(-)-epigallocatechin-3-gallate protects against neuronal cell death and improves cerebralfunction after traumatic brain injury in aged rats

• Project/Area Number: 15K00898
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Kindai University
• Principal Investigator: SATOU Takao 近畿大学, 医学部附属病院, 教授 (70162443)
• Co-Investigator(Kenkyū-buntansha): 井上 敬夫 近畿大学, 医学部, 助教 (00441006) ; 伊藤 龍生 近畿大学, 農学部, 教授 (40330245)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 緑茶 / 高齢者 / 脳外傷 / 神経保護 / 酸化ストレス / EGCG / 老齢ラット / 神経再生 / 高次脳機能障害 / 認知症

Outline of Final Research Achievements

A major component of green tea, a widely consumed beverage, is EGCG, which has strong antioxidant properties. In this study, we investigated the effects of EGCG on cerebral function and morphology following TBI. 24-month-old rats male Wistar rats that had access to normal drinking water, or water containing 0.1% (w/v) EGCG ad libitum, received TBI with a pneumatic controlled injury device for 4weeks. Immunohistochemistry and lipid peroxidation studies revealed that at 1, 3 and 7 days post-TBI, the number of 8-OHdG-, 4-HNE- and ssDNA-positive cells, and the levels of MDA around the damaged area after TBI, significantly decreased in the EGCG treatment group compared with the water group. In addition, there was a significant increase in the number of surviving neuronal cells and an improvement in cerebral dysfunction after TBI in the EGCG treatment group compared with the water group. In summary, consumption of green tea may be an effective therapy for TBIaged patients.

Research Products

• [Journal Article] Contributions of MET activation to BCR-ABL1 tyrosine kinase inhibitor resistance in chronic myeloid leukemia cells. (2017)
  • Author(s): Tsubaki M, Takeda T, Kino T, Sakai K, Itoh T, Imano M, Nakayama T, Nishio K, Satou T, Nishida S.
  • Journal Title: Oncotarget. Volume: 8 Issue: 24 Pages: 38717-38730
  • DOI: 10.18632/oncotarget.16314
  • Peer Reviewed / Open Access
• [Journal Article] The sensitivity of head and neck carcinoma cells to statins is related to the expression of their Ras expression status, and statin-induced apoptosis is mediated via suppression of the Ras/ERK and Ras/mTOR pathways. (2017)
  • Author(s): Tsubaki M, Fujiwara D, Takeda T, Kino T, Tomonari Y, Itoh T, Imano M, Satou T, Sakaguchi K, Nishida S.
  • Journal Title: Clin Exp Pharmacol Physiol. Volume: 44 Issue: 2 Pages: 222-234
  • DOI: 10.1111/1440-1681.12690
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Statins inhibited the MIP-1α expression via inhibition of Ras/ERK and Ras/Akt pathways in myeloma cells. (2016)
  • Author(s): Tsubaki M, Mashimo K, Takeda T, Kino T, Fujita A, Itoh T, Imano M, Sakaguchi K, Satou T, Nishida S.
  • Journal Title: Biomed Pharmacother. Volume: 78 Pages: 23-29
  • DOI: 10.1016/j.biopha.2015.12.017
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mangiferin enhances the sensitivity of human multiple myeloma cells to anticancer drugs through suppression of the nuclear factor κB pathway (2016)
  • Author(s): Takeda T, Tsubaki M, Kino T, Kawamura A, Isoyama S, Itoh T, Imano M, Tanabe G, Muraoka O, Satou T, Nishida S
  • Journal Title: Int J Oncol Volume: 48 Issue: 6 Pages: 303-309
  • DOI: 10.3892/ijo.2016.3470
  • Peer Reviewed / Open Access
• [Journal Article] A phase II trial of perioperative chemotherapy involving a single intraperitoneal administration of paclitaxel followed by sequential S-1 plus intravenous paclitaxel for serosa-positive gastric cancer (2015)
  • Author(s): Peng YF, Imano M, Itoh T, Satoh T, Chiba Y, Imamoto H, Tsubaki M, Nishida S, Yasuda T, Furukawa H
  • Journal Title: J Surg Oncol Volume: 111 Issue: 8 Pages: 1041-1046
  • DOI: 10.1002/jso.23928
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mangiferin suppresses CIA by suppressing the expression of TNF-α, IL-6, IL-1β, and RANKL through inhibiting the activation of NF-κB and ERK1/2 (2015)
  • Author(s): Tsubaki M, Takeda T, Kino T, Itoh T, Imano M, Tanabe G, Muraoka O, Satou T, Nishida S
  • Journal Title: Am J Transl Res Volume: 7 Pages: 1371-1381
  • Peer Reviewed
• [Journal Article] Statins improve survival by inhibiting spontaneous metastasis and tumor growth in a mouse melanoma model (2015)
  • Author(s): Tsubaki M, Takeda T, Kino T, Obata N, Itoh T, Imano M, Mashimo K, Fujiwara D, Sakaguchi K, Satou T, Nishida S
  • Journal Title: Am J Cancer Res Volume: 5 Pages: 3186-3197
  • Peer Reviewed
• [Presentation] 高血圧曝露によるラット胎仔神経幹細胞の発達・分化への影響 (2017)
  • Author(s): 藪口友暉、蒲尚子、水口信行、伊藤龍生、佐藤隆夫
  • Organizer: 第71回日本栄養・食糧学会
• [Presentation] 運動による脳外傷後の脳機能改善効果の検討 (2017)
  • Author(s): 吉田原規、伊藤龍生、橋本重夫、土手健作、佐藤隆夫
  • Organizer: 第106回日本病理学会総会大会
• [Presentation] 母体高血糖がラット胎仔神経幹細胞分化に及ぼうす影響 (2016)
  • Author(s): 藤原隆史、吉田大亮、福田麻美、藪口友暉、金田雅大、森万優加、小倉あづき、足立夏実、水口信行、佐藤隆夫、伊藤龍生
  • Organizer: 第55回日本栄養・食糧学会・近畿支部大会
  • Place of Presentation: 帝塚山学院大学(大阪府、堺市）
  • Year and Date: 2016-10-22
• [Presentation] 運動による実験的脳外傷後に出現する神経幹細胞への影響 (2015)
  • Author(s): 伊藤龍生、橋本重夫、佐藤隆夫
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-04-29
• [Presentation] 外傷的脳損傷の損傷局所に及ぼす運動の影響について (2015)
  • Author(s): 佐藤隆夫、伊藤龍生、土手健作、橋本重夫
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-04-29