| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Annual Research Achievements |
After several experiments the intended model compound i.e. the structural motif that would be linked to the polymer, was isolated. The model compound consists of a phenyl ring with two CH(SCOCH3)2 groups in ortho position relative to each other and its structure was confirmed by NMR and HRMS measurements. The compound was able to release H2S in the presence of glutathione as detected in the gas phase as lead sulfide. More important it could be shown that in the presence of glutathione, the major thiol present inside the cell, also showed a time-dependent increase in fluorescence showing that the proposed concept is working. To show that this fluorescence is due to the proposed adduct formed by reaction of glutathione with the formed dialdehyde after H2S release, the measured emission scan was the same as that measured for the product obtained after reacting glutathione with the dialdehyde directly. Furthermore the isomer having the CH(SCOCH3)2 groups in para position did release H2S but no increase in fluorescence was detected after reaction with glutathione. This results support the proposed reaction mechanism. Cell experiment in mouse macrophages showed both compounds not to be toxic up to 100 micromolar. Confocal laser scanning electron microcopy was able to detect weak fluorescence for both the ortho and para isomer because of auto-fluorescence of the cells.
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