| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In the present study, we examined whether vitamin E affects wound-mediated HaCaT keratinocyte polarization process including the recruitment of Par3 and aPKC, leading to wound repair independently of its antioxidant activity. Functional assays showed that vitamin E but not other antioxidants enhanced wound closure as well as cell polarization, and regulated the localization and complex formation of Par3 and aPKC in HaCaT cells after wounding. Knockdown of aPKC or Par3 abrogated vitamin E-mediated promotion of the wound closure and cell polarization in HaCaT cells. We also found that aPKC kinase activity was significantly increased in vitamin E-treated cells through activation of phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway.
Our study has demonstrated that vitamin E promotes the cell polarization and migration in human keratinocytes HaCaT cells after wounding via PI3K/aPKC signaling cascade, resulting in acceleration of wound repair.
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