Development of Quality Control Method in Regenerative Medicine Products Using a Bioradiography
| Project/Area Number |
15K01297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kitasato University |
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Principal Investigator |
Sasaki Toru 北里大学, 医療衛生学部, 准教授 (30158927)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 還元ストレス / 活性酸素 / レドックス / 酸化ストレス / 再生医療 / 分子イメージング / 品質管理 / 陽電子放出核種 |
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| Outline of Final Research Achievements |
We investigated reactive oxygen species (ROS) generation in cerebral cortex slices during the restoration of hypoxia caused by a decreased oxygen supply and a transiently enhanced energy metabolism, with tissue pO2 changes and redox balance using real-time bioradiography. ROS generation was transiently enhanced during reoxygenation in both hypoxic cases. The redox balance shift towards reduction “hyper-reduction” in tissue were suggested to participate in increased ROS generation under the restoration of transient hypoxia induced by a decreased oxygen supply and increased oxygen demand. To investigate whether it is possible to estimate the safety, soundness and effectiveness of transplantable tissues and organs by FDG-bioradiography method in a non-invasive manner, we examined the FDG uptake in reconstructed human cultured epidermal model. This result suggested the possibility of quality control in regenerative medical products using FDG - biodiography.
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Report
(4 results)
Research Products
(12 results)
