Behavior of Escherichia coli O157 via cooking utensils

• Project/Area Number: 15K00784
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: The University of Tokushima
• Principal Investigator: Yokoigawa Kumio 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 教授 (60230637)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 大腸菌O157 / 調理器具 / バイオフィルム / 付着性 / 食品衛生 / 二次汚染 / 大腸菌Ｏ１５７

Outline of Final Research Achievements

To suppress the food pollution by Escherichia coli O157 via cooking utensil, we examined the survival and resistance against disinfectant of the pathogen. The cells attached to the surfaces of cooking utensils lived for long time even in the tap water. The disinfectant-resistance of cells attached to the surfaces immediately increased after the initial attachment. Polysaccharides among food components markedly stimulated the initial attachment and biofilm-formation of the cells.

Research Products

• [Journal Article] Diverse cellular actions of tert-butylhydroquinone, a food additive, on rat thymocyte (2017)
  • Author(s): Norio Kamemura, Keisuke Oyama, Kaori Kanemaru, Kumio Yokoigawa and Yasuo Oyama
  • Journal Title: Toxicology Research Volume: 6 Issue: 6 Pages: 922-929
  • DOI: 10.1039/c7tx00183e
  • NAID: 120006524488
  • Peer Reviewed
• [Journal Article] Screening and analysis of edible seaweeds in the ability to adsorb Shiga toxin (2017)
  • Author(s): Badr Hoida Ali、Takahashi Keiko、Kawakami Ryushi、Oyama Yasuo、Yokoigawa Kumio、Kanemaru Kaori
  • Journal Title: European Food Research and Technology Volume: 243 Issue: 12 Pages: 2147-2153
  • DOI: 10.1007/s00217-017-2915-1
  • NAID: 120006470107
  • Peer Reviewed
• [Journal Article] Change in plasma membrane potential of rat thymocytes by tert-butylhydroquinone, a food additive: Possible risk on lymphocytes (2017)
  • Author(s): Maki Takeda, Keisuke Oyama, Norio Kamemura, Kaori Kanemaru, Keizo Yuasa, Kumio Yokoigawa and Yasuo Oyama
  • Journal Title: Food and Chemical Toxicology Volume: 109 Pages: 296-301
  • DOI: 10.1016/j.fct.2017.09.017
  • NAID: 120006510951
  • Peer Reviewed
• [Journal Article] 食品加工・調理器具への微生物の付着と殺菌剤耐性 (2016)
  • Author(s): 金丸 芳, 逵 牧子, 横井川 久己男
  • Journal Title: 食品機械装置 Volume: 53 Pages: 50-55
  • NAID: 120006763925
  • Peer Reviewed
• [Journal Article] Genotyping and characterisation of the secretory lipolytic enzymes of Malassezia pachydermatis isolates collected dogs. (2015)
  • Author(s): Hideshi Teramoto1, Yuko Kumeda, Kumio Yokoigawa, Koji Hosomi, Shunji Kozaki, Masafumi Mukamoto, and Tomoko Kohda
  • Journal Title: Vet Rec Open Volume: 2 Issue: 2 Pages: 1-9
  • DOI: 10.1136/vetreco-2015-000124
  • NAID: 120006875684
  • Peer Reviewed / Open Access
• [Presentation] 非生物素材に付着した大腸菌O157のバイオフィルム形成 (2017)
  • Author(s): 横井川 久己男, 高橋 啓子, 金丸 芳
  • Organizer: 日本農芸化学会2017大会
  • Place of Presentation: 京都女子大学（京都府・京都市）
  • Year and Date: 2017-03-19
• [Presentation] 調理器具素材への大腸菌の付着を抑制する小麦粉の成分について (2017)
  • Author(s): 酒井 仁美, 酒井 徹, 横井川 久己男
  • Organizer: 第38回日本食品微生物学会学術総会
• [Presentation] 非生物素材に付着した大腸菌O157のバイオフィルム形成 (2017)
  • Author(s): 横井川 久己男, 高橋 啓子, 金丸 芳 :
  • Organizer: 日本農芸化学会2017大会,
• [Presentation] 調理器具素材への大腸菌の付着に及ぼす穀類の影響 (2017)
  • Author(s): 酒井 仁美, 酒井 徹, 横井川 久己男 :
  • Organizer: 第37回日本食品微生物学会学術総会講演要旨集
• [Presentation] 食品加工・調理器具素材に対する付着とバイオフィルム形成 (2016)
  • Author(s): 高橋啓子、金丸芳、横井川久己男
  • Organizer: 第3回日本生物工学会西日本支部講演会
  • Place of Presentation: 徳島大学（徳島県・徳島市）
  • Year and Date: 2016-12-10
• [Presentation] 調理器具素材への大腸菌の付着に及ぼす穀類の影響 (2016)
  • Author(s): 酒井 仁美, 酒井 徹, 横井川 久己男
  • Organizer: 日本農芸化学会中四国支部第45回講演会
  • Place of Presentation: 香川大学（香川県・木田郡三木町）
  • Year and Date: 2016-06-11
• [Presentation] Adsorption of shiga toxin to foods containing indigestible components. (2015)
  • Author(s): Kumio Yokoigawa, Makiko Tsuji, Tsukie Goto
  • Organizer: Pacifichem2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-17
  • Int'l Joint Research