Development of molecularly-based signals from food products using LC-MS in foodomics

• Project/Area Number: 15K00879
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Ritsumeikan University
• Principal Investigator: Inoue Koichi 立命館大学, 薬学部, 准教授 (30339519)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 食品汚染 / LC-MS / 多変量解析 / 解析・評価 / 栄養学 / 薬学 / 分析科学

Outline of Final Research Achievements

In this study including the field of metabolomics for food defense, the novel evaluation of low-molecular-weight compounds using untargeted LC coupled with electrospray time-of-flight MS and multivariate statistical analysis method is proposed for the quality assessment of the classification, contamination and degradation of food products (infant formula). The LC-MS is used to monitor more detected numbers in food samples. Based on these properties of infant formula, significant differences in the classification of types and origins, the contamination of unknown compound as melamine and the degradations for one week were evaluated based on the PCA patterns. With the metabolomics strategy, the difference from the low-molecular-weight compounds could be utilized for the evaluation of the classification, contamination and degradation of infant formulas for the food safety and quality.

Research Products

• [Journal Article] Analysis of the gut microbiome and plasma short-chain fatty acid profiles in a spontaneous mouse model of metabolic syndrome (2017)
  • Author(s): Analysis of the gut microbiome and plasma short-chain fatty acid profiles in a spontaneous mouse model of metabolic syndrome.
  • Journal Title: Sci Rep. Volume: 7 Issue: 1 Pages: 15876
  • DOI: 10.1038/s41598-017-16189-5
  • NAID: 120006535024
  • URL: https://tokushima-u.repo.nii.ac.jp/records/2005544
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Principal component analysis of molecularly-based signals from infant formula contaminations using LC-MS and NMR in foodomics. (2016)
  • Author(s): Inoue, K., Tanada, C., Hosoya, T., Yoshida, S., Akiba, T., Min, J.Z., Todoroki, K., Yamano, Y., Kumazawa, S., Toyo'oka, T.
  • Journal Title: J. Sci. Food Agric. Volume: 96 Issue: 11 Pages: 3876-3881
  • DOI: 10.1002/jsfa.7584
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Metabolomics approach of infant formula for the evaluation of contamination and degradation using hydrophilic interaction liquid chromatography coupled with mass spectrometry. (2015)
  • Author(s): Inoue K, Tanada C, Sakamoto T, Tsutsui H, Akiba T, Min JZ, Todoroki K, Yamano Y, Toyo'oka T.
  • Journal Title: Food Chem Volume: 181 Pages: 318-324
  • DOI: 10.1016/j.foodchem.2015.02.117
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Principal component analysis of molecularly-based signals from infant formula contaminations using LC-MS in foodomics (2018)
  • Author(s): Inoue, K., Nagatomo, R., Tanakda, C., Harada, S.
  • Organizer: PITTCON 2018 Conference & Expo.
  • Int'l Joint Research
• [Presentation] フードミクスとは？「食の安全から機能性探索までの応用」 (2015)
  • Author(s): 井之上浩一
  • Organizer: ifia JAPAN2015
  • Place of Presentation: 東京ビッグサイト（東京・江東区）
  • Year and Date: 2015-05-20
  • Invited
• [Book] 月刊フードケミカル (2016)
  • Author(s): 井之上浩一
  • Total Pages: 118
  • Publisher: 食品化学新聞社
• [Book] Advanced Mass Spectrometry for Food Safety and Quality 1st Edition (2015)
  • Author(s): Inoue K., Toyo'oka T.
  • Total Pages: 713
  • Publisher: ELSEVIER