| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
ATP-binding cassette (ABC) transporters constitute a large superfamily of membrane proteins that transport various substrates through membranes utilizing the energy of ATP. In this study, we investigated asymmetric dynamics of ABC transporters TM287/288 and CFTR induced by the binding of ATP and substrate using molecular simulations. It was revealed that substrate binding induced allosteric interdomain communication in TM287 / 288 and that the most frequent mutation in CFTR caused the disruption of a hydrophobic cluster at the domain interface, leading to dysfunction. These findings would provide important insights into the structure-function relationship of ABC transporters.
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