New Development of Foods for Preventing Vascular Diseases-Search for Biomarkers for Disease Prevention on the plasma Exosomes-
| Project/Area Number |
15K00809
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | Jissen Women's University (2018-2019)
Gunma University (2015-2017) |
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Principal Investigator |
Nakamura Akio 実践女子大学, 生活科学部, 教授 (30282388)
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| Co-Investigator(Kenkyū-buntansha) |
岸 博子 山口大学, 大学院医学系研究科, 准教授 (40359899)
河原田 律子 (那須律子) 高崎健康福祉大学, 健康福祉学部, 講師 (60383147)
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| Project Period (FY) |
2015-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 喫煙 / ニコチン / アテローム性動脈硬化症 / 血管平滑筋細胞 / 形質転換 / オミックス研究 / マイクロＲＮＡ / ヒト疾患モデル細胞 / ヒト血管平滑筋細胞 / 細胞分化・脱分化 / エクソソーム / プロテオーム / 細胞遊走 / マイクロRNA / 動脈硬化性疾患 / タバコ煙 / 網羅的遺伝子解析 / プロテオーム解析 / 脱分化 / 抗酸化物質 / 機能性食品 |
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| Outline of Final Research Achievements |
We constructed the model cell of arteriosclerosis by exposing human vascular smooth muscle cells to the nicotine contained in the mainstream of cigarette smoke. Using this human disease model cell, the identification of molecules involved in the transformation of vascular smooth muscle cells from contraction type to migration/proliferation type was carried out focusing on exosomes secreted from the vascular smooth muscle cells. At first, we established an isolation method of exosomes secreted from vascular smooth muscle cells from the culture supernatant. Secondary, we analyzed the micro RNAs and peptides contained in this exosome. As a result, we found some characteristic microRNAs and peptides. In the future, we would like to search for candidates for disease biomarkers among them and apply them to search for compounds that prevent arteriosclerosis from food ingredients.
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| Academic Significance and Societal Importance of the Research Achievements |
研究代表者は、喫煙において、タバコの主流煙に含まれるニコチンがアテローム性動脈硬化症の危険因子であり、このニコチンが血管平滑筋細胞を収縮型から増殖型に形質転換することでアテローム形成を促すことを明らかにした。近年、細胞が分泌するエクソソームと呼ばれる小胞に含まれる分子ががんの早期発見のための疾患バイオマーカーとして世界中で注目されている。我々は独自に構築したアテローム性動脈硬化症ヒト疾患モデル細胞を用いて、分泌されるエクソソームが運搬する分子をバイオマーカーとして利用し、動脈硬化性疾患に対して改善効果が期待されている機能性食品成分の科学的根拠を調べることで一次予防に貢献できると考えている。
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Report
(6 results)
Research Products
(55 results)
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[Journal Article] NOX1/NADPH Oxidase Expressed in Colonic Macrophages Contributes to the Pathogenesis of Colonic Inflammation in Trinitrobenzene Sulfonic Acid-Induced Murine Colitis2017
Author(s)
Yokota H, Tsuzuki A, Shimada Y, Imai A, Utsumi D, Tsukahara T, Matsumoto M, Amagase K, Iwata K, Nakamura A, Yabe-Nishimura C, Kato S
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Journal Title
The Journal of Pharmacology and Experimental Theraｐeutics
Volume: 360(1)
Issue: 1
Pages: 192-200
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Oral administration of Dictyostelium differentiation-inducing factor 1 lowers blood glucose levels in streptozotocin- induced diabetic rats.2016
Author(s)
Kawaharada, R., Nakamura, A., Takahashi, K., Kikuchi, H., Oshima, Y., and Kubohara, Y.
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Journal Title
Life Sci.
Volume: 155
Pages: 56-62
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Effects of AGEs on the rat neuronal cells in the intrauterine hyperglycaemic environment.2020
Author(s)
Nakamura, A., Okami, H., Shimizu, M., Ooi, S., Tokunaga, Y., Sasaki, Y., and Kawaharada, R.
Organizer
93rd Annual Meeting of The Japanese Pharmacological Society（Yokohama, Japan)
Related Report
Int'l Joint Research
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[Presentation] Intrauterine hyperglycemia induced the inflammatory signalling in the cardiac muscle of the infants of diabetic mother rats2017
Author(s)
Nakamura, A., Imai, M., Inoue, A., Okami H., Sato, A., Yano, A., Kawaharada, R.,
Organizer
10th World Congress on Development Origins of Health and Disease
Related Report
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