Regulation and physiological significance of histamine production in Raoultella ornithinolytica

• Project/Area Number: 15K00822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Kagawa University
• Principal Investigator: Imaohji Haruyuki 香川大学, 医学部, 講師 (80294669)
• Co-Investigator(Kenkyū-buntansha): 桑原 知巳 香川大学, 医学部, 教授 (60263810)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Raoultella / ヒスタミン産生 / ヒスチジン / カタボライト抑制 / 酸素ストレス / R. ornithinolytica / ヒスタミン / hdc / recA / H2O2 / 腸内菌 / 腸管上皮細胞増殖 / ノトバイオート / histidine decarboxylase / crp / fis / ヒスタミン食中毒

Outline of Final Research Achievements

Raoultella orithinolytica is a member of Enterobacteriaceae, which widely distributes the environment including human intestine. In this study, we revealed the mechanism of histamine production in R. ornithinolytica strain AA097. We found that histamine production in R. orithinolytica is under coordinate positive regulation by CRP and Fis. The histamine-producing R. orithinolytica stimurated colonic epithelial cell growth in germ-free mice. We disclosed that histamine plays a role in oxidative stress response in R. orithinolytica.

Research Products

• [Journal Article] D-Tagatose inhibits the growth and biofilm formation of Streptococcus mutans (2018)
  • Author(s): Khaleque Hasibul, Haruyuki Nakayama-Imaohji, Masahito Hashimoto, Hisashi Yamasaki, Takaaki Ogawa, Junpei Waki, Ayano Tada, Soari Yoneda, Masaaki Tokuda, Minoru Miyake, Tomomi Kuwahara
  • Journal Title: Molecular Medicine Reprots Volume: １７ Pages: 843-851
  • DOI: 10.3892/mmr.2017.8017
  • Peer Reviewed
• [Journal Article] L-histidine augments the oxidative damage against Gram-negative bacteria by hydrogen peroxide. (2018)
  • Author(s): Tamiko Nagao，Haruyuki Nakayama-Imaohji，Miad Elahi，Ayano Tada，Emika Toyonaga，Hisashi Yamasaki，Katsuichiro Okazaki，Hirokazu Miyoshi，Koichiro Tsuchiya，Tomomi Kuwahara.
  • Journal Title: International Journal of Molecular Medicine Volume: 41 Pages: 2847-2854
  • DOI: 10.3892/ijmm.2018.3473
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of a recombinant Bacteroides fragilis sialidase expressed in Escherichia coli (2018)
  • Author(s): Yamamoto Takaaki、Ugai Hideyo、Nakayama-Imaohji Haruyuki、Tada Ayano、Elahi Miad、Houchi Hitoshi、Kuwahara Tomomi
  • Journal Title: Anaerobe Volume: 50 Pages: 69-75
  • DOI: 10.1016/j.anaerobe.2018.02.003
  • Peer Reviewed
• [Journal Article] Microbicidal effects of weakly acidified chlorous acid water against feline calicivirus and Clostridium difficile spores under protein-rich conditions (2017)
  • Author(s): Goda Hisataka、Yamaoka Hitoshi、Nakayama-Imaohji Haruyuki、Kawata Hiroyuki、Horiuchi Isanori、Fujita Yatsuka、Nagao Tamiko、Tada Ayano、Terada Atsushi、Kuwahara Tomomi
  • Journal Title: PLoS One Volume: 12 Issue: 5 Pages: 176718-176718
  • DOI: 10.1371/journal.pone.0176718
  • Peer Reviewed / Open Access
• [Journal Article] TLR2-stimulating contaminants in glycoconjugate fractons prepared from Bacteroides fragilis. (2017)
  • Author(s): Hashimoto M, Waki J, Nakayama-Imaohji H, Ozono M, Hashiguchi S, Kuwahara T.
  • Journal Title: Innate Immun Volume: 23 Issue: 5 Pages: 449-458
  • DOI: 10.1177/1753425917714313
  • Peer Reviewed
• [Journal Article] Identification of a New Virulent Clade in Enterohemorrhagic Escherichia coli O26:H11/H- Sequence Type 29. (2017)
  • Author(s): Ishijima N, Lee KI, Kuwahara T, Nakayama-Imaohji H, Yoneda S, Iguchi A, Ogura Y, Hayashi T, Ohnishi M, Iyoda S.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 43136-43136
  • DOI: 10.1038/srep43136
  • Peer Reviewed / Open Access
• [Journal Article] Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis. (2016)
  • Author(s): Bernard KA, Pacheco AL, Loomer C, Burdz T, Wiebe D, Huynh C, Kaplen B, Olson AB, Cnockaert M, Eguchi H, Kuwahara T, Nakayama-Imaohji H, Shiota H, Boudewijns M, Van Hoecke F, Vandamme P.
  • Journal Title: Int J Syst Evol Microbiol Volume: 66 Issue: 8 Pages: 2803-2812
  • DOI: 10.1099/ijsem.0.001059
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Counterselection employing mutated pheS for markerless genetic deletion in Bacteroides species (2016)
  • Author(s): Kino Y, Nakayama-Imaohji H, Fujita M, Tada A, Yoneda S, Murakami K, Hashimoto M, Hayashi T, Okazaki K, Kuwahara T.
  • Journal Title: Anaerobe Volume: 42 Pages: 81-88
  • DOI: 10.1016/j.anaerobe.2016.09.004
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Strain-specific transmission in an outbreak of ESBL-producing Enterobacteriaceae in the hemato-oncology care unit: a cohort study. (2016)
  • Author(s): Uemura M, Imataki O, Uchida S, Nakayama-Imaohji H, Ohue Y, Matsuka H, Mori H, Dobashi H, Kuwahara T, Kadowaki N.
  • Journal Title: BMC Infect Dis Volume: 17 Issue: 1 Pages: 26-26
  • DOI: 10.1186/s12879-016-2144-4
  • Peer Reviewed / Open Access
• [Journal Article] Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments. (2016)
  • Author(s): Yamaoka H, Nakayama-Imaohji H, Horiuchi I, Yamasaki H, Nagao T, Fujita Y, Maeda H, Goda H, Kuwahara T.
  • Journal Title: Lett. Appl. Microbiol. Volume: 62 Issue: 1 Pages: 47-54
  • DOI: 10.1111/lam.12506
  • Peer Reviewed
• [Journal Article] DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis. (2016)
  • Author(s): Nakayama-Imaohji H, Hirota K, Yamasaki H, Yoneda S, Nariya H, Suzuki M, Secher T, Miyake Y, Oswald E, Hayashi T, Kuwahara T
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0148887-e0148887
  • DOI: 10.1371/journal.pone.0148887
  • NAID: 120006534969
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cleansing effect of acidic L-arginine on human oral biofilm. (2016)
  • Author(s): Tada A, Nakayama-Imaohji H, Yamasaki H, Hasibul K, Yoneda S, Uchida K, Nariya H, Suzuki M, Miyake M, Kuwahara T
  • Journal Title: BMC Oral Health Volume: 16 Issue: 1 Pages: 40-40
  • DOI: 10.1186/s12903-016-0194-z
  • Peer Reviewed / Open Access
• [Presentation] Raoultella ornithinolyticaにおけるヒスタミン産生制御 (2017)
  • Author(s): 今大路治之、大岡唯祐、後藤恭宏、小椋義俊、林 哲也、桑原知巳
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター展示棟
  • Year and Date: 2017-03-19
• [Presentation] デキストラン硫酸ナトリウム処理マウスでの非O157腸管出血性大腸菌の病原性の比較 (2017)
  • Author(s): 今大路治之，伊豫田 淳，綿引正則、大西 真、桑原知巳
  • Organizer: 第70回日本細菌学会中国・四国支部総会
• [Presentation] 抗菌薬投与前後における乳幼児腸内フローラの機能メタゲノム解析 (2017)
  • Author(s): 今大路治之、田中彩、下野隆一、豊田敦、高見英人、桑原知巳
  • Organizer: 第１２回日本ゲノム微生物学会年会
• [Presentation] 機能メタゲノム解析から見た抗菌薬投与前後の乳幼児腸内フローラ (2017)
  • Author(s): 今大路治之、田中彩、下野隆一、豊田敦、高見英人、桑原知巳
  • Organizer: 第９１回日本細菌学会総会
• [Presentation] Bacteroidesのpromoter inversionによる表層変異機構 (2016)
  • Author(s): 新井貴大、今大路治之、岡崎勝一郎、桑原知巳
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis. (2016)
  • Author(s): Haruyuki Nakayama-Imaohji, Tomomi Kuwahara
  • Organizer: ASM microbe 2016
  • Place of Presentation: Boston, USA
  • Year and Date: 2016-06-16
  • Int'l Joint Research
• [Presentation] Bacteroidesにおける変異型pheS遺伝子を用いた遺伝子組換えシステムの開発 (2016)
  • Author(s): 今大路治之、藤田雅、岡崎勝一郎、桑原知巳
  • Organizer: 第８９回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府）
  • Year and Date: 2016-03-23
• [Presentation] Bacteroides fragilisにおけるシアリダーゼとエステラーゼは腹腔内膿瘍形成に関与する (2015)
  • Author(s): 今大路 治之、桑原 知巳
  • Organizer: 第85回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 奈良春日野国際フォーラム、奈良ホテル（奈良県）
  • Year and Date: 2015-10-15