The study of nutrition starvation in Campylobacter jejuni infection for nutritional therapy
| Project/Area Number |
15K00819
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SHIMOHATA Takaaki 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (90609687)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Campylobacter jejuni / Autophagy / Amino acid / amino acid / mTOR / カンピロバクター / アミノ酸 / オートファジー |
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| Outline of Final Research Achievements |
Campylobacter jejuni is a major cause of bacterial hood borne illness worldwide. C. jejuni virulence is closely associated with entering into host epithelial cells.
Against to invasive bacteria, autophagy is activated for bacterial clearance. But it is not clear how autophagy effect on the bacteria survive in invading C. jejuni. Here we investigated the role of autophagy in C. jejuni-infection.
C. jejuni infection induced autophagy in host epithelial cells, but autophagy-associated proteins did not co-localized with C. jejuni. Additionally, inhibition of autophagy decreased bacterial survival and amino acid content in infected cells. The survival rate of intracellular bacteria were up-regulated by amino acid supplementation. Those data indicate that induction of autophagy could contribute to survive of C. jejuni in epithelial cells efficiently. Our data suggest that autophagy must be target of nutritional treatment in infection disease.
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Report
(4 results)
Research Products
(14 results)
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[Presentation] 宿主腸管上皮細胞のTight Junction形成が食中毒原因菌Campylobacter jejuniの侵入機構に及ぼす影響について2017
Author(s)
畑山 翔, 下畑 隆明, 天野 幸恵, 木戸 純子, 神田 結奈, 天宅 あや, 福島 志帆, 中橋 睦美, 上番増 喬, 馬渡 一諭, 髙橋 章
Organizer
第71回 日本栄養食糧学会
Related Report
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[Presentation] Campylobacter jejuni感染で誘導されるオートファジーは菌のクリアランス機構として働くのか2017
Author(s)
福島 志帆, 下畑 隆明, 畑山 翔, 木戸 純子, 天宅 あや, 神田 結奈, 鳴滝 涼香, 石田 快, 上番増 喬, 馬渡 一諭, 髙橋 章
Organizer
第38回 日本食品微生物学会学術総会
Related Report
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[Presentation] CFTR reduced microtubule-mediated Campylobacter jejuni invasion2017
Author(s)
木戸 純子, 下畑 隆明, 天野 幸恵, 畑山 翔, 佐藤 優里, 神田 結奈, 天宅 あや, 福島 志帆, 中橋 睦美, 上番増 喬, 馬渡 一諭, 髙橋 章
Organizer
日米医学協力研究会コレラ・細菌性腸管感染症専門部会
Related Report
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