Development of a method for predicting functional sites in function-unknown splicing isoforms
| Project/Area Number |
15K00411
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Life / Health / Medical informatics
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| Research Institution | Nagahama Institute of Bio-Science and Technology |
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Principal Investigator |
Shionyu Masafumi 長浜バイオ大学, バイオサイエンス学部, 准教授 (30345847)
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| Co-Investigator(Kenkyū-buntansha) |
土方 敦司 長浜バイオ大学, バイオサイエンス学部, プロジェクト特任講師 (80415273)
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| Research Collaborator |
TEERASETMANAKUL Pramote
NAKATA Keisuke
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 選択的スプライシング / 機能未知タンパク質 / 機能部位予測 / 低分子化合物 / SNV / データベース / 機械学習 |
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| Outline of Final Research Achievements |
At present, many sequence data of splicing isoforms produced by alternative splicing are known. However, most of these splicing isoforms are function-unknown. In addition, it is argued whether they are functional or not. Thus, we developed a prediction method for functionality of splicing isoforms. Using our method, 70% of splicing isoforms, of which protein expression were not confirmed, were predicted to be functional, and this value is larger than one shown before. Moreover, we developed a method for predicting functional sites of splicing isoforms predicted to be functional. The method we developed showed higher accuracy for predicting small molecule-binding sites than that of our previous method. And we developed a method for predicting a small molecule ligand of a target protein that showed better performance than that of conventional one.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Ridaifen-F conjugated with cell-penetrating peptides inhibits intracellular proteasome activities and induces drug-resistant cell death.2018
Author(s)
Tanaka, M., Zhu, Y., Shionyu, M., Ota, N., Shibata, N., Watanabe, C., Mizusawa, A., Sasaki, R., Mizukami, T., Shiina, I., Hasegawa, M.
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Journal Title
European Journal of Medicinal Chemistry
Volume: 146
Pages: 636-650
DOI
Related Report
Peer Reviewed
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[Journal Article] The role of the Prod1 membrane anchor in newt limb regeneration2017
Author(s)
Nomura, K., Tanimoto, Y., Hayashi, F., Harada, E., Shan, X.-Y., Shionyu, M., Hijikata, A., Shirai, T., Morigaki, K., Shimamoto, K.
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Journal Title
Angew. Chem. Int. Ed.
Volume: 56
Issue: 1
Pages: 270-274
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of a High-Frequency Somatic NLRC4 Mutation as a Cause of Autoinflammation by Pluripotent Cell-Based Phenotype Dissection.2017
Author(s)
Kawasaki Y, Oda H, Ito J, Niwa A, Tanaka T, Hijikata A, Seki R, Nagahashi A, Osawa M, Asaka I, Watanabe A, Nishimata S, Shirai T, Kawashima H, Ohara O, Nakahata T, Nishikomori R, Heike T, Saito MK.
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Journal Title
Arthritis Rheumatol.
Volume: 69
Issue: 2
Pages: 447-459
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Molecular mechanisms of insulin resistance in 2 cases of primary insulin receptor defect-associated diseases.2017
Author(s)
Tsuji-Hosokawa, A., Takasawa, K., Nomura, R., Miyakawa, Y., Numakura, C., Hijikata, A., Shirai, T., Ogawa, Y., Kashimada, K. & Morio, T.
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Journal Title
Pediatr Diabetes
Volume: Feb 9
Issue: 8
Pages: 917-924
DOI
Related Report
Peer Reviewed
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