| Project/Area Number |
15K01343
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | Yokohama College of Pharmacy |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岩瀬 由未子 横浜薬科大学, 薬学部, 講師 (00521882)
梅村 晋一郎 東北大学, 医工学研究科, 教授 (20402787)
弓田 長彦 横浜薬科大学, 薬学部, 教授 (40191481)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 超音波 / がん指向性カーボンナノチューブ / アポトーシス / 音響化学療法 / ナノ粒子 / ナノカーボンチューブ / アネキシンV染色 / がん指向性ナノチューブ |
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| Outline of Final Research Achievements |
The present study aims to investigate sonodynamically-induced apoptosis using the Polyhydroxy Carbon Nanotube (PHCN). HL-60 cells were exposed to ultrasound in the absence and presence of PHCN. Apoptosis was analyzed by cell morphology and caspase-3 activity. The number of apoptotic cells showing membrane blebbing and cell shrinkage after combined treatment (ultrasound and PHCN) was significantly higher than following other treatments, including ultrasound alone and PHCN alone. Furthermore, Enhancement of caspase-3 activation was observed in cells treated with ultrasound and PHCN. Sonodynamically induced apoptosis and caspase-3 activation were significantly suppressed by histidine. These results indicate that the combination of ultrasound and PHCN induced apoptosis by reactive active oxygen in HL-60 cells.
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