| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In quiescent cells, nucleotide excision repair (NER) process generates multiple types of secondary DNA damage. However, the mechanism of secondary DNA damage formation and their biological meanings are unclear. In this study, we have examined the possible involvement of exonuclease 1 (Exo1) in the processing of ssDNA gap intermediate during NER. We found that Exo1 plays an important role in the processing of NER-induced ssDNA gap intermediates and protects UV-induced cell death in quiescent cells. In addition, we could detect several NER-dependent DNA damage responses in mouse skin, such as H2AX phosphorylation and ATM activation, indicating that NER-dependent formation of secondary DNA damage indeed occurs in vivo as well.
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