumenal vesicles of late endosomes, and is thought to be essential for proper functions of late endosomes. BMP has a propensity to incorporate an omega-3 fatty acid DHA, and DHA is highly enriched in BMP. However, the physiological meaning of this DHA-containing BMP fatty acyl species is unknown, mainly due to the fact that biosynthetic enzymes of BMP have not been identified so far.
A non-targeted lipidomic analysis on knockout mouse organs of a lipid metabolizing enzyme recently identified a sought-after enzyme for the biosynthesis of BMP. The knockout organs exhibited reduced levels of DHA-containing BMP species. Furthermore, in testes from a recently generated PUFA-deficient mouse model, DPAn-6, which usually constitutes more than 70% of fatty acids in BMP, was nearly depleted. These mouse models seem to be very useful to reveal physiological roles of the unique fatty acyl composition of BMP.
|
