| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
In this study, we developed a polymeric Gd-conjugated contrast agent for high-resolution imaging of cerebrovasculature. The branched polyethylene glycols having approximately 15 kDa of molecular weight were conjugated with Gd-chelate and fluorescein. We showed that microvasculature network could be visualized with the resolution of 100 μm by using the contrast agents. Experimental results indicated that relaxivity increasing was induced by the branch structure, and the agents were excreted in urine after 24 hours for the administration. Inflammatory markers in the blood were not induced by the injection. Moreover, we succeeded in proving the possibility that cerebral ischemia region and collateral flow were clearly visualized with the polymeric contrast agents in middle cerebral artery occlusion model rats.
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