Development of automated data collection and processing system for multiple microcrystals

• Project/Area Number: 15H06862
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Yamashita Keitaro 国立研究開発法人理化学研究所, 放射光科学総合研究センター, 基礎科学特別研究員 (20721690)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: X線結晶解析 / 構造生物学

Outline of Final Research Achievements

We developed an automated data collection and processing system for multiple microcrystals to allow structure analyses of challenging targets that do not produce crystals large enough to collect complete data from single crystals. The workflow of data collection and processing for microcrystals was first established and automated. The system was tested using various protein samples obtained via collaborations. The data collection and processing, and generation of graphical report were automated. This system dramatically reduced time and human effort that spent after crystallization.

Research Products

• [Journal Article] Crystal Engineering of Self-Assembled Porous Protein Materials in Living Cells (2017)
  • Author(s): S. Abe, H. Tabe, H. Ijiri, K. Yamashita, K. Hirata, K. Atsumi, T. Shimoi, M. Akai, H. Mori, S. Kitagawa and T. Ueno
  • Journal Title: ACS Nano Volume: 11 Issue: 3 Pages: 2410-2419
  • DOI: 10.1021/acsnano.6b06099
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] PXビームラインにおける回折データ自動処理・構造決定システムの開発 (2017)
  • Author(s): 山下恵太郎，平田邦生，河野能顕，上野剛，長谷川和也，熊坂崇，山本雅貴
  • Organizer: 第30回放射光学会年会
  • Place of Presentation: 神戸芸術センター（兵庫県神戸市）
  • Year and Date: 2017-01-07
• [Presentation] 回折データ自動収集・処理システムZooによる微小結晶を用いた高難度タンパク質構造解析の効率化 (2016)
  • Author(s): 山下恵太郎，平田邦生，河野能顕，上野剛，長谷川和也，熊坂崇，山本雅貴
  • Organizer: 第16回日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-06-07
• [Presentation] 微小結晶のための回折データ自動処理システムの開発 (2016)
  • Author(s): 山下恵太郎，平田邦生，河野能顕，上野剛，長谷川和也，熊坂崇，山本雅貴
  • Organizer: 第29回放射光学会年会
  • Place of Presentation: 東京大学
  • Year and Date: 2016-01-09