|Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|Outline of Final Research Achievements
Seven neonicotinoids were separately administered to female NZ white rabbits; and the urine samples were collected after 12-48 hours. More than 20 metabolites, such as hydroxy-imidacloprid, N-desmethyl-acetamiprid, N-desmethyl-colthianidin, N-desmetyl-nitenpyram, and N-desmetyl-dinotefuran, were qualitatively detected from the urine samples by LC-TOFMS.
We synthesized 13 metabolites, which were not commercially available, as standards substances and developed quantitative bioanalytical methods using LC-MS/MS.
Then we collected urine samples from the general population, e.g. 46 from three- to six-year-old children in Nagano; 102 from Sri Lankan people, some with chronic kidney disease; and 78 from people in Ghana. The following compounds, imidacloprid, acetamiprid, thiacloporid, nitenpyram, thiamethoxam, clothianidin, dinotefuran, hydroxy-imidacloprid, N-desmethyl-acetamiprid, N-desmethyl-colthianidin, and N-desmethyl-nitenpyram were quantitatively detected from those urine samples.