Insight into the Adaptive Evolution and Diversification in Erpobdelliformes

• Project/Area Number: 15J00720
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 国内
• Research Field: Biodiversity/Systematics
• Research Institution: Hiroshima University
• Principal Investigator: 中野 隆文 広島大学, 教育学研究科, 特別研究員(PD)
• Project Period (FY): 2015-04-24 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 系統分類 / ヒル類 / 分子系統 / アジア / 新規形質 / 再検討 / 東南アジア / 新種

Outline of Annual Research Achievements

平成29年度は3年間の研究課題のまとめとして，これまでに得られた研究成果の論文化を行った．加えて捕食性ヒル類にみられる特殊な形態形質「胃通管」の機能を明らかにした．
先ず，東南アジア産イシビル形亜目捕食性ヒル類の分類学的位置，ならびにそれらの系統関係を推定し，本亜目における進化史の再推定を行った論文を国際学術雑誌発表した．同論文では，分子系統解析の結果に基づき，東南アジアに分布するイツウコウビル類は，胃通管を有する系統と，胃通管をもたない系統に分かれることを明らかにした．さらにイツウコウビル類は全北区に広く分布する淡水棲捕食性ヒル類のイシビル科と姉妹群になるという結果が得られた．
胃通管については，東アジアに生息するクガビル類の胃通管が「二次的生殖器官」であることを初めて明らかにした．胃通管は胃に接続する管状構造で，その管は腹側に開口するのだが，その機能は長らく不明で，「二次的な肛門」あるいは「二次的な生殖器官」であろうと考えられてきた．四国より得られたクガビル類の1未記載種の胃通管内に半透明のカプセルが詰まっていることを発見した．したがって，そのカプセルが何であるかを特定するために，透過型電子顕微鏡（TEM）によるカプセル内容物の微細構造観察を実施した．TEM観察したところ，ヒル類の精子に特徴的な形質状態が認められたため，得られたカプセルはクガビル類の「精包」であると断定できた．したがって，クガビル類の「胃通管」は「二次的生殖器官」であると結論づけ，国際学術雑誌にその研究成果を発表した．ヒル類の生殖様式における祖先形質状態は，「精包」を皮膚に植え付ける様式で，これら生殖様式は淡水棲，海産のグループにおいて見られる．しかしながら，クガビル類は陸棲の分類群であるため，クガビル類における胃通管は精包を受け取るために創出された新規形質ではないかと，同論文で議論した．

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] マヒドン大学/チュラーロンコーン大学(タイ)
• [Int'l Joint Research] 国立台湾大学(台湾)
• [Int'l Joint Research] ベトナム国立自然博物館/生態生物資源研究所(ベトナム)
• [Int'l Joint Research] マヒドン大学/チュラーロンコーン大学(タイ)
• [Journal Article] A new species of the genus Pseudocrangonyx (Crustacea, Amphipoda, Pseudocrangonyctidae) from Korea (2018)
  • Author(s): Chi-Woo Lee, Ko Tomikawa, Takafumi Nakano, Gi-Sik Min
  • Journal Title: ZooKeys Volume: 735 Pages: 27-44
  • DOI: 10.3897/zookeys.735.21697
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Systematic revision of the Southeast Asian macrophagous leeches, with the description of two new gastrostomobdellid species (Hirudinida: Arhynchobdellida: Erpobdelliformes) (2018)
  • Author(s): Takafumi Nakano, Koshiro Eto, Kanto Nishikawa, Mohamad Yazid Hossman, Ekgachai Jeratthitikul
  • Journal Title: Zoological Journal of the Linnean Society Volume: 印刷中 Issue: 1 Pages: 1-30
  • DOI: 10.1093/zoolinnean/zlx097
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A new landhopper species of Myanmarorchestia (Crustacea: Amphipoda: Talitridae) from Yunnan, China (2018)
  • Author(s): Takafumi Nakano, Ko Tomikawa, Zhone Hou, Hiroshi Morino
  • Journal Title: Journal of Natural History Volume: 52 Issue: 17-18 Pages: 1181-1199
  • DOI: 10.1080/00222933.2018.1453098
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Two new subterranean species of Pseudocrangonyx Akatsuka & Komai, 1922 (Amphipoda: Crangonyctoidea: Pseudocrangonyctidae), with an insight into groundwater faunal relationships in western Japan (2018)
  • Author(s): Ko Tomikawa, Takafumi Nakano
  • Journal Title: Journal of Crustacean Biology Volume: 印刷中 Issue: 4 Pages: 460-474
  • DOI: 10.1093/jcbiol/ruy031
  • Peer Reviewed
• [Journal Article] A new quadrannulate species of Orobdella (Hirudinida: Arhynchobdellida: Orobdellidae) from Kii Peninsula, Japan (2018)
  • Author(s): Takafumi Nakano
  • Journal Title: Species Diversity Volume: 印刷中
  • Peer Reviewed
• [Journal Article] A new species of Brevitalitrus (Crustacea: Amphipoda: Talitridae) from Malaysia (2018)
  • Author(s): Ko Tomikawa, Takafumi Nakano, B. H. Ross Othman, Hiroshi Morino
  • Journal Title: Species Diversity Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A new species of Orobdella (Hirudinida: Arhynchobdellida: Orobdellidae) from Japan reveals the function of the Orobdella gastroporal duct (2017)
  • Author(s): Takafumi Nakano
  • Journal Title: Zoological Science Volume: 34 Issue: 2 Pages: 161-172
  • DOI: 10.2108/zs160167
  • Peer Reviewed
• [Journal Article] A new species of the genus Nicippe from Japan (Crustacea: Amphipoda: Pardaliscidae) (2017)
  • Author(s): Matsukami, S.; Nakano, T.; Tomikawa, K.
  • Journal Title: ZooKeys Volume: 668 Pages: 33-47
  • DOI: 10.3897/zookeys.668.12181
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of the Systematic Status of Geographical Variations in Arcuphantes hibanus (Arachnida: Araneae: Linyphiidae), with Descriptions of Two New Species (2017)
  • Author(s): Nakano Takafumi、Ihara Yoh、Kumasaki Yusuke、Baba Yuki G.、Tomikawa Ko
  • Journal Title: Zoological Science Volume: 34 Issue: 4 Pages: 331-344
  • DOI: 10.2108/zs160168
  • NAID: 40021289732
  • Peer Reviewed
• [Journal Article] A New Quadrannulate Species of <i>Orobdella</i> (Hirudinida: Arhynchobdellida: Orobdellidae) from Pingtung, Taiwan (2017)
  • Author(s): Takafumi Nakano, Yi-Te Lai
  • Journal Title: Species Diversity Volume: 22 Issue: 2 Pages: 143-150
  • DOI: 10.12782/specdiv.22.143
  • NAID: 130006246803
  • ISSN: 1342-1670, 2189-7301
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Low genetic diversity in Ozobranchus jantseanus (Hirudinida: Ozobranchidae) in Japan: possibility of introduction with their host turtles. (2017)
  • Author(s): Takafumi Nakano, Ryuta Nakamura, Susumu Ohtsuka, Toru Suzuki, Dai Suzuki
  • Journal Title: Parasitology International Volume: 受理済み Issue: 6 Pages: 1-19
  • DOI: 10.1016/j.parint.2017.08.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A new landhopper genus and species (Crustacea: Amphipoda: Talitridae) from Annamite Range, Vietnam (2017)
  • Author(s): Yuya Suzuki, Takafumi Nakano, Son Truong Nguyen, Anh Thi Thu Nguyen, Hiroshi Morino, Ko Tomikawa
  • Journal Title: Raffles Bulletin of Zoology Volume: 65 Pages: 304-315
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The enigmatic groundwater amphipod Awacaris kawasawai revisited: synonymisation of the genus Sternomoera, with molecular phylogenetic analyses of Awacaris and Sternomoera species (Crustacea : Amphipoda : Pontogeneiidae) (2017)
  • Author(s): Ko Tomikawa, Masaki Kyono, Keiko Kuribayashi, Takafumi Nakano
  • Journal Title: Invertebrate Systematics Volume: 31 Issue: 2 Pages: 125-140
  • DOI: 10.1071/is16037
  • Peer Reviewed
• [Journal Article] Deep-sea amphipod genus Eurythenes from Japan, with a description of a new Eurythenes species from off Hokkaido (Crustacea: Amphipoda: Lysianassoidea) (2017)
  • Author(s): Narahara-Nakano Yukiko、Nakano Takafumi、Tomikawa Ko
  • Journal Title: Marine Biodiversity Volume: 48 Issue: 1 Pages: 603-620
  • DOI: 10.1007/s12526-017-0758-4
  • Peer Reviewed / Open Access
• [Journal Article] Praobdellidae (Hirudinida: Arhynchobdellida) is not specific only to the mucous-membrane after all: discovery of a praobdellid leech feeding on the Japanese freshwater crab Geothelphusa dehaani (2017)
  • Author(s): Nakano, T.; Tomikawa, K.; Sakono, T.; Yoshikawa, N.
  • Journal Title: Parasitology International Volume: 66 Issue: 3 Pages: 210-213
  • DOI: 10.1016/j.parint.2017.01.018
  • Peer Reviewed
• [Journal Article] Two new species of Jesogammarus from Japan (Crustacea, Amphipoda, Anisogammaridae), with comments on the validity of the subgenera Jesogammarus and Annanogammarus (2017)
  • Author(s): Tomikawa, K.; Nakano, T.; Hanzawa, N.
  • Journal Title: Zoosystematics and Evolution Volume: 93 Issue: 2 Pages: 189-210
  • DOI: 10.3897/zse.93.12125
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A new species of Tritetrabdella (Hirudinida: Hirudiniformes: Haemadipsidae) (2016)
  • Author(s): Takafumi Nakano, Ekgachai Jeratthitikul, Tao Thien Nguyen, Somsak Panha
  • Journal Title: Raffles Bulletin of Zoology Volume: 64 Pages: 105-116
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] First Record of <i>Mimobdella japonica</i> (Hirudinida: Arhynchobdellida: Salifidae) from Hachijojima Island, Izu Islands, Japan, with a Comment on the Genetic Diversity of the Species (2016)
  • Author(s): Takafumi Nakano, Victor Benno Meyer-Rochow
  • Journal Title: Species Diversity Volume: 21 Issue: 1 Pages: 49-54
  • DOI: 10.12782/sd.21.1.049
  • NAID: 130006832082
  • ISSN: 1342-1670, 2189-7301
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] First Record of <i>Poecilobdella nanjingensis</i> (Hirudinida: Arhynchobdellida: Hirudinidae) from Taiwan and its Molecular Phylogenetic Position within the Family (2016)
  • Author(s): Takafumi Nakano, Yi-Te Lai
  • Journal Title: Species Diversity Volume: 21 Issue: 2 Pages: 127-134
  • DOI: 10.12782/sd.21.2.127
  • NAID: 130006832088
  • ISSN: 1342-1670, 2189-7301
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A new species of Priscomilitaris from the Seto Inland Sea, Japan (Crustacea: Amphipoda: Priscomilitaridae) (2016)
  • Author(s): Tomikawa, K.; Tanaka, H.; Nakano, T.
  • Journal Title: ZooKeys Volume: 607 Pages: 25-35
  • DOI: 10.3897/zookeys.607.9379
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A molecular phylogeny of Pseudocrangonyx from Japan, including a new subterranean species (Crustacea, Amphipoda, Pseudocrangonyctidae) (2016)
  • Author(s): Tomikawa, K.; Nakano, T.; Sato, A.; Onodera, Y.; Ohtaka, A.
  • Journal Title: Zoosystematics and Evolution Volume: 92 Issue: 2 Pages: 187-202
  • DOI: 10.3897/zse.92.10176
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A new quadrannulate species of Orobdella (Hirudinida, Arhynchobdellida, Orobdellidae) from western Honshu, Japan (2016)
  • Author(s): Takafumi Nakano
  • Journal Title: ZooKeys Volume: 553 Pages: 33-51
  • DOI: 10.3897/zookeys.553.6723
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Four new species of the genus Orobdella from Shikoku and Awajishima island, Japan (Hirudinida, Arhynchobdellida, Orobdellidae) (2016)
  • Author(s): Takafumi Nakano
  • Journal Title: Zoosystematics and Evolution Volume: 92 Issue: 1 Pages: 79-102
  • DOI: 10.3897/zse.91.7616
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A new predatory leech from Vietnam (Hirudinida : Arhynchobdellida : Salifidae): its phylogenetic position with comments on the classification of the family (2015)
  • Author(s): Takafumi Nakano, Son Truong Nguyen
  • Journal Title: Invertebrate Systematics Volume: 29 Issue: 5 Pages: 473-486
  • DOI: 10.1071/is15008
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 山地にうごめく巨食性ヒル類：分類学的再検討と「胃通管」 (2017)
  • Author(s): 中野隆文
  • Organizer: 第16回日本分類学会連合公開シンポジウム
  • Place of Presentation: 国立科学博物館，東京
  • Year and Date: 2017-01-07
  • Invited
• [Presentation] ベトナムより得られたハマトビムシ科端脚目の1未記載種 (2017)
  • Author(s): 鈴木悠矢・中野隆文・富川光・森野浩
  • Organizer: 日本動物分類学会第53回大会
• [Presentation] Revisiting Asian macrophagous leeches: veiled taxonomic status of giant worms (2016)
  • Author(s): Takafumi Nakano
  • Organizer: The 22nd International Conference of Zoology
  • Place of Presentation: Okinawa Convention Center, Okinawa
  • Year and Date: 2016-11-14
  • Int'l Joint Research / Invited
• [Presentation] アジアにおける捕食性ヒル類（イシビル形亜目）の多様性と系統 (2015)
  • Author(s): 中野隆文
  • Organizer: 環形動物の分類学に関するシンポジウム：日本およびその周辺におけるこれまでの知見と今後の課題
  • Place of Presentation: 東京大学大気海洋研究所，柏
  • Year and Date: 2015-11-19
  • Invited
• [Remarks] T. Nakano (Systematic Hirudinologist)
  • URL: http://zoo.zool.kyoto-u.ac.jp/~nakano/index.html
• [Remarks] 中野隆文（ヒル分類学）個人 Web Page
  • URL: http://hiru-bunrui.sakura.ne.jp/index.html
• [Remarks] 中野隆文（ヒル分類学）個人 Web Page
  • URL: http://hiru-bunrui.sakura.ne.jp/