| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, inhibitory effects of water extract from C. jabara peel (JWE) on lipid accumulation of 3T3-L1 adipocytes were investigated. Three active polymethoxyflavonoids, 3,5,6,7,8,3',4’-heptametoxyflavone (1), 3-hydroxy-5,6,7,8,3',4'-hexametoxyflavone (2) and 3,5,6,7,3',4'-hexametoxyflavone (3) were isolated from JWE. These compounds were significantly decreased lipid accumulation on 3T3-L1 cells. These compounds suppressed the GPDH induced-activity in a dose-dependent manner. Moreover, lipolytic activity of compounds on 3T3-L1 adipocytes was determined by measuring glycerol levels. These compounds enhanced the lipolytic effect.
The effects of compound 1 on mRNA expression of adipogenesis-related gene in 3T3-L1 adipocytes were investigated. The mRNA expression of PPARgannma and GLUT4 were markedly down-regulated by 1. On this results, compound 1 suppressed lipid accumulation through down-regulating the expression of PPARgannma and GLUT4 in 3T3-L1 adipocytes.
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