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抗菌性と骨適合性の両方に優れる溶解性制御型Ag添加リン酸カルシウム膜の作製

Research Project

Project/Area Number 15J00660
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Composite materials/Surface and interface engineering
Research InstitutionTohoku University

Principal Investigator

GOKCEKAYA OZKAN  東北大学, 工学研究科, 特別研究員(PD)

Project Period (FY) 2015-04-24 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,170,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsリン酸カルシウム / Ag / 抗菌性 / コーティング / HRTEM / 細胞毒性 / silver / incorporation / antibacterial / calcium phosphate / hydroxyapatite / tricalcium phosphate / cytotoxiciy
Outline of Annual Research Achievements

I. Ag含有リン酸カルシウム粉末の抗菌性評価
湿式法によりAg含有リン酸カルシウム粉末を合成した。Agの溶解度はハイドロキシアパタイト(HA)よりもβ型リン酸三カルシウム(β-TCP)の方が大きいことを見出した。Ag固溶β-TCPはβ-TCPの溶解に伴いAgイオンを放出することから、同量のAgを含有する金属Ag含有HAよりも擬似体液中へのAg放出量が大きく、長期間の放出を維持することを明らかにした。それに伴い、Ag固溶β-TCPは大腸菌に対する抗菌性を示した。一方、ISO 10993-5に準拠したV79細胞を用いた毒性評価試験を行い、Ag固溶β-TCPは細胞毒性は示さないことを確認した。
II. Ag固溶β型リン酸三カルシウムの構造解析
β-TCP中においてAgはCaサイトに置換することが知られている。本研究では、β-TCP中のAgの位置を明らかにするために、高分解能透過型電子顕微鏡(HRTEM)の使用方法を習得し、Ag固溶β-TCPを観察した。Agはβ-TCPのCa(4)サイトに存在することを明らかにした。これはシミュレーションの結果とも一致していた。
III. Ag含有非晶質リン酸カルシウム薄膜の抗菌性評価
RFマグネトロンスパッタリング法により、Ag含有非晶質リン酸カルシウム(AgACP)薄膜をTi基板上に作製した。擬似体液への溶解性試験の結果、ACP薄膜の溶解に伴いAgも放出されることを明らかにした。膜厚0.5μmのAgACP薄膜のシェーク法による抗菌性評価方法を確立し、大腸菌および黄色ブドウ球菌のいずれに対しても抗菌性を発現することを明らかにした。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (17 results)

All 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Int'l Joint Research: 6 results,  Invited: 2 results)

  • [Journal Article] In vitro evaluation of Ag-containing calcium phosphates: Effectiveness of Ag-incorporated β-tricalcium phosphate2017

    • Author(s)
      O. Gokcekaya, K. Ueda, K. Ogasawara, H. Kanetaka, T. Narushima
    • Journal Title

      Mater. Sci. Eng. C

      Volume: 75 Pages: 926-933

    • DOI

      10.1016/j.msec.2017.02.059

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro performance of Ag-incorporated hydroxyapatite and its adhesive porous coatings deposited by electrostatic spraying2017

    • Author(s)
      Ozkan Gokcekaya, Thomas J. Webster, Kyosuke Ueda, Takayuki Narushima, Celaletdin Ergun
    • Journal Title

      Mater. Sci. Eng. C

      Volume: 75 Pages: 556-564

    • DOI

      10.1016/j.msec.2017.03.233

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ceramic coating of Ti and its alloys using dry processes for biomedical applications2016

    • Author(s)
      Ueda T., Kondo N., Sado S., Gokcekaya O., Ueda K., Ogasawara K. and Narushima T.
    • Journal Title

      Interface Oral Health Science 2016: Innovative Research on Biosis & Abiosis Intelligent Interface

      Volume: - Pages: 23-34

    • DOI

      10.1007/978-981-10-1560-1_2

    • ISBN
      9789811015595, 9789811015601
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro properties of Ag-containing calcium phosphates2016

    • Author(s)
      O. Gokcekaya, K. Ueda, T. Narushima, K. Ogasawara, H. Kanetaka
    • Journal Title

      40th Int'l Conf & Expo on Advanced Ceramics & Composites, Next Generation Bioceramics and Biocomposites

      Volume: -

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Synthesis and characterization of Ag-containing calcium phosphates with various Ca/P ratios2015

    • Author(s)
      O. Gokcekaya, K. Ueda, T. Narushima, C. Ergun
    • Journal Title

      Mater. Sci. Eng. C

      Volume: 53 Pages: 111-119

    • DOI

      10.1016/j.msec.2015.04.025

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Control of Ag release from Ag-containing calcium phosphates in simulated body fluid2015

    • Author(s)
      O. Gokcekaya, K. Ueda, T. Narushima
    • Journal Title

      Ceramics Transactions

      Volume: 254 Pages: 13-20

    • DOI

      10.1002/9781119190134.ch2

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro evaluation of Ag-containing calcium phosphates2015

    • Author(s)
      O. Gokcekaya, K. Ueda, T. Narushima, K. Ogasawara
    • Journal Title

      Twenty-Fourth International Symposium on Processing and Fabrication of Advanced Materials Proceedings

      Volume: - Pages: 95-103

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Ti上に作製されたAg含有非晶質リン酸カルシウム膜の評価2017

    • Author(s)
      井上紅花、上田恭介、O. Gokcekaya、伊藤甲雄、小笠原康悦、成島尚之
    • Organizer
      日本金属学会第160回大会(2017年春期)
    • Place of Presentation
      首都大学東京, 八王子市
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Fabrication of a bioresorbable Ag-containing amorphous calcium phosphate coating film and evaluation of its antibacterial activity2016

    • Author(s)
      K. Ueda, N. Kondo, O. Gokcekaya, T. Kasuga, A. Obata, K. Ogasawara, H. Kanetaka, T. Narushima
    • Organizer
      The 9th Pacific Rim International Conference on Advanced Materials and Processing (PRICM9)
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Year and Date
      2016-08-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In vitro dissolution behavior and antibacterial activity of Ag-containing biphasic calcium phosphates2016

    • Author(s)
      O. Gokcekaya, K. Ueda, T. Narushima, K. Ogasawara, H. Kanetaka
    • Organizer
      The 9th Pacific Rim International Conference on Advanced Materials and Processing (PRICM9)
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Year and Date
      2016-08-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of chemical state of silver added to calcium phosphates on dissolution behavior, antibacterial activity, and cytotoxicity2016

    • Author(s)
      K. Ueda, O. Gokcekaya, K. Ogasawara, H. Kanetaka, T. Narushima
    • Organizer
      International Conference on Processing & Manufacturing of Advanced Materials (THERMEC’ 2016)
    • Place of Presentation
      MESSE GRAZ, Graz, Austria
    • Year and Date
      2016-05-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] TiへのAg含有非晶質リン酸カルシウム薄膜の作製とその抗菌性評価2016

    • Author(s)
      上田恭介、近藤なつ美、O. Gokcekaya、小幡亜希子、春日敏宏、小笠原康悦、成島尚之
    • Organizer
      軽金属学会第130回春期大会
    • Place of Presentation
      大阪大学, 吹田市
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] In vitro properties of Ag-containing calcium phosphates2016

    • Author(s)
      O. Gokcekaya, K. Ueda, T. Narushima, K. Ogasawara, H. Kanetaka
    • Organizer
      40th Int'l Conf & Expo on Advanced Ceramics & Composites
    • Place of Presentation
      Hilton Daytona Beach Resort and Ocean Center, Daytona Beach, Florida, USA
    • Year and Date
      2016-01-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In vitro evaluation of Ag-containing calcium phosphates2015

    • Author(s)
      O. Gokcekaya, K. Ueda, T. Narushima, K. Ogasawara
    • Organizer
      Twenty-Fourth International Symposium on Processing and Fabrication of Advanced Materials
    • Place of Presentation
      Centenary Memorial Hall, Senriyama Campus, Kansai University, Osaka, Japan
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Antibacterial activity and cytotoxicity of Ag-containing calcium phosphates2015

    • Author(s)
      Ozkan Gokcekaya、上田恭介、成島尚之、小笠原康悦、金髙弘恭
    • Organizer
      日本金属学会 第157回大会(2015年秋期)
    • Place of Presentation
      九州大学, 福岡市
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evaluation of antibacterial activity of Ag-containing calcium phosphates2015

    • Author(s)
      K. Ueda, O. Gokcekaya, T. Narushima, K. Ogasawara, H. Kanetaka
    • Organizer
      The 10th Anniversary International Workshop on Biomaterials in Interface Science
    • Place of Presentation
      Baden-ya Soho, Zao
    • Year and Date
      2015-08-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ag含有リン酸カルシウムの抗菌性評価2015

    • Author(s)
      上田恭介、関山雅人、Ozkan Gokcekaya、成島尚之、小笠原康悦
    • Organizer
      粉体粉末冶金協会平成27年度春季大会
    • Place of Presentation
      早稲田大学国際会議場, 新宿区
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report

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Published: 2015-11-26   Modified: 2024-03-26  

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