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Basic study for prevention of disuse muscular atrophy with multivalent cations

Research Project

Project/Area Number 15K01401
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Rehabilitation science/Welfare engineering
Research InstitutionKansai University of Welfare Sciences

Principal Investigator

Mori Yoshiaki  関西福祉科学大学, 保健医療学部, 教授 (70268192)

Co-Investigator(Kenkyū-buntansha) 山路 純子 (田代純子)  関西福祉科学大学, 健康福祉学部, 教授 (40340559)
廣島 玲子  関西福祉科学大学, 保健医療学部, 准教授 (40404777)
宮崎 彩子  兵庫医科大学, 医学部, 准教授 (20298772)
Project Period (FY) 2015-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords骨格筋細胞 / カルシニューリン / インターロイキン-6 / ミオシン重鎖アイソフォームI / 多価陽イオン / 廃用性筋萎縮 / ミオシン重鎖タイプI / IL-6 / 骨格筋 / イオンチャネル / La3+ / Gd3+ / Ni2+
Outline of Final Research Achievements

Using the RT-PCR method, we tried to examine the effects of multivalent cations on expression levels of interleukin-6 (IL-6) and myosin heavy chain I (MyHC I) mRNA in C2C12 cells. These mRNA expression levels were significantly increased by La3+, Gd3+, or Ni2+. The increased mRNA levels of MyHC I and IL-6 induced by the administration of these multivalent cations were attenuated by the co-administration of cyclosporine A. Furthermore, the effects of La3+ on MyHC I mRNA levels were attenuated by the co-administration of anti-IL-6 receptor antibody. Addition to the RT-PCR study, we measured whole-cell currents in C2C12 cells using patch-clamp technique. The inward currents were not significantly affected by the application of La3+ to the bath solution. These results indicated that a small quantity of multivalent cations into the cytosol upregulates IL-6 mRNA level in calcineurin dependent manner, and increment of IL-6 production from myocites upregulates MyHC I mRNA expression level.

Academic Significance and Societal Importance of the Research Achievements

本研究では,La3+などの多価陽イオンの投与によるカルシニューリン活性化がIL-6のmRNA発現量を増加させ,骨格筋細胞自身がIL-6を分泌することでMyHC IのmRNA発現量を増加させることを明らかにした。今後の検討課題は,細胞外への多価陽イオンの投与が実際にMyHC Iタンパク質を増加させるかを,培養細胞のみならず実験動物を用いて確認することである。これらの検討を行うことで,正常な動物の骨格筋維持における微量元素の細胞生物学的な役割を確認できるとともに,廃用性筋萎縮の予防法やリハビリテーションの有効性を高める補助治療法の開発が可能になるものと考える。

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (32 results)

All 2020 2019 2018 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (26 results) (of which Int'l Joint Research: 11 results)

  • [Journal Article] CGRP upregulates myosin heavy chain type I messenger RNA through calcineurin-IL-6-independent manner in C2C12 cells.2018

    • Author(s)
      Yoshiaki Mori, Junko Yamaji
    • Journal Title

      Journal of Neuromuscular Diseases

      Volume: 5 Issue: s1 Pages: S1-S408

    • DOI

      10.3233/jnd-189001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 侵害受容における電気生理学の基礎知識2018

    • Author(s)
      森 禎章, 山路 純子
    • Journal Title

      保健医療学雑誌

      Volume: 9(1) Pages: 45-61

    • NAID

      130006602970

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Upregulation of myosin heavy chain type I and interleukin-6 mRNA levels by exogenous application of calcineurin activators in C2C12 skeletal myocytes.2016

    • Author(s)
      Yoshiaki Mori, Junko Yamaji, Reiko Hiroshima, Ayako Miyazaki
    • Journal Title

      Journal of Neuromuscular Diseases

      Volume: 3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of interleukin-6 on upregulation of myosin heavy chain type IIb messenger RNA in mouse myocytes.2016

    • Author(s)
      Junko Yamaji, Yoshiaki Mori, Reiko Hiroshima, Ayako Miyazaki
    • Journal Title

      Journal of Neuromuscular Diseases

      Volume: 3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] The value of weight-bearing rate on the paretic lower limb for an independent walking without a cane in patients with stroke.2015

    • Author(s)
      Akezaki Y, Mori Y, Nomura T, Nagino K, Aramaki R, Nakata E.
    • Journal Title

      JAHS

      Volume: 6 Pages: 36-42

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Blood supply-susceptible formation of melanin pigment in hair bulb melanocytes of mice.2015

    • Author(s)
      Maeda S, Ueda K, Yamana H, Tashiro-Yamaji J, Ibata M, Mikura A, Okada M, Yasuda E, Shibayama Y, Yoshino M, Kubota T, Yoshida R.
    • Journal Title

      Plast Reconstr Surg Glob Open

      Volume: e328 Issue: 3 Pages: 1-9

    • DOI

      10.1097/gox.0000000000000284

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Activation CGRP-cAMP-dependent signal transduction pathways upregulate MyHC I mRNA in C2C12 cells.2020

    • Author(s)
      森 禎章、山路純子、廣島玲子
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Role of cyclic AMP pathway on expression of MyHC II and IL-6 mRNAs in mouse myocytes2020

    • Author(s)
      山路純子、廣島玲子、森 禎章
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] C2C12 細胞におけるカルシトニン関連遺伝子ペプチド(CGRP)のミオシン重鎖タイプ I に対する作用2019

    • Author(s)
      森 禎章、山路純子、廣島玲子
    • Organizer
      日本筋学会 第5回学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マウス骨格筋細胞における IL-6 及びミオシン重鎖クラス II のmRNA 発現へのカルシトニン遺伝子関連ペプチド(CGRP)の作用2019

    • Author(s)
      山路純子、森 禎章、廣島玲子
    • Organizer
      日本筋学会 第5回学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Application of CGRP upregulates MyHC I mRNA through cAMP-dependent manner in C2C12 cells.2019

    • Author(s)
      Yoshiaki Mori, Junko Yamaji
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies Congress
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Essential role of calcineurin but not cAMP in mRNA expression of MyHC II and IL-6 in murine myocytes.2019

    • Author(s)
      Junko Yamaji, Yoshiaki Mori
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies Congress
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] CGRP upregulates myosin heavy chain type I messenger RNA through calcineurin-IL-6-independent manner in C2C12 cells.2018

    • Author(s)
      Yoshiaki Mori, Junko Yamaji
    • Organizer
      15th International Congress on Neuromuscular Diseases
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Modulating effects of unsaturated fatty acids on gene expression of myosin heavy chain class I and IIB in C2C12 myocytes.2018

    • Author(s)
      Junko Yamaji, Yoshiaki Mori
    • Organizer
      15th International Congress on Neuromuscular Diseases
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Application of CGRP upregulates MyHC I mRNA through IL-6 independent manner in C2C12 cells.2018

    • Author(s)
      森 禎章, 山路 純子, 廣島 玲子
    • Organizer
      第95回日本生理学会大会,高松
    • Related Report
      2017 Research-status Report
  • [Presentation] The effect of calcitonin gene-related peptide (CGRP) on mRNA levels of myosin heavy chain class II (MyHC II) and interleukin (IL) -6 in mouse myocytes.2018

    • Author(s)
      山路 純子, 廣島 玲子, 森 禎章
    • Organizer
      第95回日本生理学会大会,高松
    • Related Report
      2017 Research-status Report
  • [Presentation] Upregulatiion mechanisms of myosin heavy chain type I mRNA induced by exogenous application of calcineurin activators in C2C12 skeletal myocytes.2017

    • Author(s)
      森 禎章,山路 純子,廣島 玲子
    • Organizer
      第94回 日本生理学会大会
    • Place of Presentation
      浜松(静岡)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] The effect of interleukin (IL) -6 and long-chain unsaturated fatty acids on mRNA levels of myosin heavy chain class IIb (MyHC IIb) in murine myocytes.2017

    • Author(s)
      山路 純子,森 禎章,廣島 玲子
    • Organizer
      第94回 日本生理学会大会
    • Place of Presentation
      浜松(静岡)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Exogenous application of La3+ upregulates myosin heavy chain type I mRNA through activation of calcineurin in C2C12 cells.2017

    • Author(s)
      Yoshiaki Mori, Junko Yamaji
    • Organizer
      The 46th European Muscle Conference, Potsdam
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effect of calcineurin activation by organic acids on expression of interleu- kin-6 and myosin heavy chain class IIb mRNA levels in mouse myocytes.2017

    • Author(s)
      Junko Yamaji, Yoshiaki Mori
    • Organizer
      The 46th European Muscle Conference, Potsdam
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] マウス骨格筋におけるミオシン重鎖クラス IIb mRNA 発現への不飽和脂肪酸の作用.2017

    • Author(s)
      岡本 奈緒, 山路 純子, 坂上 裕介, 岩元 歩, 森 禎章
    • Organizer
      第8回 保健医療学学会,寝屋川
    • Related Report
      2017 Research-status Report
  • [Presentation] 無呼吸負荷により生じる蝸牛内直流電位低下におけるCキナーゼの役割2017

    • Author(s)
      森 禎章,乾 崇樹,渡辺正仁,河田 了
    • Organizer
      第7回 総合福祉科学学会 学術集会
    • Place of Presentation
      柏原(大阪)
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス骨格筋細胞におけるIL-6およびミオシン重鎖タイプI・IIのmRNA発現へのクロロゲン酸の作用2017

    • Author(s)
      山路 純子,廣島 玲子,森 禎章
    • Organizer
      第7回 総合福祉科学学会 学術集会
    • Place of Presentation
      柏原(大阪)
    • Related Report
      2016 Research-status Report
  • [Presentation] UPREGULATION OF MYOSIN HEAVY CHAIN TYPE I AND INTERLEUKIN-6 MESSENGER RNA LEVELS BY EXOGENOUS APPLICATION OF CALCINEURIN ACTIVATORS IN C2C12 SKELETAL MYOCYTES2016

    • Author(s)
      Yoshiaki Mori, Junko Yamaji, Reiko Hiroshima, Ayako Miyazaki
    • Organizer
      14th International Congress of Neuromuscular Disorders(ICNMD2016)
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2016-07-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ROLE OF INTERLEUKIN-6 ON UPREGULATION OF MYOSIN HEAVY CHAIN TYPE IIB MESSENGER RNA IN MOUSE MYOCYTES2016

    • Author(s)
      Junko Yamaji, Yoshiaki Mori, Reiko Hiroshima, Ayako Miyazaki
    • Organizer
      14th International Congress of Neuromuscular Disorders(ICNMD2016)
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2016-07-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of calcineurin activators on expression levels of myosin heavy chain type I mRNA in C2C12 skeletal myocytes.2016

    • Author(s)
      森 禎章,山路純子
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Role of unsaturated fatty acids on expression of myosin heavy chain type II b mRNA in mouse myocytes.2016

    • Author(s)
      山路純子, 森 禎章, 清水宏泰
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] 廃用性筋萎縮からの回復過程において抗炎症剤がミオシン重鎖mRNA発現量に与える影響2016

    • Author(s)
      廣島玲子,山路純子,森 禎章
    • Organizer
      第6回 総合福祉科学学会
    • Place of Presentation
      関西福祉科学大学(柏原)
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
  • [Presentation] クロロゲン酸は骨格筋細胞ミオシン重鎖タイプIIb mRNA発現量を増加させる.2015

    • Author(s)
      芳仲千尋,前田優香里,河野亜紀,中西真友香,山路純子,森 禎章
    • Organizer
      保健医療学学会 第6回学術集会
    • Place of Presentation
      I-siteなんば(大阪)
    • Year and Date
      2015-11-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Enhancement of myosin heavy chain class I (MHC I) mRNA expression in C2C12 myocyte by multivalent cations.2015

    • Author(s)
      Mori Y, Yamaji J, Hiroshima R, Watanabe M, Miyazaki A
    • Organizer
      20th International Congress of World Muscle Society(WMS2015)
    • Place of Presentation
      Brighton(英国)
    • Year and Date
      2015-09-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Contribution of IL-6-dependent signaling mechanism to upregulation of MyHC IIb mRNA but not of MyHC IIa mRNA in mouse myocytes.2015

    • Author(s)
      Yamaji J, Mori Y, Hiroshima R, Watanabe M, Miyazaki A
    • Organizer
      20th International Congress of World Muscle Society(WMS2015)
    • Place of Presentation
      Brighton(英国)
    • Year and Date
      2015-09-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] mRNA and protein analysis of MHC and HSP70 in rat soleus muscle recovering from disuse atrophy.2015

    • Author(s)
      Hiroshima R, Yamaji J, Mori Y
    • Organizer
      WCPT 2015(World Confederation for Physical Therapy)
    • Place of Presentation
      Singapore(シンガポール)
    • Year and Date
      2015-05-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2021-02-19  

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