Development of a co-benefit type vegetative treatment system using the Lemnoideae with enhanced frond function

• Project/Area Number: 15K00580
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental engineering and reduction of environmental burden
• Research Institution: University of Yamanashi
• Principal Investigator: TANAKA Yasuhiro 山梨大学, 大学院総合研究部, 准教授 (50377587)
• Co-Investigator(Kenkyū-buntansha): 遠山 忠 山梨大学, 大学院総合研究部, 准教授 (60431392) ; 森 一博 山梨大学, 大学院総合研究部, 准教授 (90294040)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ウキクサ / PGPB / 水質浄化 / 植生浄化 / 水生植物 / 微生物 / 葉状体

Outline of Final Research Achievements

We succeeded in isolating two plant growth-promoting bacteria (PGPB; the strains LA-C6 and SP-2-C10) from Lemna minor, a member of Lemnoideae. These PGPB also elevated the growth of microalgae.
Additionally, 27 bacterial strains, which have a gene for phenol hydroxylase involving degradation of phenol and trichloroethylene etc., were isolated from the fronds of Lemnoideae. By inoculating the phenol hydroxylase gene positive strains, 21L25, 22L16, 22L21, 32L3 and 22L7, into aseptic duckweed, we successfully obtained the plant possessing high level of phenol degrading ability. During this process, it was also revealed that the strain 22L7 is a PGPB for L. minor.

Research Products

• [Journal Article] Isolation of Novel Bacteria Including Rarely Cultivated Phyla, <i>Acidobacteria</i> and <i>Verrucomicrobia</i>, from the Roots of Emergent Plants by Simple Culturing Method (2017)
  • Author(s): Tanaka, Y., H. Matsuzawa, H. Tamaki, M. Tagawa, T. Toyama, Y. Kamagata, and K. Mori
  • Journal Title: Microbes and Environments Volume: 32 Issue: 3 Pages: 288-292
  • DOI: 10.1264/jsme2.ME17027
  • NAID: 130006105353
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ウキクサ亜科植物と微生物間の相互作用を用いた新しい植生浄化プロセスの開発 (2017)
  • Author(s): 田中靖浩, 遠山忠, 森一博,玉木秀幸
  • Journal Title: アグリバイオ Volume: 1 Pages: 68-69
  • Acknowledgement Compliant
• [Journal Article] 未知微生物をお手軽に分離培養するテクニック (2016)
  • Author(s): 田中靖浩,玉木秀幸
  • Journal Title: The Chemical Times Volume: 239 Pages: 26-31
  • Peer Reviewed / Open Access
• [Journal Article] Zincmethylphyrins and coproporphyrins, novel growth factors released by Sphingopyxis sp., enable laboratory cultivation of previously uncultured Leucobacter sp. through interspecies mutualism (2016)
  • Author(s): Bhuiyan M.N., Takai R., Mitsuhashi S., Shigetomi K., Tanaka Y., Kamagata Y., Ubukata M.
  • Journal Title: The Journal of Antibiotics Volume: 69 Issue: 2 Pages: 97-103
  • DOI: 10.1038/ja.2015.87
  • NAID: 120005749616
  • Peer Reviewed / Open Access
• [Presentation] Fimbrimonadaceae科、Hyphomonadaceae科に属する新規細菌によるウキクサおよび微細藻類の生育促進 (2018)
  • Author(s): 岩下智貴、花岡翼、遠山忠、森一博、田中靖浩、玉木秀幸、米田恭子、牧野彩花、鎌形洋一、森川正章
  • Organizer: 第52回日本水環境学会年会
• [Presentation] ウキクサを釣り針とした難培養性細菌群の捕集と分離培養 (2018)
  • Author(s): 戸澤恵里奈、田中靖浩、遠山忠、森一博、玉木秀幸、米田恭子、牧野彩花、鎌形洋一、森川正章
  • Organizer: 第52回日本水環境学会年会
• [Presentation] ウキクサ亜科植物に接種したPGPBの葉状体および根における定着性の評価 (2017)
  • Author(s): 岩下智貴，田中靖浩，玉木秀幸，米田恭子，牧野彩花，遠山忠，鎌形洋一，森川正章，森一博
  • Organizer: 第51回日本水環境学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス（熊本県熊本市）
  • Year and Date: 2017-03-15
• [Presentation] Isolation of plant growth-promoting bacteria from cormosphere of duckweed (2017)
  • Author(s): Tomoki Iwashita, Yasuhiro Tanaka, Hideyuki Tamaki, Yasuko Yoneda, Ayaka Makino, Tadashi Toyama, Yoichi Kamagata, Masaaki Morikawa, Kazuhiro Mori
  • Organizer: The 5th International Young Researchers Workshop on River Basin Environment and Management
  • Int'l Joint Research
• [Presentation] 難培養性細菌群Armatimonadetes門に属する新規細菌LA-C6株によるウキクサ亜科植物の生育促進 (2016)
  • Author(s): 岩下智貴，田中靖浩，玉木秀幸，立野由佳，米田恭子，牧野彩花，遠山忠，鎌形洋一，森川正章，森一博
  • Organizer: 日本微生物生態学会第31回大会
  • Place of Presentation: 横須賀市文化会館（神奈川県横須賀市）
  • Year and Date: 2016-10-22
• [Presentation] 水生植物ウキクサからの難培養性細菌群Verrucomicrobia門細菌およびArmatimonadetes門細菌の分離培養 (2016)
  • Author(s): 戸澤恵里奈，田中靖浩，玉木秀幸，新村杏菜，米田恭子，牧野彩花，遠山忠，鎌形洋一，森川正章，森一博
  • Organizer: 日本微生物生態学会第31回大会
  • Place of Presentation: 横須賀市文化会館（神奈川県横須賀市）
  • Year and Date: 2016-10-22
• [Presentation] ウキクサの葉状体と根に集積するフェノール分解菌の分離と比較 (2016)
  • Author(s): 大内源樹，田中靖浩，玉木秀幸，新村杏菜，米田恭子，牧野彩花，遠山忠，鎌形洋一，森川正章，森一博
  • Organizer: 日本微生物生態学会第31回大会
  • Place of Presentation: 横須賀市文化会館（神奈川県横須賀市）
  • Year and Date: 2016-10-22
• [Presentation] ウキクサ亜科植物葉状体からの植物生育促進細菌の探索 (2016)
  • Author(s): 岩下智貴,田中靖浩,玉木秀幸,米田恭子,牧野彩花,遠山忠,鎌形洋一,森川正章,森一博
  • Organizer: 日本農芸化学会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-03-27
• [Presentation] ウキクサ亜科葉状体由来新規微生物コレクションからのPGPBの検索 (2015)
  • Author(s): 岩下智貴,田中靖浩,玉木秀幸,牧野彩花,遠山忠,鎌形洋一,森川正章,森一博
  • Organizer: 日本生物工学会
  • Place of Presentation: 城山観光ホテル（鹿児島県鹿児島市）
  • Year and Date: 2015-10-26
• [Remarks] 山梨大学環境保全研究室
  • URL: http://www.ccn.yamanashi.ac.jp/~5lab/
• [Remarks] 山梨大学生命環境学部環境科学科・田中研究室
  • URL: https://evtanaka.blogspot.jp/
• [Remarks] 山梨大学生命環境学部環境科学科・田中研究室
  • URL: http://evtanaka.blog.fc2.com/
• [Remarks] 山梨大学環境保全研究室ホームページ
  • URL: http://www.ccn.yamanashi.ac.jp/~5lab/