Protective role of leukotriene B4 receptor type 2 in acute lung injury

• Project/Area Number: 15H06604
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology
• Research Institution: Juntendo University
• Principal Investigator: Shigematsu Misako 順天堂大学, 医学(系)研究科(研究院), 非常勤助手 (60760303)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 急性肺傷害 / 脂質受容体 / ロイコトリエン / ニューモライシン / モンテルカスト / 脂質 / 感染症

Outline of Final Research Achievements

In this study we explored the roles of leukotriene B4 receptor type 2 (BLT2) in the lung using an acute lung injury model induced by a pneumococcal toxin, pneumolysin (PLY).
BLT2 signaling plays important role in protecting against the PLY-induced acute lung injury by suppressing cysteinyl leukotrienes receptor (CysLT1) expression in lung endothelial cells, and by attenuating the susceptibility to PLY-dependently produced cysteinyl leukotrienes. Additionally, CysLT1 antagonists that currently used as anti-asthmatic drugs will be beneficial to treat acute lung injury in the early phase of pneumococcal pneumonia.

Research Products

• [Int'l Joint Research] ジョージア大学(米国)
• [Int'l Joint Research] ジュネーヴ大学(スイス)
• [Journal Article] Leukotriene B4 receptor type 2 protects against pneumolysin-dependent acute lung injury (2016)
  • Author(s): Shigematsu, M., Koga, T., Ishimori, A., Saeki, K., Ishii, Y., Taketomi, Y., Ohba, M., Jo-Watanabe, A., Okuno, T., Harada, N., Harayama, T., Shindou, H., Li, J. D., Murakami, M., Hoka, S.,Yokomizo, T.
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 34560-34560
  • DOI: 10.1038/srep34560
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 12-HHT/BLT2 axis protects against pneumolysin-dependent acute lung injury (2017)
  • Author(s): Kazuko Saeki, Misako Shigematsu, Tomoaki Koga, Mai Ohba, Toshiaki Okuno, Takehiko Yokomizo
  • Organizer: Keystone Symposium, Integrating Metabolism and Immunity
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2017-05-29
  • Int'l Joint Research
• [Presentation] Protective roles of 12-hydroxyheptadecatrienoic acid/BLT2 in acute lung injury (2017)
  • Author(s): Misako Shigematsu, Tomoaki Koga, Kazuko Saeki, Mai Ohba, Toshiaki Okuno, Takehiko Yokomizo
  • Organizer: Keystone Symposium, Lipidomics and Bioactive Lipids in Metabolism and Disease
  • Place of Presentation: Granlibakken Tahoe, USA
  • Year and Date: 2017-02-26
  • Int'l Joint Research
• [Presentation] The roles of 12-hydroxyheptadecatrienoic acid/BLT2 axis in skin (2016)
  • Author(s): Misako Shigematsu, Tomoaki Koga, Kazuko Saeki, Toshiaki Okuno, Takehiko Yokomizo
  • Organizer: International Conference on the Bioscience of Lipids
  • Place of Presentation: Chamonix-Mont-Blanc, France
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] 12-hydroxyheptadecatrienoic acid (12-HHT) as a novel lipid mediator (2016)
  • Author(s): Takehiko Yokomizo, Yumiko Ishii, Misako Shigematsu, Kazuko Saeki, Toshiaki Okuno
  • Organizer: 日本脂質生化学会
  • Place of Presentation: 秋田
  • Year and Date: 2016-06-09
  • Invited
• [Presentation] BLT2 receptor protects against lethal acute lung injury induced by a pneumococcal toxin pneumolysin (2016)
  • Author(s): Misako Shigematsu, Tomoaki Koga, Kazuko Saeki, Toshiaki Okuno, Takehiko Yokomizo
  • Organizer: Experimental Biology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-04-02
  • Int'l Joint Research
• [Presentation] ロイコトリエンB4第二受容体BLT2は急性肺傷害時に肺保護作用を発揮する (2015)
  • Author(s): 重松美沙子、古賀友紹、佐伯和子、奥野利明、外須美夫、横溝岳彦
  • Organizer: 第35回日本分子生物学会年会・第38回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] Pneumolysin誘導性急性肺傷害におけるBLT2の肺保護作用 (2015)
  • Author(s): 重松美沙子、古賀友紹、佐伯和子、奥野利明、横溝岳彦
  • Organizer: 第12回東京呼吸器リサーチフォーラム
  • Place of Presentation: 帝人株式会社グループ 東京本社
  • Year and Date: 2015-11-14
• [Remarks] 順天堂大学医学部 生化学第一講座 ホームページ
  • URL: http://plaza.umin.ac.jp/j_bio/Biochem1/Top.html