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Development of a visualization method by using HCR-FISH and Click Chemistry

Research Project

Project/Area Number 15H06814
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Civil and environmental engineering
Research InstitutionMatsue National College of Technology

Principal Investigator

Yamaguchi Tsuyoshi  松江工業高等専門学校, 環境・建設工学科, 助教 (30759832)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords高感度FISH法 / HCR法 / click chemistry / Click chemistry / FISH法 / 環境微生物 / 微生物 / 視覚的検出 / 原核生物
Outline of Final Research Achievements

In this study, combination with in situ DNA-hybridization chain reaction (HCR) and click chemistry was developed for successfully detecting microorganisms in oligotrophic. Although It high specificity by using Escherichia coli was revealed, click chemistry was not successful. It may be applicable for detection of an environmental microorganism after optimization of click chemistry in situ.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (9 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Rapid and Sensitive Identification of Marine Bacteria by an Improved in situ DNA-Hybridization Chain Reaction (quickHCR-FISH)2015

    • Author(s)
      Tsuyoshi Yamaguchi, Bernhard M. Fuchs, Rudolf Amann, Shuji Kawakami, Kengo Kubota, Masashi Hatamoto, Takashi Yamaguchi.
    • Journal Title

      Systematic Applied MIcrobiology

      Volume: 38 Pages: 400-405

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 新規高感度FISH法におけるhzo遺伝子を標的としたプローブの選定2017

    • Author(s)
      大野裕之, 武邊勝道, 山口剛士
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 中海に生息するアナモックス細菌の微生物群集構造解析2017

    • Author(s)
      橋田一輝, 山口剛士, 武邊勝道, 加藤季晋
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Click chemistryを利用した高感度FISH法の開発2017

    • Author(s)
      木村圭祐, 川上周司, 山口隆司, 山口剛士
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of a novel sensitive FISH by using click chemistry2017

    • Author(s)
      Hiroyuki Oono, Keisuke Kimura, Tsuyoshi Yamaguchi and Takashi Yamaguchi
    • Organizer
      STI-Gigaku2017
    • Place of Presentation
      長岡
    • Year and Date
      2017-01-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A new sensitive FISH: Fluorescence amplification using click chemistry for detection of microorganisms2016

    • Author(s)
      Keisuke Kimura, Tsuyoshi Yamaguchi, Katsumichi Takebe and Takashi Yamaguchi
    • Organizer
      The 4th Gigaku conference
    • Place of Presentation
      長岡
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A rapid in situ DNA-hybridization chain reaction protocol (quickHCR-FISH) for detection of marine bacteria2016

    • Author(s)
      Tsuyoshi Yamaguchi, Bernhard M. Fuchs, Rudolf Amann, Shuji Kawakami, Kengo Kubota, Masashi Hatamoto and Takashi Yamaguchi.
    • Organizer
      16 th International Symposium on Microbial Ecology (ISME16)
    • Place of Presentation
      カナダ
    • Year and Date
      2016-08-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 宍道湖における主要微生物の生息状況と水質との相関の考察2016

    • Author(s)
      庄司和希, 山口剛士, 川上周司, 嘉藤健二, 崎幸子, 山口隆司
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      徳島県
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] Click chemistryを用いた新規高感度FISH法の開発2016

    • Author(s)
      木村圭佑, 山口剛士, 川上周司, 山口隆司
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      徳島県
    • Year and Date
      2016-03-16
    • Related Report
      2015 Annual Research Report

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Published: 2015-08-26   Modified: 2018-03-22  

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