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Ultra-thin film wrinkle assay to visualize and evaluate the cell traction force

Research Project

Project/Area Number 15H06627
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokai University

Principal Investigator

Yokoyama Sho  東海大学, マイクロ・ナノ研究開発センター, 特定研究員 (30760425)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsTraction force / TFM / Wrinkle / Contractile / Drug efficacy evaluation / Contractile force / トラクションフォース / トラクションフォースマイ クロスコピー / 超薄膜 / ナノ材料 / 生物・生体工学
Outline of Final Research Achievements

The experimental method of measuring cell traction force (the force of cells to pull extracellular matrix and another cells) is called traction force microscopy (TFM). The dynamic mechanical behavior of cell-ECM and cell-cell interactions is known to influence a vast range of cellular functions, including necrosis, differentiation, adhesion, migration, locomotion, and growth. TFM can clarify the unique functions and properties of cells, contribute to the development of cell biology, qualitatively evaluate the response of cells to chemicals, and be applied to large-scale screening work for drug discovery.
In this research, we aimed for superior TFM than the conventional method and developed a novel cell traction force evaluation method using "wrinkle" generated on gold ultra-thin film. We realized visualization of the size and direction of the cell traction force and carried out basic analysis to quantify the cell traction force.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report

Research Products

(20 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (17 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] New wrinkling substrate assay reveals traction force fields of leader and follower cells undergoing collective migration2017

    • Author(s)
      Yokoyama, S., Matsui, T.S., Deguchi, S.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 482 Pages: 975-979

    • DOI

      10.1016/j.bbrc.2016.11.142

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Microcontact peeling: A cell micropatterning technique for circumventing direct adsorption of proteins to hydrophobic PDMS.2017

    • Author(s)
      S. Yokoyama, T. S. Matsui, S. Deguchi
    • Journal Title

      Current Protocols in Cell Biology

      Volume: 75

    • DOI

      10.1002/cpcb.22

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of a novel device with high throughout axonal transport quantification2016

    • Author(s)
      R. Araki, A. Otomo, J. Wada, T. Ishida, S. Yokoyama, S. Hadano, H. Kimura
    • Journal Title

      Proceedings of the School of Engineering of Tokai University

      Volume: 30 Pages: 67-73

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Local geometry sensing by individual focal adhesions2017

    • Author(s)
      S. Deguchi, S. Yokoyama, T. S. Matsui, T. Ohnishi
    • Organizer
      2017 Cellular and Molecular Bioengineering Annual Conference
    • Place of Presentation
      Hapuna Beach Prince Hotel(Big Island of Hawaii, USA)
    • Year and Date
      2017-01-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 軸索小胞輸送の定量評価に向けた開放型マイクロデバイスの開発および評価2017

    • Author(s)
      横山奨, 串田隆志, 石田智之, 大友麻子, 秦野伸二, 木村啓志
    • Organizer
      シンポジウム:細胞アッセイ技術と現状と将来
    • Place of Presentation
      東京大学生産技術研究所(東京都目黒区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイクロ流体デバイスを用いた血栓クリーナーの定量的機能評価2017

    • Author(s)
      横山奨, 吉田翔太, 岡村陽介, 木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会第8回学術講演会
    • Place of Presentation
      東海大学湘南キャンパス(神奈川県平塚市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 神経細胞軸索内小胞動態解析の効率化に向けたマイクロデバイスの開発2017

    • Author(s)
      串田隆志, 石田智之, 横山奨, 大友麻子, 秦野伸二, 木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会第8回学術講演会
    • Place of Presentation
      東海大学湘南キャンパス(神奈川県平塚市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 神経細胞軸索内小胞動態解析の効率化に向けたマイクロデバイスの開発2017

    • Author(s)
      串田隆志, 横山奨, 大友麻子, 秦野伸二, 木村啓志
    • Organizer
      関東学生会第56回学生員卒業研究発表講演会
    • Place of Presentation
      東京理科大学葛飾キャンパス(東京都葛飾区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイクロ流体デバイスを用いたALS疾患神経細胞の軸策輸送動態の解析2017

    • Author(s)
      石田智之, 大友麻子, 杉山純也, 横山奨, 串田隆志, 秦野伸二, 木村啓志
    • Organizer
      関東学生会第56回学生員卒業研究発表講演会
    • Place of Presentation
      東京理科大学葛飾キャンパス(東京都葛飾区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイクロデバイスを用いた軸索輸送現象イメージング2016

    • Author(s)
      横山奨, 串田隆志, 石田智之, 大友麻子, 秦野伸二, 木村啓志
    • Organizer
      第八回「光塾」
    • Place of Presentation
      東京工業大学すずかけ台キャンパス(神奈川県横浜市)
    • Year and Date
      2016-12-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイクロ流体デバイスを用いたALS疾患モデルの解析2016

    • Author(s)
      石田智之, 荒木良介, 串田隆志, 杉山純也, 大友麻子, 横山奨, 秦野伸二, 木村啓志
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] New traction force microscopy suggests a mechanism of local geometry sensing by individual cell adhesions2016

    • Author(s)
      S. Deguchi, S. Yokoyama, T. S. Matsui, T. Ohnishi
    • Organizer
      International Conference on Flow Dynamics 2016
    • Place of Presentation
      Sendai International Center(Sendai, Japan)
    • Year and Date
      2016-10-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of a novel device with high-throughout axonal transport quantification2016

    • Author(s)
      S. Yokoyama, J. Wada, T. Kushida, R. Araki, A. Otomo, S. Hadano, H. Kimura
    • Organizer
      The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Convention Center Dublin(Dublin, Ireland)
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 超薄膜を用いた細胞収縮力の可視化と定量化への試み2016

    • Author(s)
      横山奨, 八十田穣, 槌谷和義, 木村啓志
    • Organizer
      2016年度日本機械学会年次大会
    • Place of Presentation
      九州大学伊都キャンパス(福岡県福岡市)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] ルードヴィッヒ・ソレー効果による重水-軽水分離のためのマイクロ流体デバイスの開発2016

    • Author(s)
      諸星和, 中山耕史朗, 江口和也, 横山奨, 槌谷和義, 喜多理王, 木村啓志
    • Organizer
      2016年度日本機械学会年次大会
    • Place of Presentation
      九州大学伊都キャンパス(福岡県福岡市)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] 金超薄膜を用いた細胞トラクションフォース可視化技術の開発2016

    • Author(s)
      横山奨, 八十田穣, 槌谷和義, 木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会第7回学術講演会
    • Place of Presentation
      東海大学湘南キャンパス(神奈川県平塚市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイクロ流体デバイスを用いたALS疾患モデルの解析2016

    • Author(s)
      石田智之, 大友麻子, 横山奨, 串田隆志, 秦野伸二, 木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会第7回学術講演会
    • Place of Presentation
      東海大学湘南キャンパス(神奈川県平塚市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 神経細胞軸索内小胞動態解析の効率化に向けたアッセイデバイスの開発 -微小スリット作製条件の最適化検討-2016

    • Author(s)
      串田隆志, 横山奨, 大友麻子, 秦野伸二, 木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会第7回学術講演会
    • Place of Presentation
      東海大学湘南キャンパス(神奈川県平塚市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 神経細胞軸索内小胞態の効率的定量評価アッセイデバイスの開発2016

    • Author(s)
      横山奨, 串田隆志, 石田智之, 大友麻子, 秦野伸二, 木村啓
    • Organizer
      2016年度第12回総合医学研究所研修会
    • Place of Presentation
      東海大学伊勢原キャンパス(神奈川県伊勢原市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞収縮能評価方法の開発とその応用2015

    • Author(s)
      横山奨
    • Organizer
      日本機械学会2015年度年次大会
    • Place of Presentation
      北海道大学工学部(北海道)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report

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Published: 2015-08-26   Modified: 2018-03-22  

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