• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

ベクターコントロールによる有機塩素系農薬汚染が家畜・ヒトに及ぼす毒性影響の解明

Research Project

Project/Area Number 15J02013
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Environmental agriculture(including landscape science)
Research InstitutionHokkaido University

Principal Investigator

Thompson Lesa (2017)  北海道大学, 獣医学研究科, 特別研究員(DC1)

THOMPSON LESA (2015-2016)  北海道大学, 獣医学研究科, 特別研究員(DC1)

Project Period (FY) 2015-04-24 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2017: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2016: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2015: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsDDT / Chicken / Transcriptomics / Metabolomics / MCF-7 cell culture / Free-range chicken / Human health risk / Microarray / KwaZulu-Natal / Free-ranging chickens / Indoor residual spraying / South Africa
Outline of Annual Research Achievements

Two manuscripts have been published. Various alterations in mRNA expression were detected in chickens exposed to DDTs in the field, including down-regulation of several avian beta-defensins. A manuscript on these findings is currently at the revision stage of publication.mRNA expression was assessed using qRT-PCR after in vitro exposure of p,p’-DDT in MCF-7 human breast cancer cells. Several genes relating to oxidative stress markers, xenobiotic metabolizing enzymes and transporters were significantly up-regulated after exposure. A manuscript has been submitted for peer-review.Samples from in vivo exposure of chickens to p,p’-DDT underwent further analysis. Liver histopathology did not show significant changes. qRT-PCR of liver samples confirmed altered gene expression in avian beta defensin (AvBD) genes, AvBD1 and AvBD7. Further, metabolomics analysis using hydrophilic interaction liquid chromatography (HILIC) revealed many significantly elevated metabolites relating to amino acid metabolism in exposed groups. These results are currently being prepared for journal submission.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (31 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (9 results) Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 10 results,  Invited: 1 results) Remarks (3 results) Funded Workshop (3 results)

  • [Int'l Joint Research] North West University/BioAssets Consultants/University of Johannesburg(South Africa)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Zagazig University(Egypt)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Gondar(Ethiopia)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Edinburgh/University of Glasgow/Chester Zoo(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Johannesburg/North West University/BioAssets Consultants(South Africa)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Zagazig University(Egypt)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Gondar(Ethiopia)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Johannesburg/North West University/BioAssets Consultants(South Africa)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Zagazig University(Egypt)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Human Health Risk from Consumption of Marine Fish Contaminated with DDT and Its Metabolites in Maputo Bay, Mozambique2018

    • Author(s)
      Thompson L. A.、Ikenaka Y.、Yohannes Y. B.、Ichise T.、Ito G.、Bortey-Sam N.、van Vuren J. J.、Wepener V.、Smit N. J.、Darwish W. S.、Nakayama S. M. M.、Mizukawa H.、Ishizuka M.
    • Journal Title

      Bulletin of Environmental Contamination and Toxicology

      Volume: 100 Issue: 5 Pages: 672-676

    • DOI

      10.1007/s00128-018-2323-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Concentrations and human health risk assessment of DDT and its metabolites in free-range and commercial chicken products from KwaZulu-Natal, South Africa2017

    • Author(s)
      Thompson L. A.、Ikenaka Y.、Yohannes Y. B.、van Vuren J. J.、Wepener V.、Smit N. J.、Darwish W. S.、Nakayama S. M. M.、Mizukawa H.、Ishizuka M.
    • Journal Title

      Food Additives and Contaminants: Part A

      Volume: 34 Issue: 11 Pages: 1959-1969

    • DOI

      10.1080/19440049.2017.1357209

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] β-carotene and retinol reduce benzo[a]pyrene-induced mutagenicity and oxidative stress via transcriptional modulation of xenobiotic metabolizing enzymes in human HepG2 cell line2017

    • Author(s)
      Darwish Wageh Sobhy、Ikenaka Yoshinori、Nakayama Shouta、Mizukawa Hazuki、Thompson Lesa A、Ishizuka Mayumi
    • Journal Title

      Environmental Science and Pollution Research

      Volume: 25 Issue: 7 Pages: 6320-6328

    • DOI

      10.1007/s11356-017-0977-z

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Organochlorine pesticide contamination of foods in Africa: incidence and public health significance2017

    • Author(s)
      L. A. Thompson, W. S. Darwish, Y. Ikenaka, S. M. Nakayama, H. Mizukawa and M. Ishizuka
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 4 Pages: 751-764

    • DOI

      10.1292/jvms.16-0214

    • NAID

      130005568160

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Vector-control pesticide DDT detected in free-ranging chickens from South Africa2015

    • Author(s)
      Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
    • Journal Title

      Proceedings: 7th International Toxicology Symposium in Africa

      Volume: 7 Pages: 4-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] First, do no harm: balancing the benefits of malaria control against the toxic effects of DDT use2017

    • Author(s)
      Thompson, L.A., Ikenaka, Y., Yohannes, Y., Assefa, A., van Vuren, J., Wepener, V., Smit, N., Vlok, W., Darwish, W.S., Nakayama, S., Mizukawa, H., Ishizuka, M.
    • Organizer
      5th Sapporo Summer Seminar for One Health
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparison of food products contaminated by DDTs in South Africa and Mozambique2017

    • Author(s)
      Thompson, L.A., Ikenaka, Y., Wepener, V., Ishizuka, M.
    • Organizer
      19th International Conference on One Health
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of DDTs on gallinacins in the avian immune system.2017

    • Author(s)
      Thompson, L.A., Ikenaka, Y., Yohannes, Y., van Vuren, J., Wepener, V., Smit, N., Assefa, A., Darwish, W.S., Nakayama, S., Mizukawa, H., Ishizuka, M.
    • Organizer
      The 10th International Meeting of the Asian Society of Conservation Medicine One Health in Asia Pacific
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic effects of the pesticide DDT on birds: lessons learned from a field study in South Africa.2016

    • Author(s)
      Thompson, L.A., Ikenaka, Y., Yohannes, Y., Tsubota, T., van Vuren, J., Wepener, V., Smit, N., Nakayama, S., Mizukawa, H., Ishizuka, M.
    • Organizer
      The 9th International Meeting of the Asian Society of Conservation Medicine One Health in Asia Pacific
    • Place of Presentation
      Taipei Zoo, Taiwan
    • Year and Date
      2016-10-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Playing “chicken” - a xenobiotic dilemma.2016

    • Author(s)
      Thompson, L.A., Ikenaka, Y., Yohannes, Y., Assefa, A., van Vuren, J., Wepener, V., Smit, N., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
    • Organizer
      4th Sapporo Summer Seminar for One Health
    • Place of Presentation
      Hokkaido University, Sapporo, Hokkaido, Japan
    • Year and Date
      2016-09-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contamination of eggs from free-ranging chickens in a malaria-endemic region using DDT as vector control.2016

    • Author(s)
      Thompson, L.A., Ikenaka, Y., Yohannes, Y.B., van Vuren, J.J., Wepener, V., Smit, N.J., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
    • Organizer
      ISEE-ISES Asia Chapter
    • Place of Presentation
      Hokkaido University, Sapporo, Hokkaido, Japan
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Risks versus benefits of controlling vector-borne diseases using insecticides: a case example in South Africa.2016

    • Author(s)
      Thompson, L.A., Ikenaka, Y., Yohannes, Y.B., van Vuren, J.J., Wepener, V., Smit, N.J., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
    • Organizer
      2nd International Conference on One Medicine One Science
    • Place of Presentation
      The Commons Hotel, Minneapolis, USA
    • Year and Date
      2016-04-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Vector-contol pesticide DDT detected in free-ranging chickens from South Africa2015

    • Author(s)
      Lesa A THOMPSON, Yoshinori IKENAKA, Yared B YOHANNES, Johan J VAN VUREN, Victor WEPENER, Nico J SMIT, Wynand VLOK, Shouta M M NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
    • Organizer
      3rd Sapporo Summer Seminar for One Health
    • Place of Presentation
      Hokkaido University, Sapporo, Hokkaido, Japan
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Vector-control pesticide DDT detected in free-ranging chickens from South Africa2015

    • Author(s)
      Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
    • Organizer
      7th International Toxicology Symposium in Africa
    • Place of Presentation
      Garden Court Hotel OR Tambo, Johannesburg, South Africa
    • Year and Date
      2015-08-31
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Vector-control pesticides disseminating via international waterways to accumulate in free-ranging freshwater and marine fish species2015

    • Author(s)
      Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
    • Organizer
      Vth International Wildlife Management Congress
    • Place of Presentation
      Sapporo Convention Center, Sapporo, Hokkaido, Japan
    • Year and Date
      2015-07-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Vector-control pesticide DDT detected in free-ranging chickens from South Africa2015

    • Author(s)
      Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
    • Organizer
      24th Symposium on Environmental Chemistry
    • Place of Presentation
      Sapporo Convention Center, Sapporo, Hokkaido, Japan
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Remarks] Vetmed Tox Hokkaido University

    • URL

      http://tox.vetmed.hokudai.ac.jp/en/

    • Related Report
      2017 Annual Research Report 2015 Annual Research Report
  • [Remarks] Vetmed Tox Hokkaido University

    • Related Report
      2016 Annual Research Report
  • [Remarks] http://tox.vetmed.hokudai.ac.jp/en/

    • Related Report
      2016 Annual Research Report
  • [Funded Workshop] 4th Sapporo Summer Seminar for One Health, committee chairperson for student session ”Collaboration in One Health: what’s your role?”2016

    • Place of Presentation
      Hokkaido University, Sapporo, Hokkaido, Japan
    • Year and Date
      2016-09-20
    • Related Report
      2016 Annual Research Report
  • [Funded Workshop] South Africa - Japan bipartite research symposium, invited presenter, “Extraneous DDT residues in free-ranging chicken products from KwaZulu-Natal, South Africa”2016

    • Place of Presentation
      Hokkaido University, Sapporo, Hokkaido, Japan
    • Related Report
      2016 Annual Research Report
  • [Funded Workshop] IWMC, crayfish eradication program2015

    • Place of Presentation
      Toyako-cho
    • Year and Date
      2015-07-31
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-11-26   Modified: 2024-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi