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Denitrification, anammox, and DNRA in the hypereutrophic tidal flat: Influences of the dissolved organic matter (DOM) quantity and quality

Research Project

Project/Area Number 15K00525
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental dynamic analysis
Research InstitutionToho University

Principal Investigator

SENGA Yukiko  東邦大学, 理学部, 准教授 (30434210)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords嫌気的硝酸還元過程 / 脱窒 / アナモックス / DNRA / 大型海藻 / 干潟 / 硝酸還元過程 / 富栄養化 / 栄養塩 / アンモニアへの異化型硝酸還元 / 微生物的窒素浄化過程
Outline of Final Research Achievements

An increase in available nitrogen (NO3-) loading in intertidal ecosystems causes eutrophication and macroalgae blooms. In intertidal ecosystems subjected to excessive loading of NO3-, it is necessary to understand denitrification and anammox which are microbially mediated N removal processes as N2, and DNRA which conserves nitrogen in the sediment as NH4+. However, detailed information regarding these processes is not fully available. In the present study, we investigated 1) the contributions of denitrification and anammox for NO3- removal, 2) the development of sequential method to measure denitrification, anammox and DNRA, 3) the influences of macroalgae on denitrification, anammox and DNRA. Field investigation and sampling were performed in the hypereutrophic Yatsu tidal flat (Chiba Prefecture). The results suggest that the occurrence of macroalgae changes the progress of NO3- removal processes in intertidal ecosystems.

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は,脱窒,アナモックス,DNRAの同時測定法の確立に成功し,これまで不明とされていた富栄養化が著しい干潟におけるNO3-の動態が明らかになった点である.本研究により,富栄養化した干潟においてNO3-は主に脱窒を介して除去されること,大型藻類の出現はNO3-除去過程を大きく左右することといった2点の新知見を提示することができた.
近年,世界中の沿岸域において富栄養化が問題となっており,水質を改善するための対策や窒素浄化の技術開発の確立が喫緊の課題となっている.本研究の成果は,そのような対策や技術開発に基礎的知見を提供するものであり,これが本研究の社会的意義である.

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report

Research Products

(24 results)

All 2019 2018 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 3 results,  Open Access: 2 results) Presentation (14 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] Anammox and denitrification in the intertidal sediment of the hypereutrophic Yatsu tidal flat, Japan.2019

    • Author(s)
      Senga Y, Sato T, Kuroiwa M, Nohara S, Suwa Y
    • Journal Title

      Estuaries and Coasts

      Volume: 42 Pages: 665-674

    • DOI

      10.1007/s12237-019-00520-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of parasitic chytrids on the quantity and quality of algal dissolved organic matter (AOM)2018

    • Author(s)
      Senga Yukiko、Yabe Shiori、Nakamura Takaki、Kagami Maiko
    • Journal Title

      Water Research

      Volume: 145 Pages: 346-353

    • DOI

      10.1016/j.watres.2018.08.037

    • NAID

      120006540109

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterizing the transformation of aquatic humic substances exposed to ultraviolet radiation using excitation-emission matrix fluorescence spectroscopy and PARAFAC2018

    • Author(s)
      Senga Y, Naruoka C, Moriai S, Nohara S
    • Journal Title

      Inland Waters

      Volume: 8 Pages: 505-511

    • DOI

      10.1080/20442041.2018.1506650

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A method for determining Batillaria attramentaria distribution using aerial balloon photography and a vegetation index camera: Demonstration at the Yatsu Tidal Flat, Chiba Prefecture.2018

    • Author(s)
      Shiraki Y, Kitazawa T, Lee S, Senga Y
    • Journal Title

      Advances in remote sensing

      Volume: 7 Pages: 15-24

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sensitive Method for the Oxidation-determination of Trace Hydroxylamine in Environmental Water Using Hypochlorite Followed by Gas Chromatography2017

    • Author(s)
      Kato T, Sugahara S, Murakami M, Senga Y, Egawa M, Omata K, Seike Y
    • Journal Title

      Anal. Sci.

      Volume: 33 Issue: 6 Pages: 691-695

    • DOI

      10.2116/analsci.33.691

    • NAID

      130005696780

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterizing the photochemical degradation of aquatic humic substances from a dystrophic lake using excitation-emission matrix fluorescence spectroscopy and parallel factor analysis.2017

    • Author(s)
      Senga Y, Moriai S, Naruoka C, Nedachi R, Terui S
    • Journal Title

      Limnology

      Volume: 18 Pages: 97-110

    • DOI

      10.1007/s10201-016-0493-8

    • NAID

      40021062519

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Determination of Trace Hydrazine in Environmental Water Samples by <i>in situ</i> Solid Phase Extraction2017

    • Author(s)
      Kato T, Sugahara S, Kajitani T, Senga Y, Egawa M, Kamiya H, Seike Y
    • Journal Title

      Anal. Sci.

      Volume: 33 Issue: 4 Pages: 487-491

    • DOI

      10.2116/analsci.33.487

    • NAID

      130005587974

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Variation in microbial function through soil depth profiles in the Kushiro Wetland, northeastern Hokkaido, Japan.2015

    • Author(s)
      Senga Y., Hiroki M., Terui S., Nohara S.
    • Journal Title

      Ecological Research

      Volume: 30 Pages: 1-10

    • DOI

      10.1007/s11284-015-1257-3

    • NAID

      40020539395

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Influence of hypersaturated dissolved oxygenated water on the elution of hydrogen sulfide and methane from sediment in the dredged area in polyhaline Lake Nakaumi2015

    • Author(s)
      Sugahara S, Kamiya H, Suyama Y, Senga Y, Ayukawa K, Okumura M, Seike Y
    • Journal Title

      Landscape and Ecological Engineering

      Volume: 11 Pages: 269-282

    • DOI

      10.1007/s11355-014-0270-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 尾瀬ヶ原池溏群を用いた水系腐植物質の特性解析2018

    • Author(s)
      1)千賀有希子,熊崎悠一,成岡知佳,野原精一
    • Organizer
      第65回日本生態学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Microbial function and chemical properties through a wetland soil depth profiles2017

    • Author(s)
      Yukiko Senga, Chika Naruoka, Seiichi Nohara
    • Organizer
      ASLO
    • Place of Presentation
      Hawaii Convention Center, Honolulu, USA
    • Year and Date
      2017-03-02
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Tracing the transformations of aquatic humic substances in bog water using EEM-PARAFAC2017

    • Author(s)
      Chika Naruoka, Yukiko Senga
    • Organizer
      ASLO
    • Place of Presentation
      Hawaii Convention Center, Honolulu, USA
    • Year and Date
      2017-02-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 谷津干 潟におけるグリーンタイドと微生物活性2017

    • Author(s)
      千賀有希子,北村 友佳,王川 香澄,柴木 叶,小林 渉,佐藤 翼,成岡 知佳,北沢俊幸,李盛源,白木洋平
    • Organizer
      日本陸水学会第82回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 谷津干潟堆積物における脱窒とアナモックスの集積培養2017

    • Author(s)
      佐藤翼,諏訪裕一,黒岩恵,千賀有希子
    • Organizer
      日本陸水学会第82回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 谷津干潟堆積物における脱窒とアナモックス過程2016

    • Author(s)
      佐藤翼,諏訪裕一,野原精一,千賀有希子
    • Organizer
      日本陸水学会第81回大会
    • Place of Presentation
      琉球大学(沖縄県那覇市)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Research-status Report
  • [Presentation] 尾瀬ヶ原池塘における水系腐植物質の動態解析2016

    • Author(s)
      成岡知佳,千賀有希子
    • Organizer
      日本陸水学会第81回大会
    • Place of Presentation
      琉球大学(沖縄県那覇市)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Research-status Report
  • [Presentation] 尾瀬ヶ原池溏における水系腐植物質の動態解析2016

    • Author(s)
      熊崎悠一,成岡知佳,野原精一,千賀有希子
    • Organizer
      日本陸水学会第81回大会
    • Place of Presentation
      琉球大学(沖縄市那覇市)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Research-status Report
  • [Presentation] Photochemical degradation of aquatic humic substances traced by EEM-PARAFAC analysis2016

    • Author(s)
      Yukiko Senga, Chika Naruoka, Satoshi Moriai
    • Organizer
      INTECOL Wetlands
    • Place of Presentation
      Changshu International Conference Center, Changshu, China
    • Year and Date
      2016-09-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ツボカビの寄生が珪藻Synedra から産生する溶存有機物に与える影響-三次元励起蛍光スペクトル(EEM)-PARAFAC法を用いた解析-2016

    • Author(s)
      矢部詩織, 中村昂樹, 鏡味麻衣子, 千賀有希子
    • Organizer
      第63回日本生態学会大会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] 谷津干潟におけるグリーンタイド現象と水質2015

    • Author(s)
      千賀有希子,根立竜馬,盛合哲史,山内翔平,和田憲昭,近藤拓未,宮田勉,森朱音,佐藤翼,成岡知佳,矢部詩織,北沢俊幸,李盛源,白木洋平
    • Organizer
      日本陸水学会第80回大会
    • Place of Presentation
      北海道大学函館キャンパス(北海道函館市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
  • [Presentation] 谷津干潟におけるグリーンタイド現象が微生物機能に与える影響2015

    • Author(s)
      根立竜馬,佐藤翼,千賀有希子
    • Organizer
      日本陸水学会第80回大会
    • Place of Presentation
      北海道大学函館キャンパス(北海道函館市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
  • [Presentation] 三次元励起蛍光スペクトル(EEM)- PARAFAC法を用いた尾瀬ヶ原池溏の溶存有機物の解析2015

    • Author(s)
      成岡知佳,野原精一,千賀有希子
    • Organizer
      日本陸水学会第80回大会
    • Place of Presentation
      北海道大学函館キャンパス(北海道函館市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
  • [Presentation] 三次元励起蛍光スペクトル(EEM)-PARAFAC法を用いたChlorella sp.およびNitzschia palea産生有機物の特性解析2015

    • Author(s)
      森朱音,千賀有希子
    • Organizer
      日本陸水学会第80回大会
    • Place of Presentation
      北海道大学函館キャンパス(北海道函館市)
    • Year and Date
      2015-09-28
    • Related Report
      2015 Research-status Report
  • [Book] 陸水環境化学2017

    • Author(s)
      藤永薫[編集],大嶋俊一,管原庄吾,杉山裕子,千賀有希子,藤永薫,向井浩,山田佳裕
    • Total Pages
      131
    • Publisher
      共立出版
    • ISBN
      9784320047334
    • Related Report
      2017 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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