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帯電液滴重畳反応性プラズマジェットによる高分子薬剤の高効率細胞内導入

Research Project

Project/Area Number 15J01427
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Plasma electronics
Research InstitutionTohoku University

Principal Investigator

佐々木 渉太  東北大学, 工学研究科, 特別研究員(PD)

Project Period (FY) 2015-04-24 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2017: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2016: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2015: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords大気圧プラズマ / HEPES / 水酸基ラジカル / スーパーオキシドアニオンラジカル / ペルオキシナイトライト / TRPチャネル / 薬剤導入 / 細胞膜輸送 / 過酸化水素 / ライブイメージング / カルシウムイオンチャネル / レーザー誘起蛍光法
Outline of Annual Research Achievements

本研究では,帯電液滴重畳反応性プラズマジェットによる高分子薬剤の高効率細胞内導入実現を目的として,大気圧プラズマ照射が誘発する薬剤(遺伝子,タンパク質,抗がん剤)の細胞膜輸送機構を明らかにする実験を行った結果,以下のことを明らかにした.
1.大気圧プラズマ照射HEPES緩衝生理食塩水(HBS)中に形成される化学反応系の理解: 前年度までに,プラズマ直接照射中にはOHラジカルが支配的に生成されることを明らかにしていた.今年度は,OHラジカルの主な反応相手として溶液に多量に含まれる有機物であるHEPESに着目して,化学反応系の理解を試みた.HEPES酸化物のESR測定及び各種選択的化学プローブによる検討の結果,プラズマ照射HBS中では,HEPESのラジカル化が持続的に起きており,数分に亘ってO2-/ONOO-といった短寿命活性種の低ドーズ放出が起きていることが示された.
2.プラズマ照射HBS中の持続的O2-/ONOO-生成が誘導する細胞膜輸送の測定: 前年度までに,プラズマ照射HBSがTRPチャネル介在性の薬剤模擬分子摂取を誘導することを明らかにしていたが,上記のO2-/ONOO-との関連を調べた. O2-の不均化酵素であるスーパーオキシドディスムターゼを用いて,O2-が関わる反応系の関与を見出した.さらに,既存のONOO-ドナーであるSIN-1の添加によって,プラズマと同等の効果を得たことから,主要な作用因子はO2-/ONOO-である可能性が極めて高いと結論付けた.
以上より,本研究では,第一にHBSに大気圧プラズマを照射することで,従来法に比べ極めて低いコスト(1/10000以下)でO2-/ONOO-を数分間に亘って供給する特異な反応系を形成できることを発見した.第二に,この反応系を細胞に作用させることで,TRPチャネル介在性薬剤摂取を促進可能であることを実証した.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (74 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Peer Reviewed: 9 results,  Open Access: 6 results,  Acknowledgement Compliant: 5 results) Presentation (53 results) (of which Int'l Joint Research: 27 results,  Invited: 21 results) Book (2 results) Remarks (5 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] University of Minnesota(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Direct plasma stimuli including electrostimulation and OH radical induce transient increase in intracellular Ca2+ and uptake of a middle-size membrane-impermeable molecule2018

    • Author(s)
      S. Sasaki,Y. Hokari,A. Kumada,M. Kanzaki,and T. Kaneko
    • Journal Title

      Plasma Processes and Polymers

      Volume: 15 Issue: 1

    • DOI

      10.1002/ppap.201700077

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of Cell Function Using Gas-Liquid Interfacial Plasmas2018

    • Author(s)
      KANEKO Toshiro、SASAKI Shota、HONDA Ryosuke、SATO Takehiko、KANZAKI Makoto
    • Journal Title

      Vacuum and Surface Science

      Volume: 61 Issue: 3 Pages: 143-149

    • DOI

      10.1380/vss.61.143

    • NAID

      130006476162

    • ISSN
      2433-5835, 2433-5843
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cold atmospheric plasma enhances osteoblast differentiation2017

    • Author(s)
      Tominami K, Kanetaka H, Sasaki S, Mokudai T, Kaneko T, Niwano Y
    • Journal Title

      PLoS One

      Volume: 12 Issue: 7 Pages: e0180507-e0180507

    • DOI

      10.1371/journal.pone.0180507

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 気液界面大気圧プラズマによる殺菌法と医療応用2017

    • Author(s)
      金子 俊郎、佐々木 渉太、高島 圭介
    • Journal Title

      工業材料

      Volume: 65 Pages: 40-44

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Gas-liquid interfacial plasmas producing reactive species for cell membrane permeabilization2017

    • Author(s)
      T. Kaneko, S. Sasaki, K. Takashima, and M. Kanzaki
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 60 Issue: 1 Pages: 3-11

    • DOI

      10.3164/jcbn.16-73

    • NAID

      130005188908

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Calcium influx through TRP channels induced by short-lived reactive species in plasma-irradiated solution2016

    • Author(s)
      S. Sasaki, M. Kanzaki, and T. Kaneko
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 25728-25728

    • DOI

      10.1038/srep25728

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Roles of charged particles and reactive species on cell membrane permeabilization induced by atmospheric-pressure plasma irradiation2016

    • Author(s)
      S. Sasaki, M. Kanzaki, Y. Hokari, K. Tominami, T. Mokudai, H. Kanetaka, and T. Kaneko
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 55 Issue: 7S2 Pages: 07LG04-07LG04

    • DOI

      10.7567/jjap.55.07lg04

    • NAID

      210000146888

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of plasma-induced cell membrane permeabilization: focus on OH radical distribution2016

    • Author(s)
      S. Sasaki, R. Honda, Y. Hokari, K. Takashima, M. Kanzaki, and T. Kaneko
    • Journal Title

      Journal of Physics D: Applied Physics

      Volume: 49 Issue: 33 Pages: 334002-334002

    • DOI

      10.1088/0022-3727/49/33/334002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] プラズマ刺激による細胞応答と応用 その1.2016

    • Author(s)
      佐藤岳彦, 横山茉代, 城倉浩平, 金子俊郎, 佐々木渉太, 神崎展, 太田貴之
    • Journal Title

      機械の研究

      Volume: 68 Pages: 147-157

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Improvement of Cell Membrane Permeability Using a Cell-Solution Electrode for Generating Atmospheric-Pressure Plasma2015

    • Author(s)
      T. Kaneko, S. Sasaki, Y. Hokari, S. Horiuchi, R. Honda, and M. Kanzaki
    • Journal Title

      Biointerphases

      Volume: 10 Issue: 2 Pages: 029521-029521

    • DOI

      10.1116/1.4921278

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Apoptotic Effects on Cultured Cells of Atmospheric-Pressure Plasma Produced Using Various Gases2015

    • Author(s)
      K. Tominami, H. Kanetaka, T. Kudo, S. Sasaki, and T. Kaneko
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 55 Issue: 1S Pages: 01AF03-01AF03

    • DOI

      10.7567/jjap.55.01af03

    • NAID

      210000145990

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatially-Selective Membrane Permeabilization Induced by Cell-Solution Electrode Atmospheric Pressure Plasma Irradiation2015

    • Author(s)
      S. Sasaki,Y. Hoakari, M. Kanzaki and T. Kaneko
    • Journal Title

      Proceedings of 9th International Conference on Reactive Plasmas (ICRP) / 68th Annual Gaseous Electronics Conference (GEC) / 33rd Symposium on Plasma Processing (SPP)

      Volume: -

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Presentation] プラズマ分子導入の高効率化に向けた短寿命活性種の測定2018

    • Author(s)
      鄭 悦星,佐々木 渉太,神崎 展,金子 俊郎
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 薬剤分子導入へ向けた液相中プラズマの電極構造の最適化2018

    • Author(s)
      本田 竜介,佐々木 渉太,髙島 圭介,神崎 展,佐藤 岳彦,金子 俊郎
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Drug introduction by prolonged reactive species production using in-liquid plasmas2017

    • Author(s)
      R. Honda, Y. Hokari, S. Sasaki, M. Kanzaki, T. Sato, and T. Kaneko
    • Organizer
      The 4th Japan-Taiwan Workshop on Plasma Life Science and Technology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プラズマ生成短寿命活性種によるTRPチャネル活性化と細胞膜輸送促進2017

    • Author(s)
      金子 俊郎,佐々木 渉太,立川 正憲,神崎 展
    • Organizer
      第13回TRPチャネル研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Gas-liquid interfacial plasmas for enhancing gene transfer into living cells2017

    • Author(s)
      T. Kaneko, S. Sasaki, K. Takashima, M. Kanzaki, H. Kanetaka, M. Tachikawa
    • Organizer
      The 44th European Physical Society Conference on Plasma Physics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Gas-liquid interfacial plasmas for novel gene transfer systems2017

    • Author(s)
      T. Kaneko, S. Sasaki, K. Takashima, T. Sato, M. Kanzaki
    • Organizer
      The International Conference on Phenomena in Ionized Gases (ICPIG) 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Investigation of compositions in plasma-irradiated buffer evoking TRP-channel mediated calcium response2017

    • Author(s)
      S. Sasaki, Y. Zheng, M. Kanzaki, and T. Kaneko
    • Organizer
      The International Conference on Phenomena in Ionized Gases (ICPIG) 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プラズマ由来複合刺激を用いた細胞膜輸送の能動的制御2017

    • Author(s)
      佐々木 渉太,鄭 悦星,本田 竜介,高島 圭介,神崎 展,金子 俊郎
    • Organizer
      第78回応用物理学会秋季学術講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 薬剤導入に寄与するプラズマ照射溶液中活性種の探究2017

    • Author(s)
      鄭 悦星,佐々木 渉太,神崎 展,金子 俊郎
    • Organizer
      第78回応用物理学会秋季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Gas-Liquid Interfacial Plasmas Enhancing Gene Transfer by Controlling Behavior of Reactive Species2017

    • Author(s)
      T. Kaneko, S. Sasaki, K. Takashima, and M. Kanzaki
    • Organizer
      1st Asia-Pacific Conference on Plasma Physics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 液相中プラズマ由来短寿命活性種の薬剤導入への効果2017

    • Author(s)
      本田 竜介,保苅 雄太郎,佐々木 渉太,神崎 展,佐藤 岳彦,金子 俊郎
    • Organizer
      Plasma Conference 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 気液界面プラズマ照射による細胞膜輸送促進の機序解明2017

    • Author(s)
      金子 俊郎,佐々木 渉太,立川 正憲,神崎 展
    • Organizer
      Plasma Conference 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Multiple Stimuli of Gas-Liquid Interfacial Plasmas Enhancing Drug Transfer into Cells2017

    • Author(s)
      T. Kaneko, S. Sasaki, K. Takashima, and M. Kanzaki
    • Organizer
      The 10th EU-Japan Joint Symposium on Plasma Processing
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Gas-liquid interfacial plasmas for enhancing drug introduction into living cells2017

    • Author(s)
      T. Kaneko, S. Sasaki, K. Takashima, and M. Kanzaki
    • Organizer
      The 26th International Toki Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Gas-Liquid Interfacial Plasmas for Structure-Controlled Nano-Material Synthesis and Cellular-Function Control2017

    • Author(s)
      T. Kaneko, S. Sasaki, M. Kawashita, and M. Kanzaki
    • Organizer
      10th Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] プラズマによる遺伝子導入を若手が語る2017

    • Author(s)
      佐々木 渉太,本村 英樹,池田 善久
    • Organizer
      新学術領域研究「プラズマ医療科学の創成」,プラズマ医療科学の未来を若手が語る会 in 日本橋
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Membrane Permeabilizing Effects of Saline Treated with Different Types of Gas-Liquid Interfacial Plasmas2017

    • Author(s)
      T. Kaneko, S. Sasaki, Y. Hokari, Y. Zheng, K. Takashima, and M. Kanzaki
    • Organizer
      34th Symposium on Plasma Processing (SPP34) / The 29th Symposium on Plasma Science for Materials (SPSM29)
    • Place of Presentation
      北海道大学 (北海道,札幌市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Activation Mechanism of Cell Membrane Transport by Short-lived Reactive Species in Plasma-irradiated Solution2017

    • Author(s)
      S. Sasaki, Y. Zheng, M. Kanzaki, and T. Kaneko
    • Organizer
      The 1st International Conference on Plasma Medical Science Innovation
    • Place of Presentation
      Nagoya University (Nagoya, Japan)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PLASMA GENE TRANSFECTION: ENHANCEMENT OF CELL MEMBRANE PERMEABILITY WITHOUT DAMAGE USING GAS-LIQUID INTERFACIAL MICRO-SCALE PLASMAS2017

    • Author(s)
      T. Kaneko, M. Jinno, T. Sato, S. Sasaki, M. Kanzaki, M. Tachikawa, H. Kanetaka
    • Organizer
      The 1st International Conference on Plasma Medical Science Innovation
    • Place of Presentation
      Nagoya University (Nagoya, Japan)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 異種気液界面プラズマによる細胞膜透過性促進の比較2017

    • Author(s)
      金子俊郎,佐々木渉太,保苅雄太郎,鄭悦星,高島圭介,神崎展
    • Organizer
      第64回応用物理学会春季学術講演会
    • Place of Presentation
      パシフィコ横浜(神奈川県,横浜市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 大気圧低温プラズマによるTRPチャネル活性化と遺伝子導入2017

    • Author(s)
      金子俊郎,佐々木渉太,鄭悦星,神崎展
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台国際センター(宮城県,仙台市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] プラズマ由来液中活性種が誘導する細胞内カルシウム濃度上昇・振動2016

    • Author(s)
      佐々木渉太,保苅雄太郎,神崎展,金子俊郎
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学(東京都,目黒区)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 液相微小プラズマを用いた接着細胞への薬剤分子導入2016

    • Author(s)
      保苅雄太郎, 佐々木渉太, 神崎展, 佐藤岳彦, 金子俊郎
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学(東京都,目黒区)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of Plasma-produced Reactive Species on Cell Membrane Permeabilization2016

    • Author(s)
      S. Sasaki,Y. Hoakari, M. Kanzaki and T. Kaneko
    • Organizer
      International Workshop on Plasma Medical Science Innovation
    • Place of Presentation
      Nagoya University (Nagoya, Japan)
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 大気圧プラズマ照射によるヒト大腸がん細胞の細胞膜輸送変動の解明2016

    • Author(s)
      立川正憲, 佐野大知, 佐々木渉太, 神崎展, 寺崎哲也, 金子俊郎
    • Organizer
      日本薬剤学会 第31年会
    • Place of Presentation
      長良川国際会議場(岐阜県,岐阜市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] ATOMOSPHERIC-PRESSURE PLASMA-INDUCED CELLULAR RESPONSES IN HUMAN COLORECTAL ADENOCARCINOMA CACO-2 CELLS: A STUDY OF SWATHTM-BASED COMPREHENSIVE QUANTITATIVE PROTEOMICS2016

    • Author(s)
      M. Tachikawa, D. Sano, S. Sasaki, M. Kanzaki, T. Terasaki, and T. Kaneko
    • Organizer
      43rd IEEE International Conference on Plasma Science
    • Place of Presentation
      The Banff Centre (Alberta, Canada)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PLASMA-IRRADIATED SOLUTION AS DRUG PERMEATION ENHANCER2016

    • Author(s)
      T. Kaneko, K. Kikuchi, S. Sasaki, and M. Kanzaki
    • Organizer
      43rd IEEE International Conference on Plasma Science
    • Place of Presentation
      The Banff Centre (Alberta, Canada)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activation of Sensory Channels in Cell Membrane Using Plasma-Generated Reactive Species2016

    • Author(s)
      S. Sasaki, M. Kanzaki and T. Kaneko
    • Organizer
      Gordon Research Seminar on Plasma Processing Science
    • Place of Presentation
      Proctor Academy (Andover, New Hampshire, USA)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Spatial Mapping of Gas and Aqueous Phase OH Radicals and Plasma-Induced Effect on Cell Membrane Permeabilization2016

    • Author(s)
      T. Kaneko, S. Sasaki, R. Honda, Y. Hokari, K. Takashima, and M. Kanzaki
    • Organizer
      6th International Conference on Plasma Medicine (ICPM6)
    • Place of Presentation
      Slovak University of Technology (Bratislava, Slovakia)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interaction between Plasma-activated Calcium Ion Channel and Uptake of Drug-simulated Molecule2016

    • Author(s)
      S. Sasaki, K. Kikuchi, M. Kanzaki and T. Kaneko
    • Organizer
      6th International Conference on Plasma Medicine (ICPM6)
    • Place of Presentation
      Slovak University of Technology (Bratislava, Slovakia)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of Multi-Stimuli on Cell Membrane Permeability in Micro Solution Plasma2016

    • Author(s)
      Y. Hokari, S. Sasaki, M. Kanzaki, T. Sato, and T. Kaneko
    • Organizer
      6th International Conference on Plasma Medicine (ICPM6)
    • Place of Presentation
      Slovak University of Technology (Bratislava, Slovakia)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞膜輸送に対する液相中プラズマの物理的・化学的刺激の効果2016

    • Author(s)
      保苅雄太郎, 佐々木渉太, 神崎展, 佐藤岳彦, 金子俊郎
    • Organizer
      第77回応用物理学会秋季学術講演会
    • Place of Presentation
      朱鷺メッセ(新潟県,新潟市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞膜輸送制御に向けたプラズマ誘起活性種・電位分布計測2016

    • Author(s)
      佐々木渉太,保苅雄太郎,高島圭介,熊田亜紀子,神崎展,金子俊郎
    • Organizer
      第77回応用物理学会秋季学術講演会
    • Place of Presentation
      朱鷺メッセ(新潟県,新潟市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] プラズマ照射溶液中短寿命活性種に対するTRPチャネル介在細胞内カルシウム応答2016

    • Author(s)
      佐々木渉太,神崎展,金子俊郎
    • Organizer
      第77回応用物理学会秋季学術講演会
    • Place of Presentation
      朱鷺メッセ(新潟県,新潟市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 薬剤導入に寄与する液相中プラズマ由来因子の同定2016

    • Author(s)
      保苅雄太郎, 佐々木渉太, 神崎展, 佐藤岳彦, 金子俊郎
    • Organizer
      プラズマ・核融合学会第33回年会
    • Place of Presentation
      東北大学 (宮城県,仙台市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 接着細胞への薬剤分子導入へ向けた液中微小プラズマの最適化2016

    • Author(s)
      鄭悦星,佐々木渉太,神崎展,金子俊郎
    • Organizer
      プラズマ・核融合学会第33回年会
    • Place of Presentation
      東北大学 (宮城県,仙台市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Gas-Liquid Interfacial Atmospheric Pressure Plasmas for Medical Applications2016

    • Author(s)
      T. Kaneko, S. Sasaki, K. Takashima, T. Sato, H. Kanetaka, M. Tachikawa, and M. Kanzaki
    • Organizer
      The 3rd Japan-Taiwan Workshop on Plasma Life Science and Technology
    • Place of Presentation
      Ming Chi University of Technology (New Taipei City, Taiwan)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Onset Time of Cell-membrane Permeabilization by Plasma-Produced Reactive Species2016

    • Author(s)
      Y. Zheng, S. Sasaki, M. Kanzaki and T. Kaneko
    • Organizer
      第26回日本MRS年次大会
    • Place of Presentation
      横浜市開港記念会館 (神奈川県,横浜市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Plasma Gene Transfection: Effects of Plasma Stimuli on Cell Membrane Permeabilization2016

    • Author(s)
      T. Kaneko, S. Sasaki, K. Takashima, T. Sato, H. Kanetaka, M. Tachikawa, and M. Kanzaki
    • Organizer
      第26回日本MRS年次大会
    • Place of Presentation
      横浜市開港記念会館 (神奈川県,横浜市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Macromolecule Transfer through Cell Membrane Using Micro-Scale Plasma Generated in High-Conductivity Liquid2015

    • Author(s)
      Y. Hokari, S. Sasaki, M. Kanzaki, T. Sato, and T. Kaneko
    • Organizer
      the 9th Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology (APSPT-9) and the 28th Symposium on Plasma Science for Materials (SPSM-28)
    • Place of Presentation
      Nagasaki University (Nagasaki, Japan)
    • Year and Date
      2015-12-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Generation of micro solution plasma for minimally-invasive in vivo gene transfection2015

    • Author(s)
      T. Kaneko, Y. Hokari, S. Sasaki, M. Kanzaki, and T. Sato
    • Organizer
      2015 Materials Research Society(MRS) Fall Meeting & Exhibit
    • Place of Presentation
      Hynes Convention Center (Boston, USA)
    • Year and Date
      2015-11-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 接着細胞への薬剤分子導入へ向けた液中微小プラズマの最適化2015

    • Author(s)
      保苅雄太郎,佐々木渉太,神崎展,佐藤岳彦,金子俊郎
    • Organizer
      プラズマ・核融合学会 第32回年会
    • Place of Presentation
      名古屋大学(愛知県,名古屋市)
    • Year and Date
      2015-11-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Plasma Stimuli for Enhancement of Cell Membrane Permeability2015

    • Author(s)
      T. Kaneko, S. Sasaki, Y. Hokari, and M. Kanzaki
    • Organizer
      The Twelfth International Conference on Flow Dynamics (ICFD2015)
    • Place of Presentation
      Sendai International Center (Sendai, Japan)
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Spatially-Selective Membrane Permeabilization Induced by Cell-Solution Electrode Atmospheric Pressure Plasma Irradiation2015

    • Author(s)
      S. Sasaki,Y. Hoakari, M. Kanzaki, and T. Kaneko
    • Organizer
      9th International Conference on Reactive Plasmas / 68th Annual Gaseous Electronics Conference / 33rd Symposium on Plasma Processing
    • Place of Presentation
      Hawaii convention Center (Honolulu, Hawaii, USA)
    • Year and Date
      2015-10-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Localized Gene Transfection through Cell Membrane Stimulated by Micro Solution Plasma2015

    • Author(s)
      T. Kaneko, Y. Hokari, S. Sasaki, M. Kanzaki, and T. Sato
    • Organizer
      9th International Conference on Reactive Plasmas / 68th Annual Gaseous Electronics Conference / 33rd Symposium on Plasma Processing
    • Place of Presentation
      Hawaii convention Center (Honolulu, Hawaii, USA)
    • Year and Date
      2015-10-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cell Membrane Transport Using Atmospheric Pressure Plasmas in Contact with Liquid2015

    • Author(s)
      T. Kaneko, S. Sasaki, Y. Hokari, M. Kanzaki, and T. Sato
    • Organizer
      The 21st Korea-Japan Workshop on Advanced Plasma Processes and Diagnostics
    • Place of Presentation
      Osaek Greenyard Hotel (Yangyang, Korea)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 非平衡大気圧プラズマ刺激による新作用機序遺伝子導入2015

    • Author(s)
      金子俊郎,佐々木渉太,保苅雄太郎,神崎展
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議場(愛知県,名古屋市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 大気圧プラズマ照射液中化学活性種に対する細胞感受性2015

    • Author(s)
      佐々木渉太,保苅雄太郎,神崎展,金子俊郎
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議場(愛知県,名古屋市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Localized Macromolecule Transfer through Cell Membrane by Micro Plasma in High-Conductivity Liquid2015

    • Author(s)
      保苅雄太郎,佐々木渉太,神崎展,佐藤岳彦,金子俊郎
    • Organizer
      平成27年度電気関連学会東北支部連合大会
    • Place of Presentation
      岩手県立大学 (岩手県,滝沢市)
    • Year and Date
      2015-08-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] プラズマ照射による細胞膜輸送制御と低浸襲遺伝子導入への応用2015

    • Author(s)
      金子俊郎,佐々木渉太,保苅雄太郎,神崎展
    • Organizer
      日本動物細胞工学会2015
    • Place of Presentation
      東北大学 (宮城県,仙台市)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Synergy Effects of Physical and Chemical Stimulations on Cell Membrane Transport Using Non-Equilibrium Atmospheric Pressure Plasma2015

    • Author(s)
      T. Kaneko, S. Sasaki, Y. Hokari, and M. Kanzaki
    • Organizer
      The 2nd Japan-Taiwan Workshop on Plasma Life Science and Technology
    • Place of Presentation
      Tohoku University (Sendai, Japan)
    • Year and Date
      2015-06-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Macromolecule Transfer Through Cell Membrane by Discharge in Liquid Using Nano-scale Electrode2015

    • Author(s)
      Y. Hokari, S. Sasaki, T. Aoyama, M. Kanzaki, T. Sato, and T. Kaneko
    • Organizer
      The 2nd Japan-Taiwan Workshop on Plasma Life Science and Technology
    • Place of Presentation
      Tohoku University (Sendai, Japan)
    • Year and Date
      2015-06-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spatially Localized Transfection Using Controlled Plasma Irradiation to Solution Containing Living Cells2015

    • Author(s)
      T. Kaneko, S. Sasaki, Y. Hokari, and M. Kanzaki
    • Organizer
      The 3rd International Workshop on Solution Plasma and Molecular Technologies (SPM-3) and 2nd International Mini-Workshop on Solution Plasma and Molecular Technologies (Mini SPM-2)
    • Place of Presentation
      Chulalongkorn University (Bangkok, Thailand)
    • Year and Date
      2015-05-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] プラズマ産業応用技術 表面処理から環境,医療,バイオ,農業 用途まで 第4章 医療・バイオ・農業への応用 細胞膜輸送に対するプラズマ刺激の効果2017

    • Author(s)
      金子 俊郎、佐々木 渉太、神崎 展
    • Total Pages
      321
    • Publisher
      シーエムシー出版
    • ISBN
      9784781312569
    • Related Report
      2017 Annual Research Report
  • [Book] 高度物理刺激と生体応答 プラズマ刺激による細胞膜輸送制御2017

    • Author(s)
      金子 俊郎、佐々木 渉太、神崎 展
    • Total Pages
      197
    • Publisher
      養賢堂
    • Related Report
      2017 Annual Research Report
  • [Remarks] 金子・加藤研究室ホームページ

    • URL

      http://www.plasma.ecei.tohoku.ac.jp/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] Researchmap

    • URL

      http://researchmap.jp/Shota_Sasaki/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] Research Gate

    • URL

      http://www.researchgate.net/profile/Shota_Sasaki2

    • Related Report
      2017 Annual Research Report
  • [Remarks] Researchgate

    • URL

      http://www.researchgate.net/profile/Shota_Sasaki2

    • Related Report
      2016 Annual Research Report
  • [Remarks] 金子・加藤研究室 ホームページ

    • URL

      http://www.plasma.ecei.tohoku.ac.jp/

    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 遺伝子または薬剤導入方法、および、遺伝子または薬剤導入剤2017

    • Inventor(s)
      金子 俊郎、佐々木 渉太
    • Industrial Property Rights Holder
      金子 俊郎、佐々木 渉太
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-170350
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-11-26   Modified: 2024-03-26  

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