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Skin tissue bud formation by in vitro self-organization approach

Research Project

Project/Area Number 15K01303
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionOkayama University of Science

Principal Investigator

Kataoka Ken  岡山理科大学, 理学部, 教授 (10293317)

Co-Investigator(Kenkyū-buntansha) 辻極 秀次  岡山理科大学, 理学部, 教授 (70335628)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords附属器原基 / 培養皮膚 / 培養皮膚組織 / 附属器 / 組織原基 / 組織形成
Outline of Final Research Achievements

Human mesenchymal stem cells, human endothelial cells, mouse skin dermal cells and epidermal cells were cultured with/without mouse macrophages on Matrigel-coated culture plates in vitro. Inoculated cells were aggregated and formed spheroids with layers. When the spheroids were transplanted on the back of immune-deficient mice, very few fair follicles were observed at the transplantation site.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes2018

    • Author(s)
      Kataoka Ken、Maehara Natsumi、Ayabe Yuki、Murata Hitoshi、Huh Nam-Ho、Sakaguchi Masakiyo
    • Journal Title

      Mol Med Rep.

      Volume: 17 Pages: 6661-6666

    • DOI

      10.3892/mmr.2018.8676

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spontaneous hair follicle germ (HFG) formation in vitro, enabling the large-scale production of HFGs for regenerative medicine2018

    • Author(s)
      Kageyama T, Yoshimura C, Myasnikova D, Kataoka K, Nittami T, Maruo S, Fukuda J
    • Journal Title

      Biomaterials

      Volume: 154 Pages: 291-300

    • DOI

      10.1016/j.biomaterials.2017.10.056

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of tumor suppressor REIC/Dkk-3 by a newly improved adenovirus vector with insertion of an hTERT promoter at the 3’-side of the transgene.2017

    • Author(s)
      Putranto EW, Kinoshita R, Watanabe M, Sadahira T, Murata H, Yamamoto KI, Futami J, Kataoka K, Inoue Y, Ruma IM, Sumardika IW, Youyi C, Kubo M, Sakaguchi Y, Saito K, Nasu Y, Kumon H, Huh NH, and Sakaguchi M.
    • Journal Title

      Oncol. Lett.

      Volume: 14 Issue: 1 Pages: 1041-1048

    • DOI

      10.3892/ol.2017.6201

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 本当に知ってる?正しい細胞培養の手法122016

    • Author(s)
      片岡健
    • Journal Title

      PHARM TECH JAPAN

      Volume: 32 Pages: 57-58

    • Related Report
      2016 Research-status Report
  • [Journal Article] Expression pattern of Dkk-3, a secreted Wnt pathway inhibitor, in mouse intestinal tissue and three-dimensional cultured Caco-2 spheroids2015

    • Author(s)
      Kataoka K, Rikitake Y, Ayabe Y
    • Journal Title

      J Stem Cells Regen Med

      Volume: 11 Pages: 48-50

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] NRF2 regulates PINK1 expression under oxidative stress conditions2015

    • Author(s)
      Murata H, Takamatsu H, Liu S, Kataoka K, Huh NH, Sakaguchi M
    • Journal Title

      PLoS One

      Volume: 10

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 細胞培養における基礎知識と注意点2015

    • Author(s)
      片岡 健
    • Journal Title

      日本臨床エンブリオロジスト学会雑誌

      Volume: 17 Pages: 1-4

    • Related Report
      2015 Research-status Report
  • [Presentation] ニワトリ胎仔より調製した表皮シート・真皮細胞の3次元培養によるin vitro羽毛芽の形成2017

    • Author(s)
      清住哲也、多田真由美、辻極秀次、片岡健
    • Organizer
      日本組織培養学会第90回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脱毛治療のためのin vitro毛髪再生2017

    • Author(s)
      清水 亮啓、景山 達斗、片岡 健、平川 聡史、福田 淳二
    • Organizer
      日本組織培養学会第90回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 赤血球形態・Fe染色・鉄代謝を用いたACD、low grade MDSの鑑別2016

    • Author(s)
      西山翔、中條早智子,大野明里、田中正信、片岡健
    • Organizer
      日本臨床衛生検査技師会中四国支部医学検査学会(第49回)
    • Place of Presentation
      高知分化プラザかるぽーと
    • Year and Date
      2016-11-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Student-led approaches for sustained HIV/AIDS awareness in Okayama University of Science2016

    • Author(s)
      Ken Kataoka, Yumi Okamoto, Hisaharu Tanaka, Tatsunori Tamaki
    • Organizer
      IFBLS 2016
    • Place of Presentation
      Kobe International Conference Center
    • Year and Date
      2016-08-31
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 皮膚組織原基の培養内作成とその移植2016

    • Author(s)
      力武祐治、鶴田太輝、綾部雄基、辻極秀次、片岡健
    • Organizer
      日本組織培養学会第89回大会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Year and Date
      2016-05-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Potential role of tumor suppressor gene REIC/Dkk-3 as a regulator of skin tissue inflammation2015

    • Author(s)
      Ayabe Y, Tsuruda T, Sakaguchi M, Kataoka K
    • Organizer
      ASCB Annual Meeting
    • Place of Presentation
      San Diego
    • Year and Date
      2015-12-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 血液疾患館別における特殊染色の有用性2015

    • Author(s)
      髙橋 都、東原美沙、藤原鉄朗、田中正信、片岡 健
    • Organizer
      第48回日本臨床衛生検査技師会中四国支部医学検査学会
    • Place of Presentation
      米子
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] TNF-αによるREIC/Dkk-3の発現制御とケラチノサイトの挙動への影響2015

    • Author(s)
      綾部雄基、鶴田太輝 、阪口政清 、 片岡 健
    • Organizer
      日本組織培養学会第88回大会
    • Place of Presentation
      広島
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
  • [Presentation] In vitro自己組織化による皮膚組織原基の形成2015

    • Author(s)
      鶴田太輝、 綾部雄基 、辻極秀次 、 片岡 健
    • Organizer
      日本組織培養学会第88回大会
    • Place of Presentation
      広島
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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