| Project/Area Number |
15H06906
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
Ishii Shunichi 国立研究開発法人海洋研究開発機構, 海底資源研究開発センター, 研究員 (10556913)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 微生物電解セル / 菌体外電子移動 / バイオカソード / 微生物電気合成 / 電極酸化メタン菌 / 微生物群集構造解析 / メタゲノム / メタン生成 / Methanobacterium / Geoalkalibacter / 電気合成 / 二酸化炭素固定 |
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| Outline of Final Research Achievements |
In this study, we operated a microbial electrolysis cell (MEC) for the stimulation and acceleration of microbial methanogenesis via voltage input to fix CO2 to valuable compounds such as methane. A MEC system was operated for 11 months where 600 mV of voltage was applied between anode and cathode, resulting in a successful conversion of CO2 to methane with electrical current consumption. The 16S rRNA-based community analyses revealed that genera Methanocalculus and Methanobacterium were located in the electromethanogenic cathode, while genus Geoalkalibacter was shown in the electrode-respiring anode. The metagenomic analysis revealed that the Geoalkalibacter genome coded numerous numbers of multi-heme c-type cytochromes, whereas methanogenic archaeal genomes contained many hydrogenases. These results suggest that direct electron transfer via cytochromes occurred on the anode, and hydrogen-mediated electron transfer functioned on the cathode.
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