| Project/Area Number |
15H06657
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Animal production science
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| Research Institution | Hoshi University |
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Principal Investigator |
YAMAMOTO Naoki 星薬科大学, 先端生命科学研究所, 特任助教 (50757432)
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| Research Collaborator |
KINOSHITA Tetsu
HATADA Izuho
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | エピゲノム編集 / DNAメチル化 / エピジェネティック / CRISPR/Cas9 / 発現制御 / 発生・分化 / インプリンティング / long non-coding RNA |
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| Outline of Final Research Achievements |
This study aims at establishing a technology that can specifically control the DNA methylation status of a specific genome region . A method using forced transcription of long noncoding RNA (200 bases or more), which do not translated into a protein, failed to obtain sufficient transcription efficiency and the expected effect was not obtained. On the other hand, using an artificial fusion gene obtained by fusing a DNA binding domain called dead Cas 9, which can be customized to recognize an arbitrary DNA sequence, and DNA-methylation-modifiing enzyme domain, I succeed to manipulate DNA methylation levels of a specific genome region with very high efficiency. The technology developed in this research is expected to greatly advance DNA methylation research over a wide range from basic to clinical research.
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