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リバースジェネティクス法を用いた新遺伝子型RSウイルス(ON1)の増殖能の解析

Research Project

Project/Area Number 15J05618
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Virology
Research InstitutionTohoku University

Principal Investigator

MALASAO RUNGNAPA  東北大学, 医学系研究科, 特別研究員(DC2)

Project Period (FY) 2015-04-24 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2016: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2015: ¥900,000 (Direct Cost: ¥900,000)
KeywordsRespiratory syncytial / Reverse genetics / NA1 / ON1
Outline of Annual Research Achievements

I analyzed molecular characterization of HRSV in Biliran Island, Philippines over three seasons in 2013, 2014, and 2015. Nasopharyngeal swabs were collected from hospitalized children with severe pneumonia at the Biliran Provincial Hospital. Samples were screened by using real-time polymerase chain reaction (PCR). Genotypes were determined using conventional PCR and sequencing of the 2nd hypervariable region (HVR) of the G gene. Of 1,240 sample tested, 356 (28.7%) were positive for HRSV including 183 (51.4%) HRSV-A and 173 (48.6%) HRSV-B. HRSV-A were further divided into two genotypes; NA1 and ON1. Both NA1 and ON1 were co-circulating in 2013, only ON1 remained to be circulating in 2014. A novel ON1 variant, which was phylogenetically distinct from other ON1 strains, was detected in 2014. These novel ON1 viruses possess three unique amino acid substitutions (K212R, K213G, and K223G) in the 2nd HVR of the G gene, which had not been found in other countries. All HRSV-B viruses were identified as genotype BA9 except two samples identified as GB2. Both HRSV-A and -B were co-circulating in 2013 and 2014 seasons, but most of HRSV detected in 2015 season were HRSV-B. Most of amino acid sites of the 2nd HVR of the G gene were under purifying/negative selection. This study can build on the molecular characterization to contribute the advance of molecular genetics on HRSV in the Philippines. Moreover, I made the manuscript of complete genome sequences of HRSV-A in the Philippines that I did last year.

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (10 results)

All 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (2 results) Remarks (2 results)

  • [Int'l Joint Research] Research Institute for Tropical Medicine(Philippines)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Research Institute for Tropical Medicine(Philippines)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Differences in viral load among human respiratory syncytial virus genotypes in hospitalized children with severe acute respiratory infections in the Philippines2016

    • Author(s)
      Kadji FM, Okamoto M, Furuse Y, Tamaki R, Suzuki A, Lirio I, Dapat C, Malasao R, Saito M, Pedrera-Rico GA, Tallo V, Lupisan S, Saito M, Oshitani H.
    • Journal Title

      Virol J

      Volume: 13 Issue: 1 Pages: 113-113

    • DOI

      10.1186/s12985-016-0565-8

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Complete genome analysis of a rare G12P[6] rotavirus isolated in Thailand in 2012 reveals a prototype strain of DS-1-like constellation2016

    • Author(s)
      Saikruang W, Khamrin P, Malasao R, Kumthip K, Ushijima H Maneekarn N
    • Journal Title

      Virus Res

      Volume: 224 Pages: 38-45

    • DOI

      10.1016/j.virusres.2016.08.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Molecular Characterization of Human Respiratory Syncytial Virus in the Philippines, 2012-20132015

    • Author(s)
      Malasao R, Okamoto M, Chaimongkol N, Imamura T, Tohma K, Dapat I, Dapat C, Suzuki A, Saito M, Saito M, Tamaki R, Pedrera-Rico GA, Aniceto R, Quicho RF, Segubre-Mercado E, Lupisan S, Oshitani H.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 11 Pages: e0142192-e0142192

    • DOI

      10.1371/journal.pone.0142192

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] SAffold viruses in pediatric patients with diarrhea in Thailand2015

    • Author(s)
      Yodmeeklin A, Khamrin P, Chuchaona W, Saikruang W, Malasao R, Chaimongkol N, Kongsricharoern T, Ukarapol N, Maneekarn N
    • Journal Title

      J Med Virol

      Volume: 87 Issue: 4 Pages: 702-7

    • DOI

      10.1002/jmv.24114

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Molecular epidemiology of HRSV in Biliran, Philippines2016

    • Author(s)
      Malasao R, Okamoto M, Furuse Y, Dapat C, Saito M, Saito-Obata M, Tamaki R, Pedrera-Rico GA, Segubre-Mercado E, Lupisan S, Oshitani H
    • Organizer
      The 64th Annual Meeting of the Japanese Society for Virology
    • Place of Presentation
      Sapporo Convention Center, Sapporo(Japan)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Diversity of Complete Genome of Human Respiratory Syncytial Virus Subgroup A Isolated in The Philippines2015

    • Author(s)
      Malasao R, Furuse Y, Okamoto M, Dapat C, Dapat I, Suzuki A, Saito M, Saito M, Tamaki R, Pedrera-Rico GAG, Aniceto R, Quicho RFN, Segubre-Mercado E, Lupisan S, Oshitani H
    • Organizer
      1st International Meeting on Respiratory Pathogens (IMRP)
    • Place of Presentation
      Singapore(Singapore)
    • Year and Date
      2015-09-02
    • Related Report
      2015 Annual Research Report
  • [Remarks] 東北大学大学院医学系研究科 微生物学分野

    • URL

      http://www.virology.med.tohoku.ac.jp/

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Remarks] Tohoku-RITM Collaborating Research Center

    • URL

      http://www.virology.med.tohoku.ac.jp/project/jgrid/jgrid.html

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report

URL: 

Published: 2015-11-26   Modified: 2024-03-26  

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