| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The linker of nucleoskeleton and cytoskeleton (LINC) complex has been suggested to be a mechanical link tethering the nucleus to cytoskeletons in a cell. In this study, we address the role of nesprin-1, which is a component of LINC complex and directly binds to actin cytoskeletons, -medicated nucleus-actin filament bindings in morphological changes of fibroblasts exposed to cyclic stretching. Fibroblasts were transfected with siRNA against nesprin-1 and used for cyclic stretching experiments. After exposure to cyclic stretching, siRNA-treated fibroblasts showed less elongated shapes compared to non-treated wild type cells. Treatment of Trichostatin A, which decreases nuclear stiffness, induced more rounded shapes than those of non-treated cells under both static and cyclic stretching conditions. These results suggest that nucleus-actin filaments bindings play an important mechanical role in formation of elongated shapes of fibroblasts under cyclic stretching condition.
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