Mathematical Understanding of lnformation Processing Mechanism in the Brain by lnteraction of Glial and Neuronal Networks

• Project/Area Number: 15K00417
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Life / Health / Medical informatics
• Research Institution: The Institute of Statistical Mathematics
• Principal Investigator: Tamura Yoshiyasu 統計数理研究所, データ科学研究系, 教授 (60150033)
• Co-Investigator(Kenkyū-buntansha): 染谷 博司 東海大学, 情報理工学部, 准教授 (00333518) ; 越久 仁敬 兵庫医科大学, 医学部, 教授 (20252512) ; 岡田 泰昌 独立行政法人国立病院機構村山医療センター(臨床研究部), 電気生理学研究室, 室長 (80160688)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アストロサイト / カルシウムイメージング / 呼吸リズム / ニューロン / グリア細胞 / カルシームイメージング / 低酸素呼吸応答

Outline of Final Research Achievements

Astrocytes in the brain stem have an important role in respiratory rhythm formation in coordination with neurons. It was found that astrocytes play an important role in hypoxic respiratory response mechanism as hypoxic sensors in the brain.
We developed a simulation model by coupling a neuronal network model and an astrocytic oscillator model. We attempted to reveal roles of astrocytes in respiratory rhythm formation by simulations.

Research Products

• [Journal Article] Possible involvement of monoamine neurons in the emotional abnormality in Kir6.2-deficient mice (2018)
  • Author(s): Saito Atsumi、Miyagawa Kazuya、Miyagishi Hiroko、Kurokawa Kazuhiro、Umeda Akira、Okada Yasumasa、Tsuji Minoru、Takeda Hiroshi
  • Journal Title: Physiology & Behavior Volume: 188 Pages: 251-261
  • DOI: 10.1016/j.physbeh.2018.02.014
  • Peer Reviewed
• [Journal Article] Stochastic modeling for neural spiking events based on fractional superstatistical Poisson process (2018)
  • Author(s): H. Konno and Y. Tamura
  • Journal Title: AIP ADVANCES Volume: 8 Issue: 1 Pages: 0200131-6
  • DOI: 10.1063/1.5012547
  • Peer Reviewed / Open Access
• [Journal Article] The respiratory control mechanisms in the brainstem and spinal cord: integrative views of the neuroanatomy and neurophysiology. (2017)
  • Author(s): Ikeda K, Kawakami K, Onimaru H*, Okada Y, Yokota S, Koshiya N, Oku Y, Iizuka M, Koizumi H.
  • Journal Title: J Physiol Sci Volume: 67(1) Issue: 1 Pages: 45-62
  • DOI: 10.1007/s12576-016-0475-y
  • NAID: 40021064542
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 低酸素換気応答におけるアストロサイトの役割 (2017)
  • Author(s): 福士勇人, 堀内城司, 岡田泰昌
  • Journal Title: 自律神経 Volume: 54 Pages: 109-117
  • Peer Reviewed
• [Journal Article] A computational model of the respiratory network challenged and optimized by data from optogenetic manipulation of glycinergic neurons (2017)
  • Author(s): Oku Y, Hulsmann S.
  • Journal Title: Neuroscience Volume: 347 Pages: 111-122
  • DOI: 10.1016/j.neuroscience.2017.01.041
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Emergent Network Topology within the Respiratory Rhythm-Generating Kernel Evolved In Silico (2016)
  • Author(s): Amit Lal, Yoshitaka Oku, Hiroshi Someya, Fumikazu Miwakeichi, Yoshiyasu Tamura
  • Journal Title: PLoS One Volume: 6;11(5) Issue: 5 Pages: e0154049-e0154049
  • DOI: 10.1371/journal.pone.0154049
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Disharmony between wake- and respiration-promoting activities: Effects of modafinil on ventilatory control in rodents (2016)
  • Author(s): Terada J, Fukushi I, Takeda K, Hasebe Y, Pokorski M, Tatsumi K, Okada Y
  • Journal Title: Respiratory Research Volume: 17 Issue: 1 Pages: 148-148
  • DOI: 10.1186/s12931-016-0466-9
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Respiratory toxicity of dimethyl sulfoxide (2016)
  • Author(s): Takeda K, Pokorski M, Sato Y, Oyamada Y, Okada Y.
  • Journal Title: Adv Exp Med Biol. Volume: 885 Pages: 89-96
  • DOI: 10.1007/5584_2015_187
  • ISBN: 9783319258515, 9783319258539
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Respiratory calcium fluctuations in low-frequency oscillating astrocytes in the pre-Botzinger (2015)
  • Author(s): Y. Oku, J. Fresemann, F. Miwakeichi, S. Holsmann
  • Journal Title: Respiratory Physiology & Neurobiology Volume: 00 Pages: 00-00
  • DOI: 10.1016/j.resp.2015.02.002
  • Peer Reviewed / Open Access
• [Journal Article] Effects of arundic acid, an astrocytic modulator, on the cerebral and respiratory functions in severe hypoxia (2015)
  • Author(s): Fukushi I, Takeda K, Yokota S, Hasebe Y, Sato Y, Pokorski M, Horiuchi J, Okada Y.
  • Journal Title: Respir Physiol Neurobiol. Volume: S1569-9048 Pages: 30084-7
  • DOI: 10.1016/j.resp.2015.11.011
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Functional and morphological analysis of inhibitory neurons in the ventrolateral nucleus of the solitary tract on respiratory control (2018)
  • Author(s): Shigefumi Yokota, Kotaro Takeda, Yasumasa Okada, Yosuke Arima, Yukihiko Yasui, Nancy L Chamberlin
  • Organizer: 第123回日本解剖学会総会・全国学術集会
• [Presentation] 摘出脳幹標本におけるニューロンとアストロサイトの呼吸性活動：カルシウムイメージングによる解析 (2017)
  • Author(s): 岡田泰昌, 矢澤格, 武田湖太郎, 岡崎俊太郎, 田村義保, 鬼丸洋
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松
  • Year and Date: 2017-03-28
• [Presentation] ミトコンドリアのエネルギー産生モデルにおけるパラメータ推定 (2017)
  • Author(s): 田野 幹樹, 染谷 博司
  • Organizer: 第44回知能システムシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2017-03-13
• [Presentation] Role of glial cells in cardiorespiratory regulation (2017)
  • Author(s): Okada Y
  • Organizer: International Society for Autonomic Neuroscience Meeting (ISAN 2017)
  • Int'l Joint Research
• [Presentation] Tetanic stimulation of the hypothalamus evokes persistent excitation in the ventrolateral medulla: Spatiotemporal dynamics analyzed by voltage-sensitive dye imaging (2017)
  • Author(s): Y Kono, H Onimaru, I Fukushi, S Okazaki, S Yokota, K Takeda, Y Hasebe, K Koizumi, K Sugita, Y Okada
  • Organizer: International Society for Autonomic Neuroscience Meeting (ISAN 2017)
  • Int'l Joint Research
• [Presentation] Activated astrocytes induce persistence of post-stress blood pressure elevation (2017)
  • Author(s): Y Hasebe, S Sugama, K Takeda, K Koizumi, S Yokota, I Fukushi, M Hoshiai, Y Kakinuma, M Pokorski, J Horiuchi, K Sugita, Y Okada
  • Organizer: International Society for Autonomic Neuroscience Meeting (ISAN 2017)
  • Int'l Joint Research
• [Presentation] Respiratory modulation of astrocytes in the ventrolateral medulla of the isolated brainstem-spinal cord of the neonatal rat (2017)
  • Author(s): Okada Y, Yazawa I, Okazaki S, Yokota S, Takeda K, Someya H, Tamura Y, Onimaru H
  • Organizer: XIV Oxford Conference on Modelling and Control of Breathing
  • Int'l Joint Research
• [Presentation] Respiration-coupled rhythmic activity in the hypothalamus detected in the diencephalon-lower brainstem-spinal cord preparation (2017)
  • Author(s): Fukushi I, Kono Y, Yokota S, Okazaki S, Takeda K, Onimaru H, Okada Y
  • Organizer: XIV Oxford Conference on Modelling and Control of Breathing
  • Int'l Joint Research
• [Presentation] Detection of respiratory modulated astrocytes in the ventrolateral medulla of the isolated brainstem-spinal cord by confocal calcium imaging (2017)
  • Author(s): Okada Y, Yazawa I, Okazaki S, Yokota S, Takeda K, Someya H, Tamura Y, Onimaru H
  • Organizer: Annual Meeting of the Society for Neuroscience (Neuroscience2017)
  • Int'l Joint Research
• [Presentation] Rhythmic activity of astrocytes synchronized with alternating motor output during fictive locomotion (2017)
  • Author(s): Yazawa I, Okazaki S, Yokota S, Takeda K, Mikami H, Fukushi I, Onimaru H, Okada Y
  • Organizer: Annual Meeting of the Society for Neuroscience (Neuroscience2017)
  • Int'l Joint Research
• [Presentation] Post-stimulus persistent activation of the ventrolateral medulla in response to tetanic electrical stimulation of the hypothalamus analyzed by voltage imaging (2017)
  • Author(s): Kono Y, Onimaru H, Fukushi I, Okazaki S, Yokota S, Takeda K, Hasebe Y, Koizumi K, Sugita K, Okada Y
  • Organizer: Annual Meeting of the Society for Neuroscience (Neuroscience2017)
  • Int'l Joint Research
• [Presentation] General pattern of activation sequence among excitatory/inhibitory inspiratory neurons during rhythmic burst in the pre-Botzinger complex of the mice medulla slice (2017)
  • Author(s): 2.Oke Y, Miwakeichi F, Oku Y, Besser S, Hirrlinger J, Hulsmann S
  • Organizer: XIV Oxford Conference on Modelling and Control of Breathing
  • Int'l Joint Research
• [Presentation] Blockade of astrocytic activation delays the occurrence of severe hypoxia-induced seizure and respiratory arrest (2017)
  • Author(s): Fukushi I, Takeda K, Pokorski M, Horiuchi J, Okada Y
  • Organizer: 第57回日本呼吸器学会学術講演会
• [Presentation] 視床下部電気刺激で惹起される延髄腹側部の興奮：膜電位イメージングによる時空間ダイナミクス解析 (2017)
  • Author(s): 河野洋介, 鬼丸洋, 岡崎俊太郎, 武田湖太郎, 福士勇人, 小泉敬一, 星合美奈子, 杉田完爾, 岡田泰昌
  • Organizer: 第40回日本神経科学大会
• [Presentation] マウス孤束核腹外側亜核の抑制性ニューロンによる呼吸調節 (2017)
  • Author(s): 横田茂文, 武田湖太郎, 岡田泰昌, 有馬陽介, 浅野裕彦, 安井幸彦
  • Organizer: 第40回日本神経科学大会
• [Presentation] アストロサイト活性化抑制剤arundic acidは厳しい低酸素曝露によるけいれん発生と死を遅らせる (2017)
  • Author(s): 福士勇人, 武田湖太郎, 堀内城司, 岡田泰昌
  • Organizer: 第40回日本神経科学大会
• [Presentation] 遺伝的アルゴリズムを用いた画像認識によるマークシート自動採点システム (2017)
  • Author(s): 染谷博司、岡田泰昌、越久仁敬、田村義保
  • Organizer: 平成29年電気学会 電子・情報・システム部門大会
• [Presentation] 分子シミュレーションによるミトコンドリアのエネルギー産生モデル (2016)
  • Author(s): 田野 幹樹, 染谷 博司
  • Organizer: 第26回インテリジェント・システム・シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2016-10-27
• [Presentation] Hub structure emerges when network is evolved based on synchronicity.（ネットワークを同期性を基準に進化させるとハブ構造が出現する） (2016)
  • Author(s): Oku Y, Lal A, Someya H, Miwakeichi F, Tamura Y.
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] 低酸素換気応答におけるアストロサイトの役割 (2015)
  • Author(s): 福士勇人、武田湖太郎、長谷部洋平、堀内城司、岡田泰昌
  • Organizer: 第68回日本自律神経学会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-29
• [Presentation] A novel model of respiratory rhythm generation: a mechanism by interaction of intrinsically oscillating astrocytes and neurons (2015)
  • Author(s): Okada Y, Oku Y, Sasaki T, Vivar C, Yokota S, Takeda K, Fukushi I, Yazawa I, Someya H, Tamura Y
  • Organizer: Neuroscience 2015, 45th Society for Neuroscience Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Orexinergic fibers are in contact with Kolliker-Fuse neurons projecting to the rostral ventral respiratory group and phrenic and hypoglossal nuclei in the rat (2015)
  • Author(s): Yokota S, Okada Y, Oka T, Asano H, Yasui Y
  • Organizer: Neuroscience 2015, 45th Society for Neuroscience Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Maintenance of ventilation under a hypoxic condition requires activated astrocytes (2015)
  • Author(s): Fukushi I, Takeda K, Hasebe Y, Horiuchi J, Okada Y
  • Organizer: The International Society for Autonomic Neuroscience
  • Place of Presentation: Stresa, Italy
  • Year and Date: 2015-09-26
  • Int'l Joint Research
• [Presentation] Synchronized astrocytic network activity in the respiratory rhythm-generating kernel.（呼吸中枢におけるアストロサイトネットワークの同期活動） (2015)
  • Author(s): Oku Y，Miwakeichi F, Hulsmann S.
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-07-28
• [Presentation] Differentiation of neurons and astrocyte in brain stem by statistical image processing methods（統計的画像解析による脳幹のニューロン、アストロサイトの識別） (2015)
  • Author(s): Miwakeichi F, Oke Y, Oku Y.
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-07-28
• [Presentation] ストレス後の持続的な血圧上昇は活性化アストロサイトを介する (2015)
  • Author(s): 長谷部洋平、岡田泰昌、喜瀬広亮、戸田孝子、小泉敬一、杉田完爾、星合美奈
  • Organizer: 第51回日本小児循環器学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-16
• [Presentation] Role of TRP channels in respiratory control (2015)
  • Author(s): Okada Y, Takeda K, Oku Y, Onimaru H, Pokorski M
  • Organizer: Internatonal Meeting, TRPs and SOCs ~Unconventional Ca2+ Physiology~.
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2015-06-04
  • Int'l Joint Research / Invited
• [Presentation] Activated Astrocyte is Necessary in the Maintenance of the Higher Brain Function and Ventilation under a Hypoxic Condition. (2015)
  • Author(s): Fukushi I, Takeda K, Hasebe Y, Horiuchi J, Okada Y.
  • Organizer: 第55回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2015-04-17