Analysis of secondary DNA damage elicited by nucleotide excision repair and the genome maintenance mechanism in mammalian quiescent cells
| Project/Area Number |
15K00534
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松永 司 金沢大学, 薬学系, 教授 (60192340)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ヌクレオチド除去修復 / DNA損傷応答 / 二次的DNA損傷 / ssDNAギャップ / DNA二本鎖切断 |
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| Outline of Final Research Achievements |
In quiescent cells, nucleotide excision repair (NER) process generates multiple types of secondary DNA damage. However, the mechanism of secondary DNA damage formation and their biological meanings are unclear. In this study, we have examined the possible involvement of exonuclease 1 (Exo1) in the processing of ssDNA gap intermediate during NER. We found that Exo1 plays an important role in the processing of NER-induced ssDNA gap intermediates and protects UV-induced cell death in quiescent cells. In addition, we could detect several NER-dependent DNA damage responses in mouse skin, such as H2AX phosphorylation and ATM activation, indicating that NER-dependent formation of secondary DNA damage indeed occurs in vivo as well.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Rapid G0/1 transition and cell cycle progression in CD8+ T Cells compared to CD4+ T Cells following in vitro stimulation.2017
Author(s)
Mishima, T., Fukaya, S., Toda, S., Ando, Y., Matsunaga, T. and Inobe, M.
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Journal Title
Microbiology and Immunology
Volume: 印刷中
NAID
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Peer Reviewed
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[Presentation] A small molecule inhibitor of nucleotide excision repair from chemical library screening using a newly developed cell-based immunoassay2015
Author(s)
Nishinaga,M., Miyazaki, K., Takamori, C., Ohzawa, T., Wakasugi, M., Saito, T., Osada, H. and Matsunaga, T.
Organizer
The 15th International Congress of Radiation Research
Place of Presentation
国立京都国際会館（京都府京都市）
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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[Presentation] Activation of ATR and ATM signaling pathways by NER-dependent secondary damage in mammalian quiescent cells2015
Author(s)
Wakasugi, M., Sasaki, T., Matsumoto, M., Nagaoka, M., Inoue, K., Inobe, M., Horibata, K., Tanaka, K. and Matsunaga, T.
Organizer
The 15th International Congress of Radiation Research
Place of Presentation
国立京都国際会館（京都府京都市）
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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[Book] 光と生命の事典2016
Author(s)
日本光生物学協会 光と生命の事典編集委員会 編
Total Pages
436
Publisher
朝倉書店
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