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Study on mechanisms of kleptochloroplast enlargement and division in the dinoflagellate Nusuttodinium

Research Project

Project/Area Number 15H06834
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Evolutionary biology
Research InstitutionNational Institute of Genetics

Principal Investigator

Onuma Ryo  国立遺伝学研究所, 細胞遺伝研究系, 博士研究員 (80756825)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords盗葉緑体 / 細胞内共生 / 渦鞭毛藻 / クリプト藻 / Nusuttodinium
Outline of Final Research Achievements

Dinoflagellate Nusuttodinium aeruginosum possesses kleptochloroplast stolen from cryptomonad. To investigate molecular mechanism of kleptochloroplastidy, we compared transcriptomes of cryptomonad as free-living cell and as ingested form. The analysis showed that the ingested nucleus remained active and suggested that the activities of photosynthesis and nitrogen assimilation were retained. In contrast, expressions of cell cycle-related genes were decreased, implying that the cell cycle of the ingested cryptomonad was not progressed.
In addition, prior to study on mechanism of kleptochloroplast division, we investigate mechanisms of regulation of chloroplast division in cryptophyte Guillardia theta. Nucleomorph-encoded genes/proteins that are related to nucleomorph replication and chloroplast division were constantly expressed throughout the cell cycle, suggesting that the timing of chloroplast division is regulated by as-yet-unidentified gene/protein that encoded in the nucleus.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (4 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta2017

    • Author(s)
      Ryo Onuma, Neha Mishra, Shin-ya Miyagishima
    • Journal Title

      Scientific Reports

      Volume: In Press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 盗葉緑体性渦鞭毛藻Nusuttodinium aeruginosumに取り込まれた共生クリプト藻の網羅的発現遺伝子解析2017

    • Author(s)
      大沼亮、廣岡俊亮、兼崎友、吉川博文、宮城島進也
    • Organizer
      日本藻類学会第41回大会
    • Place of Presentation
      高知大学、高知市
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Ultrastructural and transcriptomic studies of kleptochloroplastidic dinoflagellate Nusuttodinium aeruginosum (Dinophyceae)2016

    • Author(s)
      Ryo Onuma, Takeo Horiguchi, Shin-ya Miyagishima
    • Organizer
      Protist 2016 Moscow Forum
    • Place of Presentation
      Moscow State University, Moscow, Russia
    • Year and Date
      2016-06-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] クリプト藻Guillardia thetaの葉緑体分裂と共生藻細胞周期による分裂制御機構の解明2016

    • Author(s)
      大沼亮、宮城島進也
    • Organizer
      日本藻類学会第40回大会
    • Place of Presentation
      日本歯科大学
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report

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Published: 2015-08-26   Modified: 2018-03-22  

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